| Project/Area Number |
26460432
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SAWABE Motoji 東京医科歯科大学, 大学院保健衛生学研究科, 教授 (30196331)
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| Co-Investigator(Kenkyū-buntansha) |
三浦 ゆり 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究副部長 (00216574)
津元 裕樹 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (00409385)
副島 友莉恵 東京医科歯科大学, 大学院保健衛生学研究科, 助教 (60596964)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 動脈 / 老化 / プロテオーム / 動脈解離症 |
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| Outline of Final Research Achievements |
Previous studies reported that several proteins produced in vascular smooth muscle cells altered with aging, resulting in the deterioration of vascular function. We performed a proteomic study on the human aortic media with chronological aging, using autopsy samples of the aortic media from elderly, middle-aged, and young subjects. Two proteomic approaches, 2D-DIGE and iTRAQ, were performed. Differentially expressed proteins with aging were categorized into three groups: actin family proteins, extracellular matrix proteins, and antioxidant proteins. A decrease in actin and increase in other actin-related proteins in elderly media were found. The immunohistochemical analysis showed that expressions of antioxidant proteins and oxidation stress-marker proteins were higher in the elderly than in the young. In conclusions, the imbalance between actin and actin-related proteins occur with human aortic aging, and antioxidant proteins were induced by elevations in oxidative stress with aging.
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