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Development of the stainier and SPECT probe for the diagnosis of CXCR4 expression in small cell lung carcinoma

Research Project

Project/Area Number 26460453
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionKyoto Pharmaceutical University (2015-2016)
Kumamoto University (2014)

Principal Investigator

Hasegawa Koki  京都薬科大学, 薬学部, 准教授 (50525798)

Co-Investigator(Kenkyū-buntansha) 伊藤 隆明  熊本大学, 大学院生命科学研究部(医), 教授 (70168392)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords染色剤 / イメージング / ペプチド / 診断薬 / SPECT / Ga-67 / 肺癌 / 放射性薬剤 / CXCR4 / Kisspeptin / 肺小細胞癌 / 甲状腺髄様癌 / SPECT/CT / 転移
Outline of Final Research Achievements

We have been focusing on Kiss1 receptor (Kiss1R), which might be a key role factor of metastasis, and developing the stainer and SPECT probe for Kiss1R. Kisspeptin10 is a ligand of Kiss1R and consists of10 amino acid residues. Kisspeptin10 was derivatized as the stainer and SPECT probe. Kisspeptin10 stainer was visualized the localization of Kiss1R in pathological tissue sections of lung adenocarcinoma, lung squamous cell carcinoma, small cell lung carcinoma and medullary thyroid carcinoma. Next, Kisspeptin10 was labelled with 67Ga. 67Ga-labeled Kisspeptin10 was injected to the mouse bearing medullary thyroid carcinoma cells to take the SPECT/CT. As the result, Kisspeptin10 was highly accumulated in carcinoma. We concluded that the stainer and SPECT probe for Kiss1R were developed successfully.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (28 results)

All 2016 2015 2014

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Notch1 controls cell chemo-resistance in small cell lung carcinoma cells.2016

    • Author(s)
      Hassan WA, Yoshida R, Kudoh S, Kameyama H, Hasegawa K, Niimori-Kita K, Ito T
    • Journal Title

      Thoracic Cancer

      Volume: 7 Issue: 1 Pages: 123-128

    • DOI

      10.1111/1759-7714.12297

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Iodine-131 imaging using 284 keV photons with a small animal CZT-SPECT system dedicated to low-medium-energy photon detection.2016

    • Author(s)
      Kojima A, Gotoh K, Shimamoto M, Hasegawa K, Okada S.
    • Journal Title

      Ann Nucl Med.

      Volume: 30 Issue: 2 Pages: 169-175

    • DOI

      10.1007/s12149-015-1028-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hybrid Light Imaging Using Cerenkov Luminescence and Liquid Scintillation for Preclinical Optical Imaging In Vivo.2016

    • Author(s)
      Shimamoto M, Gotoh K, Hasegawa K, Kojima A.
    • Journal Title

      Mol Imaging Biol

      Volume: 18 Issue: 4 Pages: 500-509

    • DOI

      10.1007/s11307-016-0928-y

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Visualization and Quantitative Assessment of the Brain Distribution of Insulin through Nose-to-Brain Delivery Based on the Cell-Penetrating Peptide Noncovalent Strategy2016

    • Author(s)
      Kamei, Noriyasu; Shingaki, Tomotaka; Kanayama, Yousuke; Tanaka, Misa; Zochi, Riyo; Hasegawa, Koki; Watanabe, Yasuyoshi; Takeda-Morishita, Mariko.
    • Journal Title

      Molecular Pharmaceutics

      Volume: 13 (3) Issue: 3 Pages: 1004-1011

    • DOI

      10.1021/acs.molpharmaceut.5b00854

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of 64Cu and 68Ga for Molecular Imaging of Atherosclerosis using the Apolipoprotein A-I Mimetic Peptide FAMP2015

    • Author(s)
      Yahiro, Eiji; Kawachi, Emi; Miura, Shin-Ichiro; Kuwano, Takashi; Imaizumi, Satoshi; Iwata, Atsushi; Hasegawa, Koki; Yano, Tsuneo; Watanabe, Yasuyoshi; Uehara, Yoshinari; Saku, Keijiro
    • Journal Title

      Journal of Cardiovascular Diseases & Diagnosis

      Volume: 3 Issue: 03 Pages: 1-6

    • DOI

      10.4172/2329-9517.1000201

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insulinoma-Associated protein 1 is a crucial regulator of neuroendocrine differentiation in lung cancer2015

    • Author(s)
      Fujino, Kosuke; Motooka, Yamato; Wael, Hassan; Ali Abdalla, Mohamed; Sato, Yonosuke; Kudoh, Shinji; Hasegawa, Koki; Niimori-Kita, Kanako; Kobayashi, Hironori; Kubota, Ichiro; Wakimoto, Joeji; Suzuki, Makoto; Takaaki, Ito
    • Journal Title

      The American Journal of Pathology

      Volume: 185 Issue: 12 Pages: 3164-3177

    • DOI

      10.1016/j.ajpath.2015.08.018

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Notch1 controls cell invasion and metastasis in small cell lung carcinoma cell lines.2014

    • Author(s)
      Hassan Wael; Ryoji Yoshida; Shinji Kudoh; Kohki Hasegawa; Kanako Niimori-Kita; Takaaki Ito
    • Journal Title

      Lung cancer

      Volume: 86(3) Issue: 3 Pages: 304-310

    • DOI

      10.1016/j.lungcan.2014.10.007

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke2014

    • Author(s)
      Niimori-Kita K, Ogino K, Mikami S, Kudoh S, Koizumi D, Kudoh N, Nakamura F, Misumi M, Shimomura T, Hasegawa K, Usui F, Nagahara N, Ito T
    • Journal Title

      FEBS Open Bio

      Volume: 4 Issue: 1 Pages: 746-754

    • DOI

      10.1016/j.fob.2014.08.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Notch1 signaling controls cell proliferation, apoptosis and differentiation in lung carcinoma2014

    • Author(s)
      Hassan Wael; Ryoji Yoshida; Shinji Kudoh; Kohki Hasegawa; Kanako Niimori-Kita; Takaaki Ito
    • Journal Title

      Lung Cancer

      Volume: 85(2) Issue: 2 Pages: 131-140

    • DOI

      10.1016/j.lungcan.2014.05.001

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bromodeoxyuridine (BrdU)-label-retaining cells in mouse terminal bronchioles2014

    • Author(s)
      Hiroki Kameyama; Shinji Kudoh; Naoko Udaka; Motoko Kagayama; Wael Hassan; Kohki Hasegawa; Kanako Niimori-Kita; Takaaki Ito
    • Journal Title

      Histology and Histopathology

      Volume: 29(5) Pages: 659-668

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Visualization of cancer metastasis with radiolabeled ligands of Kiss1 receptors by SPECT2016

    • Author(s)
      Kumiko Gotoh, Koki Hasegawa, Seiji Okada
    • Organizer
      26th Federation of Asian Pharmaceutical Associations Congress
    • Place of Presentation
      Bangkok
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Kiss1受容体発現腫瘍スクリーニングと67Ga-DOTA-Kisspeptin10を用いたSPECTイメージング2016

    • Author(s)
      長谷川功紀, 前泊里佳, 後藤久美子, 古嶋昭博, 伊藤隆明
    • Organizer
      第56回日本核医学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2016-11-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] Detection of cholecystokinin receptor in formalin-fixed paraffin-embedded tissue sections using CCK8 derivative2016

    • Author(s)
      Koki Hasegawa, Rika Maedomari, Shinji Kudoh, Takaaki Ito
    • Organizer
      第53回ペプチド討論会
    • Place of Presentation
      京都
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] リガンド誘導体染色法を用いたコレシストキニン受容体の検出2016

    • Author(s)
      長谷川功紀, 前泊里佳, 伊藤隆明
    • Organizer
      第57回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] 肺癌におけるkisspeptin Receptor(GPR54)の発現2016

    • Author(s)
      前泊里佳, 長谷川功紀, 伊藤隆明
    • Organizer
      第57回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] 腎指向型アルブミン-BMP7ハイブリッド体の創製と腎保護効果の検討2015

    • Author(s)
      戸田翔太、渡邊博志、弥永直樹、濱崎慶輔、國安明彦、長谷川功紀、後藤久美子、異島優、小田切優樹、丸山徹
    • Organizer
      第32回日本薬学会九州支部大会
    • Place of Presentation
      宮崎
    • Year and Date
      2015-11-28
    • Related Report
      2015 Research-status Report
  • [Presentation] β線、γ線放出核種のハイブリッド光イメージングにおける最適化に関する基礎的検討2015

    • Author(s)
      嶋本雅子、後藤久美子、長谷川功紀、古嶋昭博
    • Organizer
      第55回日本核医学会学術総会 第35回日本核医学技術学会総会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-11-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 小動物用CZT-SPECT装置の性能評価のための3Dプリンタによるファントム作製2015

    • Author(s)
      後藤久美子、嶋本雅子、長谷川功紀、古嶋昭博
    • Organizer
      第55回日本核医学会学術総会 第35回日本核医学技術学会総会学術大会
    • Place of Presentation
      東京
    • Year and Date
      2015-11-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 肺癌におけるZMYM3 の発現について2015

    • Author(s)
      工藤仁孝、長谷川功紀、伊藤隆明
    • Organizer
      第56回平成27年度日本組織細胞化学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] INSM1 陽性肺神経内分泌細胞について2015

    • Author(s)
      本岡大和、長谷川功紀、伊藤隆明
    • Organizer
      第56回平成27年度日本組織細胞化学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] リガンド誘導体染色(LDS)法の開発とその応用2015

    • Author(s)
      長谷川功紀、伊藤隆明
    • Organizer
      第56回平成27年度日本組織細胞化学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Research-status Report
  • [Presentation] 機能プロテオミクスによる肺癌神経内分泌分化マーカーの探索2015

    • Author(s)
      工藤信次、新森加奈子、長谷川功紀、市村隆也、伊藤隆明
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 肺癌細胞におけるZinc-finger転写因子INSM1の意味2015

    • Author(s)
      藤野孝介、工藤信次、新森加奈子、長谷川功紀、伊藤隆明
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report
  • [Presentation] リガンド誘導体染色法を用いたFFPE切片上での分子イメージングプローブのスクリーニング法開発2015

    • Author(s)
      長谷川功紀、工藤信次、新森加奈子、伊藤隆明
    • Organizer
      第104回日本病理学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-04-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 臨床研究に向けた68Ga‐DOTA‐FAMP調製法の最適化2014

    • Author(s)
      長谷川功紀, 上原吉就, 川内絵未, 吉田毅, 矢野恒夫, 三浦伸一郎, 朔啓二郎
    • Organizer
      日本核医学会学術総会
    • Place of Presentation
      大阪府大阪市大阪国際会議場
    • Year and Date
      2014-11-06 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Kisspeptin誘導体を用いたGPR54(Kiss1R)染色法の開発2014

    • Author(s)
      長谷川功紀, 伊藤隆明
    • Organizer
      日本組織細胞化学会総会
    • Place of Presentation
      長野県松本市中央公民館
    • Year and Date
      2014-09-27 – 2014-09-28
    • Related Report
      2014 Research-status Report
  • [Presentation] 低分子リガンドを用いた受容体染色法の開発とカルチノイド腫瘍の染色2014

    • Author(s)
      長谷川功紀, 内藤嘉紀, 工藤信次, 新森加納子, 伊藤隆明
    • Organizer
      日本病理学会
    • Place of Presentation
      広島県広島市広島国際会議場
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report
  • [Book] 次世代ペプチド医薬創製2014

    • Author(s)
      長谷川功紀
    • Total Pages
      8
    • Publisher
      メディカルドゥ
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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