Analysis of tumor suppressors RASSF3 and RASSF6

• Project/Area Number: 26460359
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Iwasa Hiroaki 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (70582188)
• Co-Investigator(Renkei-kenkyūsha): Maruyama Junichi 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (30723639)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: シグナル伝達 / 腫瘍抑制 / フェイルセーフ / RASSF / 翻訳後修飾

Outline of Final Research Achievements

Ras association domain family (RASSF) cooperates with tumor suppressors. The RASSF tumor suppressor genes are frequently inactivated in many types of human cancer. In this study we found that a novel tumor suppressor acts through RASSF. We also found that a cellular precursor state of tumor changes the structure of RASS protein to suppress tumor formation. Thus we propose that RASSF is a primary fail-safe mechanism of oncogene-driven tumorigenesis.

Research Products

• [Journal Article] Domain analysis of Ras-association domain family member 6 upon interaction with MDM2. (2017)
  • Author(s): Sarkar A, Iwasa H, Hossain S, Xu X, Sawada T, Shimizu T, Maruyama J, Arimoto-Matsuzaki K, Hata Y.
  • Journal Title: FEBS Letters Volume: 591 Issue: 2 Pages: 260-272
  • DOI: 10.1002/1873-3468.12551
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Validation of the chemical compound library screening for TAZ inhibitors by use of green fluorescence protein-fused TAZ. (2016)
  • Author(s): Nagashima S, Maruyama J, Kawano S, Iwasa H, Nakagawa K, Ishigami-Yuasa M, Kagechika H, Nishina H, Hata Y.
  • Journal Title: Cancer Sci. Volume: - Issue: 6 Pages: 791-802
  • DOI: 10.1111/cas.12936
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. (2015)
  • Author(s): Kawano S, Maruyama J, Nagashima S, Inami K, Qiu W, Iwasa H, Nakagawa K, Ishigami-Yuasa M, Kagechika H, Nishina H, Hata Y.
  • Journal Title: J. Biochem. Volume: 158 Issue: 5 Pages: 413-423
  • DOI: 10.1093/jb/mvv051
  • NAID: 40020646985
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] RASSF6; the Putative Tumor Suppressor of the RASSF Family. (2015)
  • Author(s): Iwasa H, Jiang X, Hata Y.
  • Journal Title: Cancers (Basel) Volume: 9 Issue: 4 Pages: 2415-2426
  • DOI: 10.3390/cancers7040899
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] MAGI2/S-SCAM outside brain (2015)
  • Author(s): Nagashima S, Kodaka M, Iwasa H, Hata Y.
  • Journal Title: Journa of Biochemistry Volume: 157 Issue: 4 Pages: 177-184
  • DOI: 10.1093/jb/mvv009
  • NAID: 40020428694
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] UNC119Aは腫瘍抑制分子RASSF6と共にDNA損傷応答を制御する (2015)
  • Author(s): 岩佐宏晃、清水尊仁、畑裕
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド（兵庫県、神戸市）
  • Year and Date: 2015-12-01
• [Book] 別冊・医学のあゆみ 広がるHippo pathway研究-癌から各種疾患へ (2015)
  • Author(s): 岩佐宏晃（共著）
  • Publisher: 医歯薬出版株式会社