| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
To elucidate the molecular mechanisms of neutrophil extracellular traps (NETs) formation, human neutrophils were treated with PMA and induced to form NETs in vitro. As a result of live cell imaging, the process was classified into 3 stages: stage 1: most of the cells lost lobulated nuclei; stage 2: chromatin decondensation occurred like a spherical form; stage 3: decondensed chromatin became diffuse and spread like a cloud showing NET-like structures. Next, comprehensive mass spectrometric protein analysis using iTRAQ-labeling was performed using the lysates from each stage and we found about 80 peptides (fragments of 15 proteins) containing post translational modification, which were markedly increased according to NETs formation. Among them, we focus on two proteins and are studying the effects of gene defect of these proteins in NETs formation.
|
