| Project/Area Number |
26460460
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
渋谷 和俊 東邦大学, 医学部, 教授 (20196447)
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| Research Collaborator |
YANAI Shun
SHIMODAIRA Kayoko
SHINOZAKI Minoru
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 特発性肺動脈性肺高血圧症 / Wnt/PCPパスウェイ / Daam-1 / Dvl-2 / 肺動脈中膜平滑筋細胞 / IPAH / WNT/PCPパスウェイ / 免疫組織染色 / Stachybotrys chartarum |
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| Outline of Final Research Achievements |
Evidence from our recent study suggests that idiopathic pulmonary arterial hypertension (IPAH) pathogenesis is related to upregulation of the Wnt/PCP pathway. We used microscopic observation and immunohistochemical techniques to identify expression patterns of cascading proteins - namely Wnt-11, Dvl-2, and Daam-1 - in pulmonary arteries.
We analyzed sections of formalin-fixed and paraffin-embedded autopsied lung tissues obtained 9 IPAH cases, 7 associated pulmonary arterial hypertension cases, and 16 age-matched controls without pulmonary arterial abnormalities, and marked expression of Dvl-2 and Daam-1 was confirmed in smooth muscle cells. In addition, Dvl-2 was depleted while Daam-1 expression was elevated in IPAH, in contrast with specimens from associated pulmonary arterial hypertension cases and controls. So it was suggested that high Daam-1 expression may upregulate the Wnt/PCP pathway and cause IPAH.
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