| Project/Area Number |
26460395
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Nakano Masakazu 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (70381944)
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| Co-Investigator(Kenkyū-buntansha) |
田代 啓 京都府立医科大学, 医学(系)研究科(研究院), 教授 (10263097)
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| Co-Investigator(Renkei-kenkyūsha) |
KINOSHITA SHIGERU 京都府立医科大学, 大学院医学研究科, 教授 (30116024)
SATO RYUICHI 京都府立医科大学, 大学院医学研究科, 助教 (30717533)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 血管新生 / 網膜 / 網羅的遺伝子発現解析 / マイクロアレイ / シグナル伝達 / 血管内皮細胞 / ノックダウン / 細胞接着 / 三次元培養 / 網羅的発現解析 |
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| Outline of Final Research Achievements |
As angiogenesis of mouse retina begins at right after birth, developing mouse retina has been serving as an ideal model for investigating angiogenesis. We previously performed a comprehensive gene expression analysis using pre- and postnatal mouse retina, and obtained several candidate genes that were differentially expressed during retinal development.
We here analyzed whether these genes were functionally involved in the angiogenesis. After confirming the expression results by our highly sensitive qPCR system specialized for developmental retina (Adachi et al., Genes Cells, 2015), we analyzed the effect on angiogenesis of one gene encoding a cell adhesion molecule. When the expression of this gene was transiently suppressed in mouse endothelial cells, we found that the induction of angiogenesis was significantly inhibited.
The angiogenesis-related gene identified in this study should be a promising tool for discovering a novel molecular mechanism of angiogenesis.
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