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Analysis of pathophysiology of intratumoral microenvironments in glioblastoma using spheroid culture and histopathology

Research Project

Project/Area Number 26460434
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionYamaguchi University

Principal Investigator

KIMURA Tokuhiro  山口大学, 医学(系)研究科(研究院), 講師 (40445200)

Co-Investigator(Kenkyū-buntansha) 池田 栄二  山口大学, 医学(系)研究科(研究院), 教授 (30232177)
石井 文彩  山口大学, 医学(系)研究科(研究院), 助教 (50634747)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords病理学 / 癌 / 細胞・組織 / 脳神経疾患 / 脳腫瘍
Outline of Final Research Achievements

Glioblastoma is one of the most intractable neoplasms of the brain, and understanding its pathophysiology is important. Although the presence of necrosis is characteristic of glioblastoma, the in vitro experimental methods for modeling intratumoral microenvironment around necrosis have not been established. In this study, we established the method for culturing human glioblastoma cell lines as spheroids (cell clusters), and compared their histological findings with those of glioblastoma tissues. The area of cell death resembling necrotic foci of glioblastoma tissues emerged at the center of the spheroids. The cell death was found to be based on hypoxia and glucose shortage. We also found that PSF1, a protein related to stem-cell functions, was down-regulated by hypoxia and glucose shortage in glioblastoma cells.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (5 results)

All 2016 2015 2014 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Histological Characterization of the Tumorigenic "Peri-Necrotic Niche" Harboring Quiescent Stem-Like Tumor Cells in Glioblastoma.2016

    • Author(s)
      Ishii A, Kimura T, Sadahiro H, Kawano H, Takubo K, Suzuki M, Ikeda E
    • Journal Title

      PLoS ONE

      Volume: 11 Issue: 1 Pages: e0147366-e0147366

    • DOI

      10.1371/journal.pone.0147366

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Glioblastoma細胞におけるGINS複合体構成タンパク質の発現調節2016

    • Author(s)
      木村徳宏, 石井文彩, 高倉伸幸, 河野裕夫, 池田栄二
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台国際センター(宮城県)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] HIF-1α陽性G0期腫瘍幹細胞からみた膠芽腫の腫瘍内微小環境2015

    • Author(s)
      石井文彩、木村徳宏、貞廣浩和、河野裕夫、田久保圭誉、鈴木倫保、池田栄二
    • Organizer
      第33回日本脳腫瘍病理学会
    • Place of Presentation
      JRホテルクレメント高松(香川県)
    • Year and Date
      2015-05-29
    • Related Report
      2015 Research-status Report
  • [Presentation] 低酸素と腫瘍幹細胞―腫瘍切除標本からの考察2014

    • Author(s)
      木村徳宏、石井文彩、池田栄二
    • Organizer
      第103回日本病理学会総会
    • Place of Presentation
      広島国際会議場・ANAクラウンプラザホテル広島(広島県)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 山口大学大学院医学系研究科病理形態学

    • URL

      http://ds22.cc.yamaguchi-u.ac.jp/~pathol1/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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