Elucidation of novel function of biologic defense molecule collectin CL-K1

• Project/Area Number: 26460353
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Asahikawa Medical College
• Principal Investigator: Ohtani Katsuki 旭川医科大学, 医学部, 准教授 (90396367)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: コレクチン / 発生・分化 / 糖鎖 / 自然免疫 / 補体

Outline of Final Research Achievements

Collectin CL-K1 was a secreted protein and was known as an innate immune molecule involved in elimination of microbes, but CL-K1 was involved in development and growth, so we attempted to elucidate its role and mechanism. Regarding the developmental process, phenotypic abnormalities could be found in CL-K1 KO mice from the morphology from embryogenesis to birth after implantation and from blood biochemical data. Phenotypic analysis in adults revealed abnormal tissue in H-E staining of each tissue specimen, further found abnormality in bone formation by using X-ray and CT apparatus, and clarified renal function abnormality by hematological analysis and urinalysis.
By clarifying completely new functions other than biological defense of collectins, "dual function of biological defense proteins" could be proved in animal models.

Research Products

• [Journal Article] Collectin kidney 1 plays an important role in innate immunity against Streptococcus pneumoniae infection. (2017)
  • Author(s): Hwang I, Mori K, Ohtani K, Matsuda Y, Roy N, Kim Y, Suzuki Y, Wakamiya N
  • Journal Title: J Innate Immun Volume: 9 Issue: 2 Pages: 217-228
  • DOI: 10.1159/000453316
  • NAID: 120006711670
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The collectins CL-L1, CL-K1 and CL-P1, and their roles in complement and innate immunity. (2016)
  • Author(s): Hansen SW, Ohtani K, Roy N, Wakamiya N
  • Journal Title: Immunobiology Volume: 221 Issue: 10 Pages: 1058-1067
  • DOI: 10.1016/j.imbio.2016.05.012
  • NAID: 120006325059
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis (2015)
  • Author(s): Troegeler A, Lugo-Villarino G, Hansen S, Rasolofo V, Henriksen ML, Mori K, Ohtani K, Duval C, Mercier I, Bénard A, Nigou J, Hudrisier D, Wakamiya N, Neyrolles O
  • Journal Title: PLoS One Volume: 10 Issue: 7 Pages: e0132692-e0132692
  • DOI: 10.1371/journal.pone.0132692
  • NAID: 120005828841
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular basis of sugar recognition by collectin-K1 and the effects of mutations associated with 3MC syndrome (2015)
  • Author(s): Venkatraman Girija U, Furze CM, Gingras AR, Yoshizaki T, Ohtani K, Marshall JE, Wallis AK, Schwaeble WJ, El-Mezgueldi M, Mitchell DA, Moody PC, Wakamiya N, Wallis R
  • Journal Title: BMC Biol Volume: 13 Issue: 1 Pages: 27-27
  • DOI: 10.1186/s12915-015-0136-2
  • NAID: 120005828840
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 新規コレクチンCL-K1は、マウスにおける肺炎球菌に対して防御的に働く (2016)
  • Author(s): 黄 仁秀、森 健一郎、大谷 克城、松田 泰幸、Roy Nitai、若宮 伸隆
  • Organizer: 日本生化学会
  • Place of Presentation: 仙台国際センター／東北大学川内北キャンパス（宮城県仙台市青葉区）
  • Year and Date: 2016-09-25
• [Presentation] Structural characterization of novel collectins, CL-K1, CL-L1, and CL-LK in blood (2016)
  • Author(s): Yasuyuki Matsuda, Katsuki Ohtani, Nitai Roy, Insu Hwang, Kenichiro Mori, Nobutaka Wakamiya
  • Organizer: 26th International Complement Workshop
  • Place of Presentation: ホテル日航金沢（石川県金沢市）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] In vitro and in vivo roles in Collectin Kidney 1 (CL-K1) with innate immunity against Streptococcus pneumoniae (2016)
  • Author(s): Insu Hwang, Kenichiro Mori, Katsuki Ohtani, Yasuyuki Matsuda, Nitai Roy, YounUck Kim, Nobutaka Wakamiya
  • Organizer: 26th International Complement Workshop
  • Place of Presentation: ホテル日航金沢（石川県金沢市）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] コレクチンCL-K1の糖鎖認識と3MC症候群における変異の分子機構への影響 (2015)
  • Author(s): Ohtani K, Venkatraman Girija U, Mori K, Yoshizaki T, Matsuda Y, Hwang I, Roy N, Wallis R, Wakamiya N
  • Organizer: BMB2015（第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会）
  • Place of Presentation: 神戸ポートアイランド（神戸市中央区）
  • Year and Date: 2015-12-01
• [Presentation] Molecular basis of sugar recognition by CL-K1 and the effects of mutations associated with 3MC syndrome (2015)
  • Author(s): Venkatraman Girija U, Furze CM, Gingras AR, Yoshizaki T, Ohtani K, Marshall JE, Wallis AK, Schwaeble WJ, El-Mezgueldi M, Mitchell DA, Moody PC, Wakamiya N, Wallis R
  • Organizer: GLYCO 23 XXIII International Symposium on Glycoconjugates
  • Place of Presentation: Split, Croatia
  • Year and Date: 2015-09-15
  • Int'l Joint Research
• [Presentation] CL-K1は、肺炎球菌感染に対し、補体レクチン経路を介して生体防御に機能する (2015)
  • Author(s): 黄 仁秀、森 健一郎、松田 泰幸、ロイ ニタイ、大谷 克城、若宮 伸隆
  • Organizer: 日本糖質学会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-07-31
• [Presentation] 新規コレクチンCL-K1と相互作用する血漿由来タンパク質の同定 (2015)
  • Author(s): 松田 泰幸、大谷 克城、森 健一郎、ロイ ニタイ、黄 仁秀、若宮 伸隆
  • Organizer: 日本糖質学会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-07-31
• [Presentation] コレクチンCL-K1の生化学的解析 (2015)
  • Author(s): 大谷 克城、Venkatraman Girija U、森 健一郎、吉崎 隆之、松田 泰幸、黄 仁秀、ロイ ニタイ、Wallis R、若宮 伸隆
  • Organizer: 日本糖質学会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-07-31
• [Presentation] コレクチンCL-K1の凝固系への関与について (2014)
  • Author(s): 大谷 克城、Kazue Takahashi、早川 峰司、森 健一郎、黄 仁秀、吉崎 隆之、松田 泰幸、ロイ ニタイ、丸藤 哲、若宮 伸隆
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館（京都市左京区）
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] The new biological aspects in CL-K1 (Collectin Kidney 1) (2014)
  • Author(s): Kazue Takahashi, Katsuki Ohtani, Mykol Larvie, Patience Moyo, Lorencia Chigweshe, Elizabeth M. Van Cott, Nobutaka Wakamiya
  • Organizer: International Complement Workshop
  • Place of Presentation: Rio de Janeiro (Brazil)
  • Year and Date: 2014-09-14 – 2014-09-18
• [Presentation] コレクチンCL-K1の生体防御機能に関する検討 (2014)
  • Author(s): 黄 仁秀、森 健一郎、松田 泰幸、ロイ ニタイ、大谷 克城、若宮 伸隆
  • Organizer: 補体研究会
  • Place of Presentation: 神戸常盤大学（神戸市長田区）
  • Year and Date: 2014-08-22 – 2014-08-23
• [Presentation] コレクチンCL-K1の生化学的解析 (2014)
  • Author(s): 大谷 克城、森 健一郎、黄 仁秀、吉崎 隆之、松田 泰幸、ロイ ニタイ、若宮 伸隆
  • Organizer: 日本糖質学会
  • Place of Presentation: 名古屋大学（名古屋市千種区）
  • Year and Date: 2014-08-10 – 2014-08-12