| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
To clarify whether Integrin β4(β4)/ErbB2/c-Met signaling work to increase the self renewal of human prostate cancer stem cells as same as the mechanism of the signaling of mouse, we performed sphere formation assay with human prostate cancer cell which shows some abilities as stem cells. β4 knock out (KO), inhibitors of EGFR/ErbB2 or Met signaling induced a decrease of sphere formation in dose dependent manner. DNA microarray of β4-Control and β4-KO cells with activation of ErbB2/c-Met signaling by each ligand revealed that Met activation induced the increased expression of the signaling pathway regulating pluripotency of stem cells and β4 is necessary for this increase. These results indicate that β4 influences on human prostate stem cells as same as on those of mouse.
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