| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We examined 37 consecutive cases of papillary urothelial neoplasm of the urinary bladder, from which 26 (70.3%) were suitable for karyotype analysis. In the 26, karyotype showed association of diploidy and aneuploidy in 7 tumors by Q-band analysis (61-88 yr). We could measure telomere length in the 7 tumors (total 91 metaphase spreads) by Q-FISH with a software, TFL-Telo version 2. In the Q-FISH samples from the 7 tumors, one tumor showed aneuploidy, alone. In 6 of the 7 tumors, karyotypes with diploidy and aneuploidy were mixed in the same cases. Mean telomere length of diploidy karyotype in each case was significantly longer than that of aneuploidy. Anaphase bridge (AB) analysis revealed that cases with prominent aneuploidy (more than 60 chromosomes) had more frequently AB than those with less than 50. Our data suggest that critically shortened telomeres cause chromosomal instability (aneuploidy) during progression of papillary urothelial neoplasms.
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