| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Occludin, a tight junction transmembrane protein, has 2 cysteine residues in its extracellular loop, which affect its stability and subcellular distribution. Under hypoxia, occludin degradation occurs in the cytoplasm, but occludin in the cytoplasmic membrane is stable. These results suggest that there is disulfide bond formation in or between occludin molecules. Occludin requires ITCH, a ubiquitin E3 ligase, for its degradation via poly-ubiquitination. Occludin interacts with other tight junction proteins, such as tricellulin and claudin-4, but there is no difference in its interactions with tight junction proteins in the presence or absence of cysteine mutations.
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