| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
We generated a structural model of JRAB/MICAL-L2 through a combination of bioinformatic and biochemical analyses and thereby revealed how JRAB/MICAL-L2 interplays with Rab13 and how its conformational change occurs. Moreover, we elucidated that the conformational plasticity in JRAB/MICAL-L2 causes spatiotemporal actin cytoskeletal rearrangement to control direction and speed of cell movement and traction force of the cell through a robust approach combining cell biology, live imaging, computational biology, and biomechanics. Thus, our multidisciplinary approach brings a novel concept that the conformational plasticity of a single molecule provides dynamics in higher cellular function. These findings will be of help to understand the dynamics in higher structure and function of brain.
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