Telomere length of mesothelial cells in pleural effusion, and its correlation with asbestos exposure and development of malignant mesothelioma
Project/Area Number |
26460457
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | International University of Health and Welfare |
Principal Investigator |
AIDA Shinsuke 国際医療福祉大学, 大学病院, 教授 (10531387)
|
Co-Investigator(Kenkyū-buntansha) |
相田 順子 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 専門副部長 (80425678)
|
Research Collaborator |
TSUURA Yukio
|
Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 中皮細胞 / 悪性中皮腫 / テロメア / 石綿 / 胸水 / アスベスト / 腺癌 / Q-FISH |
Outline of Final Research Achievements |
We estimated the telomere lengths (TLs) of mesothelial cells and neoplastic cells in pleural effusion and examined their correlation with asbestos exposure and expression of markers of mesothelial malignancy. Cell blocks of pleural effusion obtained from 35 cases of non-neoplastic disease (NN), 9 cases of malignant mesothelioma (MM) and 8 cases of lung adenocarcinoma (LA) were examined. 15 of 35 NN cases had pleural plaques (NNpp+) suggestive of asbestos exposure, and other 20 had no pleural plaques (NNpp-). TL was measured using tissue quantitative FISH method. NN had significantly longer TLs than MM (p<0.001) and LA (p<0.001). TLs in NNpp+ were slightly shorter than those of NNpp- (p=0.047). MM and LA showed almost the same TLs. NN with shorter TLs tended to show aberrant expression of EMA, CD146, GLUT1 and IMP3. These results suggest that telomere shortening and subsequent genetic instability play an important role in the development of MM.
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Report
(5 results)
Research Products
(6 results)