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Elucidation of the molecular mechanisms of neutrophil extracellular traps (NETs) formation

Research Project

Project/Area Number 26460405
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pathological medical chemistry
Research InstitutionHimeji Dokkyo University

Principal Investigator

Tohyama Yumi  姫路獨協大学, 薬学部, 教授 (70362770)

Co-Investigator(Renkei-kenkyūsha) TABATA Hiroyuki  姫路獨協大学, 薬学部, 講師 (70378785)
MORITA Hiroyuki  姫路獨協大学, 薬学部, 助手 (90648594)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords好中球 / NETs
Outline of Final Research Achievements

To elucidate the molecular mechanisms of neutrophil extracellular traps (NETs) formation, human neutrophils were treated with PMA and induced to form NETs in vitro. As a result of live cell imaging, the process was classified into 3 stages: stage 1: most of the cells lost lobulated nuclei; stage 2: chromatin decondensation occurred like a spherical form; stage 3: decondensed chromatin became diffuse and spread like a cloud showing NET-like structures. Next, comprehensive mass spectrometric protein analysis using iTRAQ-labeling was performed using the lysates from each stage and we found about 80 peptides (fragments of 15 proteins) containing post translational modification, which were markedly increased according to NETs formation. Among them, we focus on two proteins and are studying the effects of gene defect of these proteins in NETs formation.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (18 results) Remarks (2 results)

  • [Journal Article] Withaferin A suppresses the growth of myelodysplasia and leukemia cell lines by inhibiting cell cycle progression.2016

    • Author(s)
      Okamoto S, Tsujioka T, Suemori SI, Kida JI, Kondo T, Tohyama Y, Tohyama K
    • Journal Title

      Cancer Sci

      Volume: 107 Issue: 9 Pages: 1302-1314

    • DOI

      10.1111/cas.12988

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Five-aza-2'-deoxycytidine-induced hypomethylation of cholesterol 25-hydroxylase gene is responsible for cell death of myelodysplasia/leukemia cells.2015

    • Author(s)
      Tsujioka T, Yokoi A, Itano Y, Takahashi K, Ouchida M, Okamoto S, Kondo T, Suemori S, Tohyama Y, Tohyama K
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 16709-16709

    • DOI

      10.1038/srep16709

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Salbutamol inhibits ubiquitin-mediated survival motor neuron protein degradation in spinal muscular atrophy cells.2015

    • Author(s)
      Harahap NI, Nurputra DK, Rochmah MA, Shima A, Morisada N, Takarada T, Takeuchi A, Tohyama Y Yanagisawa S, Nishio H
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 4 Pages: 351-356

    • DOI

      10.1016/j.bbrep.2015.10.012

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suppressive effect of membrane-permeable peptides derived from autophosphorylation sites of the IGF-1 receptor on breast cancer cells.2015

    • Author(s)
      Kuroda Y, Kato-Kogoe N, Tasaki E, Yuasa-Sunagawa M, Yamanegi K, Nakasyo K, Nakase I, Futaki S, Tohyama Y, Hirose M
    • Journal Title

      European Journal of Pharmacology

      Volume: 765 Pages: 24-33

    • DOI

      10.1016/j.ejphar.2015.08.004

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] 白血球細胞株,HL60におけるKIF 20A(Kinesin family member 20A)の機能の検討2016

    • Author(s)
      有馬太陽、森田寛之、松本隆太郎、田畑裕幸、通山由美
    • Organizer
      第66回日本薬学会近畿支部大会
    • Place of Presentation
      大阪薬科大学
    • Year and Date
      2016-10-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脊髄性筋萎縮症(SMA)原因遺伝子,SMNのヒト神経細胞モデルにおける機能の解析2016

    • Author(s)
      佐々木彪曜、島亜衣、森田寛之、田畑裕幸、西尾久英、通山由美
    • Organizer
      第66回日本薬学会近畿支部大会
    • Place of Presentation
      大阪薬科大学
    • Year and Date
      2016-10-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞分化および増殖におけるKinesin family member 20A (KIF20A) の機能の検討2016

    • Author(s)
      森田寛之、有馬太陽、佐々木彪曜 、松本隆太郎、田畑裕幸、通山由美
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] KIF 20A( Kinesin family member 20A )の分化と細胞分裂への影響2016

    • Author(s)
      森田寛之、有馬太陽、佐々木彪曜、松本隆太郎、田畑裕幸、通山由美
    • Organizer
      第63回日本生化学会 近畿支部例会
    • Place of Presentation
      神戸薬科大学
    • Year and Date
      2016-05-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Staphylococcus aureusの温度刺激による抵抗性の変化2016

    • Author(s)
      川井眞好、通山由美
    • Organizer
      第63回日本生化学会 近畿支部例会
    • Place of Presentation
      神戸薬科大学
    • Year and Date
      2016-05-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脊髄性筋萎縮症 (SMA) のヒト神経細胞モデル作製と表現型の解析2016

    • Author(s)
      島亜衣、森田寛之、西尾久英、通山由美
    • Organizer
      第63回日本生化学会 近畿支部例会
    • Place of Presentation
      神戸薬科大学
    • Year and Date
      2016-05-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Kinesin family member 20A(KIF20A)の細胞増殖と細胞分化における機能の検討2015

    • Author(s)
      森田寛之,福井彩乃,小山可奈子,有馬太陽,田畑裕幸,通山由美
    • Organizer
      第88回日本生化学会大会
    • Place of Presentation
      神戸 ポートピアホテル
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Kinesin family member 20A(KIF20A)の細胞分裂と細胞分化における機能の検討2015

    • Author(s)
      福井彩乃,森田寛之,小山可奈子,有馬太陽,田畑裕幸,通山由美
    • Organizer
      第65回日本薬学会近畿支部大会
    • Place of Presentation
      大阪大谷大学
    • Year and Date
      2015-10-17
    • Related Report
      2015 Research-status Report
  • [Presentation] Kinesin family member 20A(KIF20A)の多核化と細胞分裂への関与2015

    • Author(s)
      森田寛之,福井彩乃, 小山可奈子, 有馬太陽, 通山由美
    • Organizer
      第62回日本生化学会近畿支部例会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Year and Date
      2015-05-16
    • Related Report
      2015 Research-status Report
  • [Presentation] Staphylococcus aureusの好中球に対する抵抗性の変化2015

    • Author(s)
      川井眞好,通山由美
    • Organizer
      第62回日本生化学会近畿支部例会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Year and Date
      2015-05-16
    • Related Report
      2015 Research-status Report
  • [Presentation] Role of Rab27a in highly-reactive oxygen species-related neutrophil extracellular traps (NETs) formation2014

    • Author(s)
      Tohyama Yumi, Tohyama Kaoru
    • Organizer
      第43回日本免疫学会学術集会
    • Place of Presentation
      国立京都国際会館 (京都)
    • Year and Date
      2014-12-11
    • Related Report
      2014 Research-status Report
  • [Presentation] HDAC6 may be a new target of SMN downstream regulation in the microtubule dynamics of SMA fibroblasts2014

    • Author(s)
      Dian Kesumapramudya Nurputra, Hiroyuki Morita, Hisahide Nishio, Yumi Tohyama
    • Organizer
      日本人類遺伝学会第59回大会
    • Place of Presentation
      タワーホール船堀 (東京)
    • Year and Date
      2014-11-20
    • Related Report
      2014 Research-status Report
  • [Presentation] Essential role of Rab27a in the formation of neutrophil extracellular traps (NETs)2014

    • Author(s)
      Tohyama Yumi, Kawakami Tatsumi, Morita Hiroyuki Suemori Shin-ichiro Tohyama Kaoru
    • Organizer
      第76回日本血液学会学術集会
    • Place of Presentation
      大阪国際会議場 (大阪)
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] 補体依存性食作用における中間径フィラメント,ビメンチンの機能の解析(好中球様に分化したヒト白血病細胞株、HL60による検討2014

    • Author(s)
      森田寛之,山口博文,岡本秀一郎,通山由美
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      国立京都国際会館 (京都)
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 好中球様分化プロセスにおけるPKCδの機能解析2014

    • Author(s)
      柳原弘明、本岡亜弥子、山口博文、 森田寛之、通山由美
    • Organizer
      第64回日本薬学会近畿支部大会
    • Place of Presentation
      京都薬科大学 (京都)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 補体を介した食作用における中間径フィラメント,ビメンチンの機能解析2014

    • Author(s)
      山口博文、綾部圭一郎、森田寛之、通山由美
    • Organizer
      第64回日本薬学会近畿支部大会
    • Place of Presentation
      京都薬科大学 (京都)
    • Year and Date
      2014-10-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 好中球様に分化したヒト白血病細胞株HL60におけるビメンチンの機能の検討2014

    • Author(s)
      山口博文,森田寛之,綾部圭一郎,岡本秀一郎,通山由美
    • Organizer
      第61回日本生化学会近畿支部例会
    • Place of Presentation
      京都産業大学 (京都)
    • Year and Date
      2014-05-17
    • Related Report
      2014 Research-status Report
  • [Presentation] SMN is essential for the HDAC6 mediated tubulin-deacetylation in fibroblast2014

    • Author(s)
      Dian Kesumapramudya Nurputra,Hiroyuki Morita,Hisahide Nishio,Yumi Tohyama
    • Organizer
      第61回日本生化学会近畿支部例会
    • Place of Presentation
      京都産業大学 (京都)
    • Year and Date
      2014-05-17
    • Related Report
      2014 Research-status Report
  • [Remarks] 姫路獨協大学 薬学部 医療薬学科  生化学研究室

    • URL

      http://www.himeji-du.ac.jp/faculty/dp_pharm/pharm/pt2/

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
  • [Remarks] 姫路獨協大学 薬学部 医療薬学科 生化学研究室

    • URL

      http://www.himeji-du.ac.jp/faculty/dp_pharm/pharm/pt2/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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