Identify the novel target molecules of valproic acid using model organism
| Project/Area Number |
26460339
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Kobe University |
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Principal Investigator |
Yan Ma 神戸大学, 医学部附属病院, 医員 (70457050)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | バルプロ酸 / 分子標的 / 分裂酵母 / TORC1経路 / 酵母モデル生物 / 遺伝子発現 / 薬物感受性 |
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| Outline of Final Research Achievements |
Valproic acid (VPA) is widely used as an anticonvulsant drug. More recently, it has been reported that VPA improved the efficiency upon iPS reprogramming and the administration of VPA dramatically enhanced the restoration of hind limb function. The exact target molecules of the various effects of VPA remain unclear. We have developed a genetic approach using S pombe as a model organism. This study led to the following findings. We identified a novel VPA sensitive mutant of vas4-1/vrg4-v4, a mutant allele of the vrg4+ gene encoding S pombe homolog of Golgi GDP-mannose transmembrane transporter, and its mammalian homologue encodes disease responsible gene of cochlear like pelvic dysplasia. We found that Vrg4 localizes to Golgi, and isolated several genes as dosage-dependent suppressors of the VPA-sensitive phenotype of vrg4-v4 mutant. Furthermore we found the expression level of many genes was markedly increased or decreased upon VPA treatment using cap analysis of gene expression.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Tor Signaling Regulates Transcription of Amino Acid Permeases through a GATA Transcription Factor Gaf1 in Fission Yeast. Ma Y, Ma N, Liu Q, Qi Y, Manabe R, Furuyashiki T.2015
Author(s)
Yan Ma, Ning Ma, Qingbin Liu, Yao Qi, Ri-ichiroh Manabe, Tomoyuki Furuyashiki
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Journal Title
PLoS One
Volume: 10
Issue: 12
Pages: e0144677-e0144677
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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