Analysis of cerebral blood flow regulation using in vivo fluorescence imaging
| Project/Area Number |
26460335
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Sekiya Hiroshi 東京大学, 大学院医学系研究科(医学部), 助教 (40511374)
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| Co-Investigator(Renkei-kenkyūsha) |
Tanaka Kenji 慶應義塾大学, 医学部, 准教授 (30329700)
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| Research Collaborator |
Kitajima Nami 東京大学, 大学院医学系研究科(医学部), 学部学生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 血管平滑筋 / 脳血流 / カルシウムシグナル / 蛍光イメージング / アストロサイト |
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| Outline of Final Research Achievements |
The brain requires a lot of energy even in a resting state. During information processing, the brain requires extra blood flow. This increase of blood flow during neuronal activities is a fundamental phenomenon. The mechanisms of cerebral blood flow regulation, however, remain to be elusive. To clarify the regulation mechanism, we performed in vivo calcium imaging of neurons, astrocytes and vascular smooth muscle cells in the cerebral cortex of mouse and analyzed spatiotemporal dynamics of activities of these cells. In addition, to investigate the signal pathways among these cells, we performed pharmacological experiments combined with fluorescence imaging. The results of these experiments show that activities of neurons and astrocytes lead to relaxation of arterial smooth muscle cells, which causes the increase of blood flow, and that noradrenaline, as an extracellular signaling molecule, plays a key role in the regulation of blood flow dynamics.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity.2017
Author(s)
Akiyo Natsubori, Iku Tsustui-Kimura, Hiroshi Nishida, Youcef Bouchekioua, Hiroshi Sekiya, Motokazu Uchigashima, Masahiko Watanabe, Alban de Kerchove d'Exaerde, Masaru Mimura, Norio Takata, and Kenji Tanaka
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Journal Title
Journal of Neuroscience
Volume: 37
Issue: 10
Pages: 2723-2733
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Whisker experience-dependent mGluR signaling maintains synaptic strength in the mouse adolescent cortex.2016
Author(s)
Kubota, J., Mikami, Y., Kanemaru, K., Sekiya, H., Okubo, Y. and Iino, M.
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Journal Title
Eur. J. Neurosci.
Volume: 44
Issue: 3
Pages: 2004-2014
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] In vivo visualization of subtle, transient and local activity of astrocytes using an ultrasensitive Ca2+ indicator.2014
Author(s)
Kanemaru K, Sekiya H, Xu M, Satoh K, Kitajima N, Yoshida K, Okubo Y, Sasaki T, Moritoh S, Hasuwa H, Mimura M, Horikawa K, Matsui K, Nagai T, Iino M, Tanaka KF
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Journal Title
Cell Rep
Volume: 8(1),311
Issue: 1
Pages: 311-318
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] In vivo imaging analysis of astrocytic neuroprotective effects in cerebral infarction2017
Author(s)
Hiroshi Sekiya, Kenji Takikawa, Kaname Sato, Miki Takagi, Nami Kitajima, Toshiko Yamazawa, Hirokazu Sakamoto, Shigeyuki Namiki, Kazunori Kanemaru, Kenji F. Tanaka, Kenzo Hirose, Masamitsu Iino
Organizer
第90回日本薬理学会
Place of Presentation
長崎ブリックホール(長崎県長崎市)
Related Report
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