Elucidation of the activation mechanism of TRPA1 channel by hydrogen sulfide-derived polysulfide

• Project/Area Number: 26460352
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General pharmacology
• Research Institution: National Center of Neurology and Psychiatry
• Principal Investigator: Kimura Yuka 国立研究開発法人国立精神・神経医療研究センター, 神経研究所, 科研費研究員 (60425692)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Hideo 国立精神, 神経医療研究センター, 研究員 (30321889)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ポリサルファイド / 硫化水素 / TRPA1 / 3-メルカプトピルビン酸イオウ転移酵素 / シグナル分子 / メルカプトピルビン酸 / 脳 / レドックス調節 / 3-メルカプトピルビン酸硫黄転移酵素 / 酵素的産生 / ロダネース / 3メルカプトピルビン酸硫黄転移酵素 / Rhodanese / 細胞内カルシウム / アストロサイト / シグナリング

Outline of Final Research Achievements

We demonstrated that hydrogen sulfide(H2S) and polysulfide (H2Sn、n≧2)are endogenous signaling molecules. In the present study, we have elucidated the mechanism of activation of transient receptor potential ankyrin1 (TRPA1) channels; 1) Sulfhydration of cysteine residues in TRPA1 by H2Sn and the subsequent formation of a disulfide bond, induce conformational change to activate the channels, 2) H2Sn is produced by 3-mercaptopyruvate sulfurtransferase, 3) H2Sn react with cysteine and glutathione to produce Cys-SSH and GSSH, potential regulators of cellular redox state.

Research Products

• [Journal Article] Alternative pathway of H2S and polysilfides production form reaction intermediate of 3-mercaptopyruvate sulfurtransferase (2018)
  • Author(s): Nagahara N, Koike S, Nirasawa T ,Kimura H, Ogasawara Y
  • Journal Title: Biochem Biophys Res Commun Volume: 496 Issue: 2 Pages: 648-653
  • DOI: 10.1016/j.bbrc.2018.01.056
  • Peer Reviewed / Open Access
• [Journal Article] Discovery and Mechanistic Characterization of Selective Inhibitors of H2S-producing Enzyme: 3-Mercaptopyruvate Sulfurtransferase (3MST) Targeting Active-site Cysteine Persulfide (2017)
  • Author(s): K. Hanaoka, K. Sasakura, Y. Suwanai, S. Toma-Fukai, K. Shimamoto, Y. Takano, N. Shibuya, T. Terai, T. Komatsu, T. Ueno, Y. Ogasawara, Y. Tsuchiya, Y. Watanabe, H. Kimura, C. Wang, M. Uchiyama, H. Kojima, T. Okabe, Y. Urano, T. Shimizu, T. Nagano
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 40227-40227
  • DOI: 10.1038/srep40227
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 3-Mercaptopyruvate sulfurtransferase produces potential redox regularots cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S. (2017)
  • Author(s): Kimura, Y., Koike, S., Shibuya, N., Lefer, D., Ogasawara, Y., Kimura, H.
  • Journal Title: Sci. Rep. Volume: 7 Issue: 1 Pages: 10459-10459
  • DOI: 10.1038/s41598-017-11004-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Analysis of endogenous H2S and H2Sn in mouse brain by high-performance liquid chromatography with fluorescence and tandem mass spectrometric detection. (2017)
  • Author(s): Koike, S., Kawamura, K., Kimura, Y., Shibuya, N., Kimura, H., Ogasawara, Y.
  • Journal Title: Free Rad. Biol. Med. Volume: 113 Pages: 355-362
  • DOI: 10.1016/j.freeradbiomed.2017.10.346
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hydrogen sulfide and polysalfide signaling (2017)
  • Author(s): Kimura Hideo
  • Journal Title: Antioxid. Redox Signal. Volume: 27 Issue: 10 Pages: 619-621
  • DOI: 10.1089/ars.2017.7076
  • Peer Reviewed
• [Journal Article] Development of a Reversible Fluorescent Probe for Reactive Sulfur Species, Sulfane Sulfur, and its Biological Application (2017)
  • Author(s): Takano Y, Hanaoka K, Shimamoto K, Miyamoto R, Komatsu T, Ueno T, Terai T, Kimura H, Nagano T, Urano Y
  • Journal Title: Chem Commun Volume: 53 Issue: 6 Pages: 1064-1067
  • DOI: 10.1039/c6cc08372b
  • Peer Reviewed / Open Access
• [Journal Article] Polysulfides (H2Sn) produced from the interaction of hydrogen sulfide (H2S) and nitric oxide(NO) activate TRPA1 channels (2016)
  • Author(s): R Miyamoto, S Koike, Y Takano, N Shibuya, Y Kimura, K Hanaoka, Y Urano, Y Ogasawara,H Kimura
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 45995-45995
  • DOI: 10.1038/srep45995
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 硫化水素とポリサルファイドの生理機能 (2016)
  • Author(s): 木村英雄
  • Journal Title: 日薬理誌 Volume: 147 Pages: 23-29
  • NAID: 130005118581
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hydrogen polysulfide (H2Sn) signaling along with hydrogen sulfide (H2S) and nitric oxide (NO). (2016)
  • Author(s): Kimura, H.
  • Journal Title: J. Neural Transm Volume: 123 Issue: 11 Pages: 1235-1245
  • DOI: 10.1007/s00702-016-1600-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain. (2015)
  • Author(s): Yuka Kimura, Yukiko Toyofuku, Shin Koike, Norihiro Shibuya, Noriyuki Nagahara, David Lefer, Yuki Ogasawara, Hideo Kimura.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 14774-14774
  • DOI: 10.1038/srep14774
  • Peer Reviewed / Open Access
• [Journal Article] Hydrogen sulfide and polysulfides as signaling molecules (2015)
  • Author(s): Kimura, H.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 91 Issue: 4 Pages: 131-159
  • DOI: 10.2183/pjab.91.131
  • NAID: 130005061418
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Polysulfide promotes neuroblastoma cell differentiation by accelerating calcium influx (2015)
  • Author(s): 103. Koike, S., Shibuya, N., Kimura, H., Ishii, K., Ogasawara, Y.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 459 Issue: 3 Pages: 488-492
  • DOI: 10.1016/j.bbrc.2015.02.133
  • Peer Reviewed
• [Journal Article] H2S2014 in Kyoto: The 3rd International Conference on H2S in Biology and Medicine. (2015)
  • Author(s): Kimura, H.
  • Journal Title: Nitric Oxide Volume: 46 Pages: 1-6
  • DOI: 10.1016/j.niox.2014.10.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons. (2015)
  • Author(s): Hatakeyama Y, Takahashi K, Tominaga M, Kimura H, Ohta T.
  • Journal Title: Molecular Pain Volume: 11 Pages: 24-24
  • DOI: 10.1186/s12990-015-0023-4
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Physiological Roles of Hydrogen Sulfide and Polysulfides.Review (2015)
  • Author(s): Kimura H.
  • Journal Title: Handb Exp Pharmacol. Volume: 230 Pages: 61-80
  • DOI: 10.1007/978-3-319-18144-8_3
  • ISBN: 9783319181431, 9783319181448
  • Acknowledgement Compliant
• [Journal Article] Signaling molecules:Hydrogen sulfide and polylsulfide (2015)
  • Author(s): Hideo Kimura
  • Journal Title: Antioxidants & Redox Signaling Volume: 22 Issue: 5 Pages: 362-376
  • DOI: 10.1089/ars.2014.5869
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Signaling of Hydrogen sulfide and polysulfides (2015)
  • Author(s): Hideo Kimura
  • Journal Title: Antioxidants & Redox Signaling Volume: 22 Issue: 5 Pages: 347-349
  • DOI: 10.1089/ars.2014.6082
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hydrogen sulfide and polysulfides as biological mediators (2014)
  • Author(s): Kimura, H.
  • Journal Title: molecules Volume: 19 Issue: 10 Pages: 16146-16157
  • DOI: 10.3390/molecules191016146
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ポリサルファイドH2S3とH2Sは脳内で３－メルカプトピルビン酸イオウ転移酵素により産生される (2017)
  • Author(s): 木村由佳、豊福優希子、小池伸、渋谷典広、永原則之、David Lefer、小笠原祐樹、木村英雄
  • Organizer: 第30回日本薬理学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-15
• [Presentation] 教育セミナー、シグナル分子:硫化水素(H2S)とポリサルファイド(H2Sn) (2017)
  • Author(s): 木村英雄
  • Organizer: 第90回日本薬理学会年会
  • Place of Presentation: 長崎
  • Year and Date: 2017-03-15
  • Invited
• [Presentation] Mechanism of polysulfide production: enzymatic biosynthesis and chemical interaction between hydrogen sulfide and nitric oxide. (2017)
  • Author(s): Yuka Kimura,
  • Organizer: ConBio2017
  • Int'l Joint Research
• [Presentation] Signaling molecules: H2S and H2Sn. (2017)
  • Author(s): Kimura, H.
  • Organizer: ConBio2017
  • Int'l Joint Research
• [Presentation] 生理活性物質硫化水素とポリサルファイドの産生機構。 (2017)
  • Author(s): 渋谷典広
  • Organizer: ConBio2017
  • Int'l Joint Research
• [Presentation] Identification of H2S3 and H2S by 3-mercaptopyruvate sulfurtransferase in the mammalian brain (2016)
  • Author(s): Y Kimura, Y Toyofuku, S Koike, N Shibuya, N Nagahara, D Lefer, Y Ogasawara, H Kimura
  • Organizer: 第89回日本生化学会大会 (口演、ポスター発表）
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] 硫化水素（H2S)とポリサルファイド（H2Sn）によるシグナル伝達 (2016)
  • Author(s): 木村英雄、木村由佳、小池伸、豊福優希子、渋谷典広、小笠原祐樹、永原則之
  • Organizer: 第89回日本生化学会大会（口演、ポスター発表）
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] D-システインからの硫化水素産生 (2016)
  • Author(s): 渋谷典広、小池伸、田中真紀子、湯浅磨里、木村由佳、小笠原祐樹、福井清、永原則之、木村英雄
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] Physiological roles of hydrogen sulfide (H2S) and hydrogen polysulfide (H2Sn) (2016)
  • Author(s): H Kimura, Y Kimura, Y Toyofuku, N Shibuya, S Koike, Y Ogasawara, N Nagahara, D Lefer, Y Mikami, J-i Oka
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Identification of H2S3 as a novel signaling molecule in the brain, and its biosynthesis by 3-mercaptopyruvate sulfurtransferase (2016)
  • Author(s): Y Kimura, Y Toyofuku, S Koike, N Shibuya, N Nagahara, D Lefer, Y Ogasawara, H Kimura
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Another pathway to produce H2S (2016)
  • Author(s): N Shibuya, S Koike, M Tanaka, M Yuasa, Y Kimura, Y Ogasawara, L Fukui, N Nagahara, H Kimura
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
• [Presentation] Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain (2016)
  • Author(s): 木村由佳、豊福優希子、小池伸、渋谷典広、永原則之、David Lefer、小笠原祐樹、木村英雄
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜会議センター
  • Year and Date: 2016-03-09
• [Presentation] マウスにおける生理活性物質H2Sの産生経路 (2016)
  • Author(s): 渋谷典弘, 小池伸, 田中真紀子, 湯浅(石上)磨里, 木村由佳, 小笠原祐樹2, 福井清3, 永原則之, 木村英雄
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜会議センター
  • Year and Date: 2016-03-09
• [Presentation] 硫化水素とポリサルファイドの生理機能 (2016)
  • Author(s): 木村英雄
  • Organizer: 第89回日本薬理学会年会
  • Place of Presentation: パシフィコ横浜会議センター
  • Year and Date: 2016-03-09
• [Presentation] The signaling molecules: hydrogen sulfide (H2S) and hydrogen polysulfides (H2Sn) (2016)
  • Author(s): H Kimura
  • Organizer: A Symposium on Pharmacology of Gaseous Mediators
  • Place of Presentation: シンガポール
  • Int'l Joint Research / Invited
• [Presentation] 教育講演；ガスによる脳疾患の治療の可能性、脳と硫化水素 (2016)
  • Author(s): 木村英雄
  • Organizer: 第20回日本医療ガス学会学術大会・総会
  • Place of Presentation: 秋田
  • Invited
• [Presentation] Hydrogen sulfide (H2S) and hydrogen polysulfides (H2Sn) as signaling molecules (2016)
  • Author(s): H Kimura
  • Organizer: 9th International Conference on the Biology, Chemistry, and Therapeutic Applications of nitric Oxide
  • Place of Presentation: 仙台
  • Int'l Joint Research / Invited
• [Presentation] Production and functions of hydrogen sulfide and hydrogen polysulfides (2016)
  • Author(s): H Kimura
  • Organizer: 4th International Conference on Hydrogen sulfide in Biology and Medicine
  • Place of Presentation: イタリア、ナポリ
  • Int'l Joint Research / Invited
• [Presentation] Signaling molecules: hydrogen sulfide and polysulfides (2016)
  • Author(s): H Kimura
  • Organizer: Annual Meeting of the Society for Free Radical research Europe
  • Place of Presentation: ハンガリー、ブタベスト
  • Int'l Joint Research / Invited
• [Presentation] 脳のTRPA!チャネルを活性化するシグナル分子としてのポリサルファイドとその生合成 (2015)
  • Author(s): 三上義礼、大隅貴美子、津金麻美子、岡淳一郎、豊福優希子、小池伸、渋谷典広、永原則之、小笠原祐樹、木村英雄
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 生理活性物質硫化水素の産生経路 (2015)
  • Author(s): 渋谷典弘, 小池伸, 田中真紀子, 湯浅(石上)磨里, 木村由佳, 小笠原祐樹, 福井清, 永原則之, 木村英雄
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] シグナル分子としての硫化水素（H2S)とポリサルファイド(H2Sｎ) (2015)
  • Author(s): 木村英雄
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] 結合型イオウの一種であるポリスルフィドは神経細胞を酸化ストレスから保護し、神経突起の伸長を促進する (2015)
  • Author(s): 小池伸、渋谷典広、木村英雄、鈴木俊宏、小笠原祐樹
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] The physiological roles of hydrogen sulfide and polysulfides (2015)
  • Author(s): Kimura, H.
  • Organizer: 14th International Congress on Amino Acids, Peptides and Proteins
  • Place of Presentation: Juridicum, Vienna, Austria
  • Year and Date: 2015-08-03
  • Int'l Joint Research / Invited
• [Presentation] Polysulflides derived from H2S, are possible signaling molecules that activate TRPA1 channels in rat brain (2015)
  • Author(s): Yuka Kimura, Yoshinori Mikami, Kimiko Osumi, Mamiko Tsugane, Jun-ichiro Oka, Hideo Kimura
  • Organizer: 第38回神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2015-07-28
• [Presentation] A novel pathway for the production of hydrogen sulfide from D-cysteine in mammalian cells (2015)
  • Author(s): Norihiro Shibuya, Shin Koike, Makiko Tanaka, Mari Ishigame-Yuasa, Yuka Kimura, Yuki Ogasawara, Kiyoshi Fukui, Noriyuki Nagahara, Hideo Kimura
  • Organizer: 第38回神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2015-07-28
• [Presentation] Hydrogen sulfide and polysulfides as signaling molecules (2015)
  • Author(s): Hideo Kimura, Yuka Kimura, Norihiro Shibuya, Yukiko Toyofuku, Jun-ichiro Oka, Yuki Ogasawara, Shin Koike, Mamiko Tsugane
  • Organizer: 第38回神経科学大会
  • Place of Presentation: 神戸コンベンションセンター
  • Year and Date: 2015-07-28
• [Presentation] シグナル分子としての硫化水素とポリサルファイド (2015)
  • Author(s): 木村英雄
  • Organizer: 第15回日本NO学会学術集会
  • Place of Presentation: 大阪、千里ライフサイエンスセンター
  • Year and Date: 2015-06-26
  • Invited
• [Presentation] Hydrogen sulfide and polysulfides as signaling molecules (2015)
  • Author(s): Kimura, H.
  • Organizer: 3rd European Conference on the Biology of Hydrogen Sulfide (H2S2015)
  • Place of Presentation: Athens, Greek
  • Year and Date: 2015-05-03
  • Int'l Joint Research / Invited
• [Presentation] 活性型の硫化水素であるポリサルファイドはラット脳でTRPA1チャネルを活性化する (2015)
  • Author(s): 木村由佳、三上義礼、大隅貴美子、津金麻美子、岡淳一郎、木村英雄
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 愛知県・名古屋市・名古屋国際会議場
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] マウスにおける生理活性物質H2Sの産生経路 (2015)
  • Author(s): 渋谷典広、小池伸、 田中真紀子、湯浅(石上)磨里、木村由佳、小笠原祐樹、福井清、永原則之、木村英雄
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 愛知県・名古屋市・名古屋国際会議場
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] 硫化水素とポリサルファイドの生理機能 (2015)
  • Author(s): 木村英雄
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 愛知県・名古屋市・名古屋国際会議場
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] ポリスルフィドは侵害受容性TRPA1チャネルの活性化を介して炎症性疼痛を惹起する (2015)
  • Author(s): 畠山 由香里、高橋 賢次、富永 真琴、木村 英雄、太田 利男
  • Organizer: 第88回日本薬理学会年会
  • Place of Presentation: 愛知県・名古屋市・名古屋国際会議場
  • Year and Date: 2015-03-18 – 2015-03-20
• [Presentation] Polysulfides derived from H2S are possible signaling molecules that activate TRPA1 channels in rat brain (2014)
  • Author(s): Yuka Kimura, Yoshinori Mikami, Kimiko Osumi, Mamiko Tsugane, Jun-ichiro Oka, Hideo Kimura
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都府・京都市・国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Physiological roles of hydrogen sulfide and polysulfides (2014)
  • Author(s): Hideo Kimura, Norihiro Shibuya, Yoshinori Mikami, Yuka Kimura, Yuｋi Ogasawara, Noriyuki Nagahara, Jun-ichiro Oka, Shin Koike
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都府・京都市・国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] D-システインからの生理活性物質硫化水素の生産 (2014)
  • Author(s): 渋谷典広、小池伸、 田中真紀子、湯浅(石上)磨里、木村由佳、小笠原祐樹、福井清、永原則之、木村英雄
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 京都府・京都市・国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] Physiological role of hydrogen sulfide and polysulfides (2014)
  • Author(s): Hideo Kimura
  • Organizer: International symposium Gasotransmitters: physiology and pathophysiology
  • Place of Presentation: Kazan Medical School, Kazan, Russia
  • Year and Date: 2014-09-21 – 2014-09-23
  • Invited
• [Presentation] 硫化水素の合成調節と視神経における役割 (2014)
  • Author(s): 木村英雄、三上義礼、渋谷典広、木村由佳、小笠原祐樹、永原則之、山田雅弘
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川県・横浜市・パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 生理活性物質硫化水素の新規生産経路 (2014)
  • Author(s): 渋谷典広、小池伸、田中真紀子、湯浅(石上)磨里、木村由佳、小笠原祐樹、福井清、永原則之、木村英雄
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川県・横浜市・パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 硫化水素由来のポリサルファイドは、ラット脳でTRPA1を活性化するシグナル分子である (2014)
  • Author(s): 木村由佳、三上義礼、大隅貴美子、津金麻美子、岡淳一郎、木村英雄
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 神奈川県・横浜市・パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] The pharmacology of H2S (2014)
  • Author(s): Hideo Kimura
  • Organizer: 17th World Congress of basic and clinical Pharmacology
  • Place of Presentation: Ｉｎｔｅｒｎａｔｉｏｎａｌ Convention Center, Cape Town, South Africa
  • Year and Date: 2014-07-13 – 2014-07-18
  • Invited
• [Presentation] 生理活性物質としての硫化水素(H2S)とポリサルファイド(H2Sn) (2014)
  • Author(s): 木村英雄
  • Organizer: 第18回アミノ酸セミナー基礎と臨床を結ぶ
  • Place of Presentation: 東京都・千代田区・東京国際フォーラム
  • Year and Date: 2014-07-04
  • Invited
• [Presentation] Physiological role of hydrogen sulfide and polysulfdes (2014)
  • Author(s): Hideo Kimura
  • Organizer: Nitric Oxide-Nitrite/Nitrate Conference
  • Place of Presentation: Renaissance Cleveland Hotel, Cleveland, USA
  • Year and Date: 2014-06-16 – 2014-06-20
  • Invited
• [Presentation] Polysulfides are possible H2S-derived signaling molecules in rat brain (2014)
  • Author(s): Yuka Kimura, Yoshinori Mikami, Kimiko Osumi, Mamiko Tsugane, Jun-ichiro Oka, Hideo Kimura
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] A novel pathway for the production of hydrogen sulfide from D-cysteine in mammalian cells (2014)
  • Author(s): Norihiro Shibuya, Shin Koike, Makiko Tanaka, Mari Ishigami-Yuasa, Yuka Kimura, Yuki Ogasawara, Kiyoshi Fukui, Noriyuki Nagahara, Hideo Kimura
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Polysulifde exerts protective effect against cytotoxicity through Nrf2 signaling in neuronal cells (2014)
  • Author(s): Shin Koike, Yuri Ogasawara, Norihiro Shibuya, Kazuyuki Ishii, Hideo Kimura
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Inhibitor screening for 3-mercaptopyruvate sulfurtransferase (3MST) and cystathionine gamma lyase (CSE) (2014)
  • Author(s): Kazuhito Shimamoto, Kenjiro Hanaoka, Kiyoshi Sasakura, Yukihiro Tsuchiya, Yasuo Watanabe, Hideo Kimura, Takayoshi Okabe, Tetsuo Nagano, Yasuteru Urano
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] H2S and beyond (2014)
  • Author(s): Hideo Kimura
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Nociceptive action of hydrogen sulfide through the activation of transient receptor potential ankyrin 1 (TRPA1) (2014)
  • Author(s): Hitomi Ogawa, Yukari Hatakeyama, Kenji Takahashi, Makoto Tominaga, Hideo Kimura, Toshio Ohta
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] Bound sulfur exerts protective effect against cytotoxicity in neuronal cells (2014)
  • Author(s): Yuki Ogasawara, Shin Koike, Norihiro Shibuya, Kazuyuki Ishii, Hideo Kimura
  • Organizer: the Third International Conference on H2S Biology and Medicine
  • Place of Presentation: 京都府・京都市・芝蘭会館
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] システインから生合成される硫化水素(H2S)とその代謝産物ポリサルファイドの機能 (2014)
  • Author(s): 木村英雄
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 北海道・札幌市・酪農学園大学
  • Year and Date: 2014-05-30 – 2014-06-01
  • Invited
• [Presentation] Physiological role of hydrogen sulfide and polysulfides (2014)
  • Author(s): Hideo Kimura
  • Organizer: International symposium on: hydrogen sulphide and nitric oxide in health and disease
  • Place of Presentation: Centro Conferenze-Universita degli Studi FedericoII, Naples, Italy
  • Year and Date: 2014-05-15 – 2014-05-17
  • Invited
• [Presentation] Hydrogen sulfide:overview of its production and function (2014)
  • Author(s): Hideo Kimura
  • Organizer: Experimenta Biology 2014
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2014-04-26 – 2014-04-30
  • Invited
• [Remarks] シグナル分子硫化水素と一酸化窒素の相乗効果が ポリサルファイド（H2Sn）生成によることを解明
• [Remarks] NCNP 神経研究所 木村英雄 部長 第4回リサーチフロントアワード（トムソン・ロイター）を受賞
  • URL: http://www.ncnp.go.jp/press/release.html?no=342
• [Remarks] 木村英雄博士がトムソン・ロイター第4回リサーチフロントアワードを受賞