| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We investigated genotype-phenotype correlation in rare tumors, mainly those with FLCN germline mutations, using surgically resected specimens.
Approximately 40% of tumors with FLCN germline mutations had second hits or loss of heterozygosities. Those without second hits also demonstrated suppressed expression of FLCN at protein level, indicating that mutant FLCN had dominant-negative effects.
We successfully established some primary culture tumor cells from surgically resected renal tumors harboring FLCN germline mutations. We also established one immortalized cells using SV40T antigen.
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