Roles of the newly identified GLI1-CXCR4 signaling in synovial sarcoma cells.

• Project/Area Number: 26460443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Aichi Medical University
• Principal Investigator: Inaguma Shingo 愛知医科大学, 医学部, 講師 (80410786)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: GLI1 / CXCR4 / 滑膜肉腫 / 転移 / 軟部腫瘍 / ヘッジホッグ / 転写因子

Outline of Final Research Achievements

In the present study, we aimed to uncover the importance of Hedgehog-related transcription factor GLI1 in synovial sarcoma, one of the highly malignant soft tissue tumors. We found that GLI1 accelerates cellular proliferation, migration, and invasion through newly identified transcriptional target gene CXCR4. In mouse lung metastasis model, GLI1 enhanced lung metastasis of synovial sarcoma cells.
We also tried to identify diagnostic marker for synovial sarcoma, based on the information of potential transcriptional target genes identified from cDNA microarray analyses performed using GLI1 or SYT-SSX1 fusion gene transfected normal mesenchymal cells. However, no specific immunohistochemcal marker was found.