Involvement of Akt/mTOR in lung cancer and design of novel therapy targeting them
| Project/Area Number | 26460438 |
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Human pathology
|
|---|
| Research Institution | Jichi Medical University |
|---|
Principal Investigator |
DOBASHI Yoh 自治医科大学, 医学部, 准教授 (90231456)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
北川 雅敏 浜松医科大学, 医学部, 教授 (50294971)
坪地 宏嘉 自治医科大学, 医学部, 教授 (50406055)
後藤 明輝 秋田大学, 医学(系)研究科(研究院), 教授 (90317090)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
OOI Akishi 金沢大学, 医学部, 教授 (50160411)
SUGIMURA Haruhiko 浜松医科大学, 医学部, 教授 (00196742)
MATSUBARA Hirochika 山梨大学, 医学部, 講師 (00374166)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed(Fiscal Year 2017)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost : ¥4,000,000、Indirect Cost : ¥1,200,000)
Fiscal Year 2016 : ¥1,170,000 (Direct Cost : ¥900,000、Indirect Cost : ¥270,000)
Fiscal Year 2015 : ¥1,690,000 (Direct Cost : ¥1,300,000、Indirect Cost : ¥390,000)
Fiscal Year 2014 : ¥2,340,000 (Direct Cost : ¥1,800,000、Indirect Cost : ¥540,000)
|
|---|
| Keywords | 肺癌 / Akt / 遺伝子増幅 / microRNA / p27 / MLPA / Ubiquitin ligase / 細胞周期制御因子 / ubiquitin ligase / Pirh2 / 遺伝子多型 |
|---|
| Outline of Final Research Achievements |
We analyzed the involvement of Akt in lung carcinomas. Results were, i) Microarray analysis revealed 28 miRNAs upregulated in AKT1 or AKT2-amplified carcinomas, including miR-200. Carcinomas with lymph vessel invasion had lower expression of miR-200a/b and in adenocarcinoma and in the early carcinomas, miR-200a was higher in AKT2-amplified group. MiR-200a was significantly correlated with the expression of its target, EphA. ii) Expressions of p27 and ubiquitin ligase Skp2, KPC and Pirh2 were analyzed. Cytoplasmic p27 was correlated with nodal metastasis and, inverse correlation between nuclear-p27 and Pirh2 was observed. Moreover, Pirh2 was correlated with Stage and overall survival. iii) We performed multiplex ligation-dependent probe amplification (MLPA) analysis with fluorescence in situ hybridization analysis and immunohistochemistry. By placing modified cutoff values, cases containing fewer cancer cells with gene increase could be picked up by MLPA using custom-made probes.
|
Report
(5results)
Research Products
(32results)
-
-
[Journal Article] Loss of YAP1 Defines Neuroendocrine Differentiation of Lung Tumors2017
Author(s)
Ito T, Matsubara D, Tanaka I, Makiya K, Tanei Z, Kumagai Y, Shiu SJ, Nakaoka H, Ishikawa S, Isagawa T, Morikawa T, Shinozaki A, Goto Y, Nakano T, Tsuchiya T, Tsubochi H, Komura D, Aburatani H, Dobashi Y, Nakajima J, Endo S, Fukayama M, Sekido Y, Niki T and Murakami Y
-
Journal Title
Can Sci
Volume : 107
Pages : 1527-38
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
-
-
[Journal Article] A case of severe uterine bleeding postpartum following manual removal of placenta increta.2016
Author(s)
Chikazawa, K., Akashi, K., Gomi, Y., Tachibana, K., Horiuchi, I., Dobashi, Y., Okochi, T., Hamamoto, K., Tanno, K. and Takagi K.
-
Journal Title
Taiwan J Obst. Gynecol.
Volume : 55
Pages : 913-4
DOI
Related Report
Peer Reviewed
-
-
-
[Journal Article] Association between AKT1 gene polymorphism rs2498794 and smoking-related traits with reference to cancer susceptibility2015
Author(s)
Nishizawa, D., Kasai, S., Hasegawa, J., Sato，N., Tanioka, F., Sugimura, H., Ikeda, K. and Dobashi, Y.
-
Journal Title
Biomedical Research. International;
Volume : 2015.94.
Pages : 316829-316829
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] Epidemiological and pathobiological profiles of clostridium perfringens infections: review of consecutive series of 33 cases over a 13-year period.2015
Author(s)
Shindo, Y., Dobashi, Y., Sakai, T., Monma, C., Miyatani, H., Yoshida, Y.
-
Journal Title
Int J Clin Exp Pathol.
Volume : 8
Pages : 569-77
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
[Journal Article] Clonal profiling of mixed lobular and ductal carcinoma revealed by multiplex ligation-dependent probe amplification and fluorescence in situ hybridization.2014
Author(s)
Tajiri, R., Inokuchi, M., Sawada-Kitamura, S., Kawashima, H., Nakamura, R., Oyama, T., Dobashi, Y., Ooi, A.
-
Journal Title
Pathol. Int.
Volume : 64
Pages : 231-6
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Solitary fibrous tumor of the thymus with variegated epithelial components.2014
Author(s)
Tsubochi, H., Endo, T., Sogabe, M., Endo, S., Morinaga, S. and Dobashi, Y.
-
Journal Title
Int J Clin Exp Pathol.
Volume : 7
Pages : 7477-84
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
