| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Our purpose of this study is to identify endogenouse ligands for orphan GPCRs by newly developed screening systems. We utilized two intracellular signaling pathways, G-proteins and β-arrestin for detection of GPCR activation. To detect β-arrestin signaling, we monitored the ligand activated GPCR-mediated β-arrestin recruitment by using enzymatic complementation of E. coli β-galactosidase, alpha and omega fragments. In terms of detection of G-protein signaling, we generated a new technology that simply monitoring activities of any G-protein alpha subunits leading to minimization of experimental steps. We have optimized these assay systems suitable for high through put screening and being conducting the screening.
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