in vivo analysis of Mesenchymal stem cells using Runx2 conditional KO mouse
| Project/Area Number |
26460387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Okayama University (2016)
Kanazawa University (2014-2015) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Hinoi Eiichi 金沢大学, 薬学系, 准教授 (70360865)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 間葉系幹細胞 / Runx2 / 転写制御因子 |
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| Outline of Final Research Achievements |
The cellular origin and essential period for Runx2 function during osteoblast differentiation in intramembranous ossification remain poorly understood. Paired related homeobox 1 (Prx1) is expressed in craniofacial mesenchyme, and Runx2 deficiency in Prx1+-derived cells (Runx2prx1－/－ mice) resulted in defective intramembranous ossification. Double-positive cells for Prx1-GFP and stem cell antigen-1 (Sca1) (Prx1+Sca1+ cells) in the calvaria expressed Runx2 at lower levels and were more homogeneous and primitive as compared with Prx1+Sca1－ cells. These findings indicate that the essential period of Runx2 function on intramembranous ossification would begin at the Prx1+Sca1+ mesenchymal stem cell stage and end at the Osx+Prx1－Sca1－ osteoblast precursor stage.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] ATF3 controls proliferation of osteoclast precursor and bone remodeling.2016
Author(s)
Fukasawa K, Park G, Iezaki T, Horie T, Kanayama T, Ozaki K, Onishi Y, Takahata Y, Yoneda Y, Takarada T, Kitajima S, Vacher J, Hinoi E.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 30918-30918
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Transcriptional Modulator Ifrd1 Regulates Osteoclast Differentiation through Enhancing the NF-κB/NFATc1 Pathway.2016
Author(s)
Iezaki T, Fukasawa K, Park G, Horie T, Kanayama T, Ozaki K, Onishi Y, Takahata Y, Nakamura Y, Takarada T, Yoneda Y, Nakamura T, Vacher J, Hinoi E.
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Journal Title
Mol Cell Biol.
Volume: 36(19)
Issue: 19
Pages: 2451-2463
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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