| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Zfp296 has been implicated in stem cell pluripotency and tumor pathogenesis. However, its mechanisms remain elusive. Here, we demonstrated that a Zfp296 deficiency in mice impairs germ-cell development and embryonic growth. Zfp296 was intracellularly localized to heterochromatin in embryos. In addition, we showed that Zfp296 interacts with epigenetic regulator proteins in vitro. We observed that the H3K9 methylation levels in Zfp296-/- embryonic cells increase and that Zfp296 deficiency affects the H3K9me3 levels at major satellite repeats. Our results demonstrate that Zfp296 is a component of constitutive heterochromatin that affects embryonic development by negatively regulating H3K9 methylation.
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