The significance of CD169+ macrophages in patients with malignant tumors

• Project/Area Number: 26460454
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Kumamoto University
• Principal Investigator: OHNISHI Koji 熊本大学, 大学院生命科学研究部(医), 助教 (40613378)
• Co-Investigator(Kenkyū-buntansha): 菰原 義弘 熊本大学, 大学院生命科学研究部(医), 准教授 (40449921)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: マクロファージ / リンパ節 / CD169 / 癌免疫 / リンパ節マクロファージ / がん免疫 / 腫瘍免疫 / 分子マーカー

Outline of Final Research Achievements

As the expectation for cancer immunotherapy increases, the importance of cancer immunity monitoring is increasing. We focused on the importance of lymph node macrophages in cancer immunity and performed the following analysis. CD 169 expression of lymph node macrophages was analyzed in patients with melanoma, uterine body cancer, pancreatic cancer, breast cancer. As a result, it was revealed that cancer immunity mediated by CD8 positive T cells is elevated in cases with high CD 169 expression, and survival rate after surgery is good. In culture experiments, it was revealed that CD169 highly expressed macrophages highly express CCL8 and CXCL10 having lymphocyte activating action.

Research Products

• [Journal Article] Contribution of Macrophage Polarization to Metabolic Diseases (2016)
  • Author(s): Yoshihiro Komohara, Yukio Fujiwara, Koji Ohnishi, Daisuke Shiraishi, Motohiro Takeya
  • Journal Title: Journal of Atherosclerosis and Thrombosis Volume: 23 Issue: 1 Pages: 10-17
  • DOI: 10.5551/jat.32359
  • NAID: 130005118655
  • ISSN: 1340-3478, 1880-3873
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Infiltration of tumor-associated macrophages is involved in CD44 expression in clear cell renal cell carcinoma. (2016)
  • Author(s): Ma C, Komohara Y, Ohnishi K, Shimoji T, Kuwahara N, Sakumura Y, Matsuishi K, Fujiwara Y, Motoshima T, Takahashi W, Yamada S, Kitada S, Fujimoto N, Nakayama T, Eto M, Takeya M.
  • Journal Title: Cancer Sci. Volume: Epub Issue: 5 Pages: 700-707
  • DOI: 10.1111/cas.12917
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic significance of CD169-positive lymph node sinus macrophages in patients with endometrial carcinoma. (2016)
  • Author(s): Ohnishi K, Yamaguchi M, Erdenebaatar C, Saito F, Tashiro H, Katabuchi H, Takeya M, Komohara Y.
  • Journal Title: Cancer Sci Volume: - Issue: 6 Pages: 846-852
  • DOI: 10.1111/cas.12929
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] TIM-3 expression in lymphoma cells predicts chemoresistance in patients with adult T-cell leukemia/lymphoma (ATLL). (2016)
  • Author(s): Horlad H, Ohnishi K, Ma C, Fujiwara Y, Niino D, Ohshima K, Jinushi M, Matsuoka M, Takeya M, Komohara Y.
  • Journal Title: Oncol Lett Volume: 12 Issue: 2 Pages: 1519-1524
  • DOI: 10.3892/ol.2016.4774
  • Peer Reviewed / Open Access
• [Journal Article] Calpain-6 confers atherogenicity to macrophages by dysregulating pre-mRNA splicing. (2016)
  • Author(s): Miyazaki T, Tonami K, Hata S, Aiuchi T, Ohnishi K, Lei XF, Kim-Kaneyama JR, Takeya M, Itabe H, Sorimachi H, Kurihara H, Miyazaki A
  • Journal Title: J Clin Invest Volume: 126 Issue: 9 Pages: 3417-3432
  • DOI: 10.1172/jci85880
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression of anaphylatoxin C5a receptor in gastric cancer: Implications with vascular invasion and patient outcome. (2016)
  • Author(s): Nitta, H., Shimose, T., Emi, Y., Imamura, T., Ohnishi, K., Kusumoto, T., Yamamoto. M., Fukuzawa, K., Takahashi, I., Higashi, H., Tsuji, A., Akagi, Y., Oki, E., Maehara, Y., & Baba, H.
  • Journal Title: Med. Oncol. Volume: 33 Issue: 11 Pages: 118-118
  • DOI: 10.1007/s12032-016-0834-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Clinical Significance of CD169-Positive Lymph Node Macrophage in Patients with Breast Cancer. (2016)
  • Author(s): Shiota T, Miyasato Y, Ohnishi K, Yamamoto-Ibusuki M, Yamamoto Y, Iwase H, Takeya M, Komohara Y.
  • Journal Title: PLoS One Volume: 11 Issue: 11 Pages: e0166680-e0166680
  • DOI: 10.1371/journal.pone.0166680
  • Peer Reviewed / Open Access
• [Journal Article] Guanylate-binding protein 5 is a marker of interferon-γ- induced classically activated macrophages. (2016)
  • Author(s): Fujiwara Y, Hizukuri Y, Yamashiro K, Makita N, Ohnishi K, Takeya M, Komohara Y, Hayashi Y.
  • Journal Title: Clin Transl Immunology Volume: 5 Issue: 11
  • DOI: 10.1038/cti.2016.59
  • Peer Reviewed / Open Access
• [Journal Article] Tumor-associated macrophages: Potential therapeutic targets for anti-cancer therapy. (2015)
  • Author(s): Yoshihiro Komohara, Yukio Fujiwara, Koji Ohnishi, Motohiro Takeya
  • Journal Title: Advanced drug delivery reviews Volume: 24 Pages: 180-185
  • DOI: 10.1016/j.addr.2015.11.009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The coordinated actions of TIM-3 on cancer and myeloid cells in the regulation of tumorigenicity and clinical prognosis in clear cell renal cell carcinomas (2015)
  • Author(s): Yoshihiro Komohara, Tomoko Morita, Dorcas A. Annan, Hasita Horlad, Koji Ohnishi, Sohsuke Yamada, Toshiyuki Nakayama, Shohei Kitada, Shinya Suzu, Ichiro Kinoshita, Hirotoshi Dosaka-Akita, Koichi Akashi, Motohiro Takeya, Masahisa Jinushi
  • Journal Title: Cancer immunology research Volume: 3 Issue: 9 Pages: 999-1007
  • DOI: 10.1158/2326-6066.cir-14-0156
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prognostic Significance of CD169+ Lymph Node Sinus Macrophages in Patients with Malignant Melanoma. (2015)
  • Author(s): Saito Y, Ohnishi K, Miyashita A, Nakahara S, Fujiwara Y, Horlad H, Motoshima T, Fukushima S, Jinnin M, Ihn H, Takeya M, Komohara Y.
  • Journal Title: Cancer Immunol Res. Volume: 3 Issue: 12 Pages: 1356-1363
  • DOI: 10.1158/2326-6066.cir-14-0180
  • Peer Reviewed / Open Access
• [Journal Article] A case of occult intrahepatic cholangiocarcinoma diagnosed by autopsy. (2015)
  • Author(s): Eri Oda, Daisuke Hashimoto, Yuko Shiomi, Koji Ohnishi, Hiromitsu Hayashi, Akira Chikamoto, Motohiro Takeya, Hideo Baba
  • Journal Title: Surgical case reports Volume: 1 Issue: 1 Pages: 101-101
  • DOI: 10.1186/s40792-015-0106-5
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of HIC-5 in the formation of microvilli-like structures and the monocyte-endotherial interaction that accelerates atherosclerosis (2015)
  • Author(s): Shigeko Arita-Okubo, et al
  • Journal Title: Cardiovasc Res Volume: 105 Issue: 3 Pages: 361-371
  • DOI: 10.1093/cvr/cvv003
  • Peer Reviewed / Open Access
• [Journal Article] Role of tumor-associated macrophages in hematological malignancies. (Review) (2015)
  • Author(s): Komohara Y, Niino D, Ohnishi K, Ohshima K, Takeya M
  • Journal Title: Pathol Int Volume: 65 Issue: 4 Pages: 170-176
  • DOI: 10.1111/pin.12259
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of CD204-Positive Tumor-Associated Macrophages in Adult T-Cell Leukemia/Lymphoma (2014)
  • Author(s): Yoichi Saito, Yoshihiro Komohara, Daisuke Niino, Hasita Horlad, Koji Ohnishi, Hiroto Takeya, Hiroya Kawaguchi, Hirokazu Shimizu, Koichi Ohshima, Motohiro Takeya
  • Journal Title: Journal of Clinical and Experimental Hematopathology Volume: 54 Issue: 1 Pages: 59-65
  • DOI: 10.3960/jslrt.54.59
  • NAID: 130004886477
  • ISSN: 1346-4280, 1880-9952
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 悪性腫瘍患者におけるCD169陽性リンパ節洞マクロファージの臨床的意義. (2016)
  • Author(s): 大西紘二、西東洋一、塩田拓也、ハスタ ホルラド、藤原章雄、竹屋元裕、菰原義弘.
  • Organizer: 第62回 日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2016-11-10
• [Presentation] がん患者の抗腫瘍免疫におけるCD169陽性リンパ節洞マクロファージの重要性. (2016)
  • Author(s): 大西紘二、菰原義弘、西東洋一、藤原章雄、竹屋元裕.
  • Organizer: 第56回 日本リンパ網内系学会総会
  • Place of Presentation: 熊本
  • Year and Date: 2016-09-01
• [Presentation] The Clinical relevance and prognostic significance of CD169+ lymph node macrophages in human malignant tumors. (2016)
  • Author(s): Koji Ohnishi, Yoichi Saito, Takuya Shiota, Hasita Horlad, Yukio Fujiwara, Motohiro Takeya, Yoshihiro Komohara.
  • Organizer: The 24th International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: Tokyo
  • Year and Date: 2016-06-04
  • Int'l Joint Research
• [Presentation] 悪性腫瘍におけるCD169陽性リンパ節マクロファージの臨床的意義 (2015)
  • Author(s): 大西紘二、西東洋一、菰原義弘、竹屋元裕
  • Organizer: 第104回 日本病理学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-04-30
• [Presentation] CD169-positive macrophages in regional lymph nodes involved in the tumor immunity and prognosis in patients with colorectal cancer, melanoma and endometrial cancer (2014)
  • Author(s): Koji Ohnishi, Yoshihiro Komohara, Yoichi Saito, Motohiro Takeya
  • Organizer: The 22nd International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: 神戸
  • Year and Date: 2014-06-02 – 2014-06-03
• [Presentation] Clinical significance of CD169-positive lymph node macrophages in human malignant tumors (2014)
  • Author(s): Koji Ohnishi, Yoichi Saito, Yoshihiro Komohara, Yuji Miyamoto, Satoshi Fukushima, Munekage Yamaguchi, Hideo Baba, Hironobu Ihn, Hidetaka Katabuchi, Motohiro Takeya
  • Organizer: 2014 ASCO Annual Meeting
  • Place of Presentation: Chicago
  • Year and Date: 2014-05-30 – 2014-06-01
• [Presentation] 大腸癌患者における予後予測因子としてのCD169陽性リンパ節マクロファージの重要性 (2014)
  • Author(s): 大西紘二、菰原義弘、西東洋一、竹屋元裕
  • Organizer: 第103回 日本病理学会総会
  • Place of Presentation: 広島
  • Year and Date: 2014-04-24 – 2014-04-26
• [Book] 別冊BIO Clinica 慢性炎症と疾患 5:(1) 慢性炎症とがん (2016)
  • Author(s): 大西紘二、菰原義弘、竹屋元裕.
  • Total Pages: 6
  • Publisher: 北隆館
• [Book] 別冊BIO Clinica（バイオクリニカ） (2016)
  • Author(s): 大西紘二, 菰原義弘, 竹屋元裕
  • Total Pages: 6
  • Publisher: 北隆館
• [Remarks] researchmap
  • URL: http://researchmap.jp/read0132383/