| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We estimated the telomere lengths (TLs) of mesothelial cells and neoplastic cells in pleural effusion and examined their correlation with asbestos exposure and expression of markers of mesothelial malignancy. Cell blocks of pleural effusion obtained from 35 cases of non-neoplastic disease (NN), 9 cases of malignant mesothelioma (MM) and 8 cases of lung adenocarcinoma (LA) were examined. 15 of 35 NN cases had pleural plaques (NNpp+) suggestive of asbestos exposure, and other 20 had no pleural plaques (NNpp-). TL was measured using tissue quantitative FISH method. NN had significantly longer TLs than MM (p<0.001) and LA (p<0.001). TLs in NNpp+ were slightly shorter than those of NNpp- (p=0.047). MM and LA showed almost the same TLs. NN with shorter TLs tended to show aberrant expression of EMA, CD146, GLUT1 and IMP3. These results suggest that telomere shortening and subsequent genetic instability play an important role in the development of MM.
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