Regulation of tissue morphogenesis and pathology by miR-199a/214
| Project/Area Number |
26460357
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Uchijima Yasunobu 東京大学, 大学院医学系研究科(医学部), 助手 (90272426)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | micro RNA / miR-199a / miR-214 / 形態形成 / 心筋線維化 / miR-199 / dnm3os / ドキソルビシン / microRNA / Dnm3os / 組織形成 / 骨形成 / 心筋傷害 / 単一細胞発現解析 |
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| Outline of Final Research Achievements |
In Dnm3os Knock-out mice, Nrip1 mRNA was increased and this gene was considered as a candidate for the target of miR-214. In vitro analysis suggested Nrip1 may contribute to the phenotypes such as decreased size observed in the KO mice. Doxorubicin (Dox) induced myocardial injury and this was suppressed by knocking in miR-199a/214 into the Ednra locus. Because the participation of neural crest cell (NCC) was suggested for the rescue mechanism of myocardium injury, NCC derived embryonic heart cells were collected and subjected to the single cell transcriptome analysis. In these cells, some expressed several smooth muscle cell markers, and a few cells expressed progenitor cell markers such as Kit, Sox10, and Pax3. The latter cells were expected to contribute to the protection from myocardium injury.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Distinct effects of Hoxa2 overexpression in cranial neural crest populations reveal that the mammalian hyomandibular-ceratohyal boundary maps within the styloid process.2015
Author(s)
Kitazawa T, Fujisawa K, Narboux-Neme N, Arima Y, Kawamura Y, Inoue T, Wada Y, Kohro T, Aburatani H, Kodama T, Kim KS, Sato T, Uchijima Y, Maeda K, Miyagawa-Tomita S, Minoux M, Rijli FM, Levi G, Kurihara Y, Kurihara H
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Journal Title
Developmental Biology
Volume: 15
Issue: 2
Pages: 162-174
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Homeotic transformation of the upper jaw into lower jaw-like structures in Dlx5-overexpressing mice2014
Author(s)
Shimizu, M, Kitazawa, T, Kawamura, Y, Narboux-Neme, N, Levi G, Uchijima Y, Kurihara Y, Kurihara H
Organizer
第47回日本発生生物学会
Place of Presentation
愛知県名古屋市WINC AICHI
Year and Date
2014-05-28 – 2014-05-29
Related Report
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