| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We here examined effects of iPS factors, Sox2 and Klf4, on expression of TRP channels in HEK cells where iPS factors were hetelorogously expressed. Sox2 induced the expression of TRPC6, TRPM1, TRPM2, and TRPV2, whereas it reduced the expression of TRPA1. On the other hand, Klf4 induced the marked expression of TRPC5, TRPC6, TRPM2, TRPM4, TRPM6, and TRPV2, whereas it reduced the expression of TRPC1. These results suggest that iPS factors, Sox2 and Klf4, regulate gene-expression of ion channels. In this study, we also demonstrate that differentiation of non-neuronal cell line neuroblastorma to neuron induces functional expression of TRPM3.
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