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Elucidation of SOX2 transcriptional regulation mechanism using genome editing technique

Research Project

Project/Area Number 26460465
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Experimental pathology
Research InstitutionShimane University

Principal Investigator

Iizasa HISASHI  島根大学, 医学部, 准教授 (80306662)

Co-Investigator(Kenkyū-buntansha) 金廣 優一  島根大学, 医学部, 助教 (60609197)
Co-Investigator(Renkei-kenkyūsha) KURAMITU Yasuhiro  山口大学, 医学部, 准教授 (50281811)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsゲノム編集 / 幹細胞 / SOX2
Outline of Final Research Achievements

Cas9, gRNA expression vectors, and homologous recombination vector were transfected into breast cancer cell line MCF-7 using genome editing. fluorescent genes Venus was inserted into the C-terminal side of the SOX2 gene. In the cell line of the homologous recombinant, although it localized in the nucleus and emitted green fluorescence, the fluorescence was weak, and it was difficult to analyze the localization of intracellular cells as live cells by fluorescence microscopy. In addition, there was variation in cell proliferation for each cell line compared to the parent strain, and off - target by gRNA was predicted. Similar results were found in genomic editing of other genes. Therefore, Cas9 protein and gRNA complex were formed in vitro and introduced. Currently, we analyze new homologous recombinants.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (42 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (5 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (25 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (4 results) Remarks (1 results)

  • [Int'l Joint Research] Mahidol University(Thailand)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 大連大学付属中山病院(中国)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] ウィスター研究所(米国)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] マヒドン大学(タイ)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] 国立科学研究センター(フランス)

    • Related Report
      2015 Research-status Report
  • [Journal Article] EBウイルス感染症(特集:古くて新しい日和見感染症)2017

    • Author(s)
      金廣優一、飯笹久、吉山裕規.
    • Journal Title

      臨床と微生物

      Volume: 44 Pages: 69-76

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] DNA/RNA編集研究の新たな眺望2016

    • Author(s)
      村松正道、飯笹久
    • Journal Title

      生化学

      Volume: 88 Issue: 5 Pages: 555-556

    • DOI

      10.14952/SEIKAGAKU.2016.880555

    • NAID

      120005905791

    • ISSN
      0037-1017
    • Year and Date
      2016-10-25
    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] Pathophysiological role of A-to-I RNA editing enzyme ADAR1 in human diseases2016

    • Author(s)
      飯笹久
    • Journal Title

      生化学

      Volume: 88 Issue: 5 Pages: 593-599

    • DOI

      10.14952/SEIKAGAKU.2016.880593

    • ISSN
      0037-1017
    • Year and Date
      2016-10-25
    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] Sox2 functionally interacts with βAPP, the βAPP intracellular domain and ADAM10 at a transcriptional level in human cells.2016

    • Author(s)
      Sarlak G, Htoo HH, Hermanndez JF, Iizasa H, Checker F, Konietzko U, Song W, Vincent B.
    • Journal Title

      Neuroscience

      Volume: 312 Pages: 153-164

    • DOI

      10.1016/j.neuroscience.2015.11.022

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dysbiotic infection in the stomach.2015

    • Author(s)
      Iizasa H, Ishihara S, Richardo T, Kanehiro Y, Yoshiyama H.
    • Journal Title

      Word J Gastroenterol

      Volume: 21 Issue: 40 Pages: 11450-11457

    • DOI

      10.3748/wjg.v21.i40.11450

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clustered microRNAs of the Epstein-Barr virus cooperatively downregulate an epithelial cell-specific metastasis suppressor.2015

    • Author(s)
      Kanda T, Miyata M, Kano M, Kondo S, Yoshizaki T, Iizasa H.
    • Journal Title

      Journal of Virology

      Volume: 89 Issue: 5 Pages: 2684-2697

    • DOI

      10.1128/jvi.03189-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Epstein-barr virus in gastric carcinoma.2014

    • Author(s)
      Nishikawa J, Yoshiyama H, Iizasa H, Kanehiro Y, Nakamura M, Nishimura J, Saito M, Okamoto T, Sakai K, Suehiro Y, Yamasaki T, Oga A, Yanai H, Sakaida I.
    • Journal Title

      Cancers

      Volume: 6 Issue: 4 Pages: 2259-2274

    • DOI

      10.3390/cancers6042259

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 関節炎モデルにおけるmicroRNA-381による治療効果.2016

    • Author(s)
      田中陽子、高田修治、飯笹久、Artemis Hatzigeorgoiu、宮澤慎一、古松穀之、西田圭一郎、浅原弘嗣.
    • Organizer
      第39回日本分子生物学会年会.
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] EBウイルスがコードするBART microRNAプロモーターの制御機構.2016

    • Author(s)
      Hyoji Kim、飯笹久、金廣優一、Timmy Richardo、吉山裕規.
    • Organizer
      第64回日本ウイルス学会学術集会.
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Epstein-Barrウイルス由来エキソソームの機能解析.2016

    • Author(s)
      南保明日香、片野晴隆、飯笹久、吉山裕規、Bill Sugden、大場雄介.
    • Organizer
      第64回日本ウイルス学会学術集会.
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] EBV感染は胃上皮細胞のミトコンドリアDNAにAPOBEC3依存的な変異を導入する.2016

    • Author(s)
      金廣優一、Timmy Richardo 、Hyoji Kim、飯笹久、吉山裕規.
    • Organizer
      第64回日本ウイルス学会学術集会.
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] EBV感染は胃上皮細胞においてAPOBEC3依存的なミトコンドリアDNA変異を誘導する.2016

    • Author(s)
      飯笹久、金廣優一、Timmy Richardo、Hyoji Kim、村松正道、吉山裕規.
    • Organizer
      第75回日本癌学会学術集会.
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] microRNA-381 ameliorates arthritis by controlling functions of fibroblast-like synoviocytes, which are affected by A to I RNA editing.2016

    • Author(s)
      Tanaka Y, Takada S, Iizasa H, Hatzigeorgiou A, Miyazawa SI, Furumatsu T, Nishida K, Asahara H.
    • Organizer
      European Society of Cardiology Congress 2016.
    • Place of Presentation
      London LONDON Convention Center, London, UK
    • Year and Date
      2016-08-27
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EBV infection induces APOBEC3-dependent mitochondrial DNA mutation in epithelial cells.2016

    • Author(s)
      Kanehiro Y, Richardo T, Kim H, Iizasa H, Yoshiyama H.
    • Organizer
      17th International Symposium on EBV.
    • Place of Presentation
      University of Zurich (University Tower), Zurich, Switzerland
    • Year and Date
      2016-08-08
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EBウイルス由来BART microRNAプロモーター制御機構の解析.2016

    • Author(s)
      Hyoji Kim、飯笹久、金廣優一、Timmy Richardo、吉山裕規.
    • Organizer
      第31回中国四国ウイルス研究会.
    • Place of Presentation
      鳥取大学農学部大講義室(鳥取)
    • Year and Date
      2016-07-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] EBVの胃上皮細胞への感染は、APOBEC3の発現誘導を介して、ミトコンドリアDNAに変異を導入する.2016

    • Author(s)
      145. Timmy Richardo 、金廣優一、Hyoji Kim、飯笹久、吉山裕規.
    • Organizer
      第31回中国四国ウイルス研究会.
    • Place of Presentation
      鳥取大学農学部大講義室(鳥取)
    • Year and Date
      2016-07-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Bacterial coinfection augments Epstein-Barr virus infection of epithelial cells.2016

    • Author(s)
      Yoshiyama H, Iizasa H, Richardo T, Kanehiro Y, Kim H.
    • Organizer
      “Nasopharyngeal carcinoma” Gordon Research Conference.
    • Place of Presentation
      The Hong Kong University of Science and Techonology, Hong Kong, China
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Epstein-Barr virus-encoded microRNA miR-BART6 regulates viral latency and induces epithelial-mesenchymal transition.2016

    • Author(s)
      Iizasa H, KimH, Yoshiyama H.
    • Organizer
      “Nasopharyngeal carcinoma” Gordon Research Conference.
    • Place of Presentation
      The Hong Kong University of Science and Techonology, Hong Kong, China
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] EBVの胃上皮細胞への感染は、APOBEC3の発現誘導を介して、ミトコンドリアDNAに変異を導入する.2016

    • Author(s)
      金廣優一、Timmy Richardo 、Hyoji Kim、飯笹久、吉山裕規.
    • Organizer
      第30回ヘルペスウイルス研究会
    • Place of Presentation
      セミナーハウス クロス・ウェーブ府中(東京)
    • Year and Date
      2016-06-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] ピロリ菌感染はEBウイルスの胃上皮細胞における増殖を活性化する.2016

    • Author(s)
      飯笹久、金廣優一、溝手朝子、吉山裕規.
    • Organizer
      第89回日本細菌学会総会.
    • Place of Presentation
      大阪国際交流センター(大阪)
    • Year and Date
      2016-03-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Epstein-Barrウイルス由来BART microRNA転写制御領域の解析.2016

    • Author(s)
      飯笹久、Hyoji Kim、Timmy Richardo、金廣優一、吉山裕規.
    • Organizer
      第13回EBウイルス研究会.
    • Place of Presentation
      東京医科歯科大学(東京)
    • Related Report
      2016 Annual Research Report
  • [Presentation] RNA編集酵素ADAR2によるmiRNA産生制御と癌.2015

    • Author(s)
      飯笹久.
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(神戸)
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Evaluation of genomic instability of cells infected with Epstein-Barr virus.2015

    • Author(s)
      Hyoji Kim、飯笹久、金廣優一、Timmy Richardo、吉山裕規.
    • Organizer
      第63回日本ウイルス学会学術集会.
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] 低酸素刺激によって増強するヒト悪性胸膜中皮腫細胞の運動・浸潤能におけるHIF1およびMUC1の役割.2015

    • Author(s)
      項慧慧、ゴウダルジ・ホウマヌ、飯笹久、坂田健一郎、樋田泰浩、柳原五吉、久保貴紀、中川宏治、小林正伸、入村達郎、浜田淳一.
    • Organizer
      . 第74回日本癌学会学術集会.
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] RNA編集酵素ADAR2は、ヒト悪性中皮腫においてoncomiRのプロセッシングと悪性化を制御する。2015

    • Author(s)
      飯笹久、坂田健一郎、前田浩次郎、柳原五吉、久保貴紀、吉山裕規、程久美子、浜田淳一. RNA編集酵素ADAR2は、ヒト悪性中皮腫においてoncomiRのプロセッシングと悪性化を制御する。
    • Organizer
      第74回日本癌学会学術集会.
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Research-status Report
  • [Presentation] RNA編集酵素ADAR2は、oncomiRプロセッシングを制御する。2015

    • Author(s)
      飯笹久、坂田健一郎、Josephine Galipon、石黒宗、前田浩次郎、柳原五吉、久保貴紀、吉山裕規、程久美子、浜田淳一.
    • Organizer
      第17回日本RNA学会年会.
    • Place of Presentation
      ホテルライフォード札幌(札幌)
    • Year and Date
      2015-07-15
    • Related Report
      2015 Research-status Report
  • [Presentation] A-to-I RNA editing enzyme ADAR2 regulates malignancy in human pleural mesothelioma.2015

    • Author(s)
      Iizasa H, Kenichiro Sakata, Josephine Galipon, Kojiro Maeda, Soh Ishiguro, Nozomi Sakurai, Hironori Yoshiyama, Kazuyoshi Yanagihara, Takanori Kubo, Yoshimasa Kitagawa, Hideyuki Kawauchi, Ui-Tei Kumiko, Jun-ichi Hmada.
    • Organizer
      “RNA editing” Gordon Research Conference.
    • Place of Presentation
      Renaissance Tuscany II Ciocco Resort in Lucca, Barga, Italy
    • Year and Date
      2015-03-08 – 2015-03-12
    • Related Report
      2014 Research-status Report
  • [Presentation] RNA編集酵素ADAR2は悪性胸膜中皮腫細胞の増殖・運動・浸潤性を制御する.2014

    • Author(s)
      坂田健一郎、前田浩次郎、櫻井希、梁珊珊、中澤誠多朗、吉山裕規、柳原五吉、久保貴紀、北川善政、川内秀之、浜田淳一、飯笹久.
    • Organizer
      第73回日本癌学会学術集会.
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] パラペックル構成因子NonOは、SOX2プロモーター制御を介してがん幹細胞様表現型を抑制する.2014

    • Author(s)
      飯笹久、梁珊珊、高橋秀尚、廣瀬哲郎、藏滿保宏、中澤誠多朗、中村和行、畠山鎮次、川内秀之、吉山裕規、 浜田淳一
    • Organizer
      第73回日本癌学会学術集会.
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] RNA編集酵素ADAR2の発現抑制は悪性中皮腫細胞の増殖・運動・浸潤性を低下させる。2014

    • Author(s)
      坂田健一郎、前田浩次郎、櫻井希、梁珊珊、中澤誠多朗、吉山裕規、柳原五吉、久保貴紀、川内秀之、北川善政、浜田淳一、飯笹久.
    • Organizer
      第16回RNAミーティング.
    • Place of Presentation
      ウインクアイチ(名古屋)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] RNA-DNA結合タンパク質NonO は、SOX2プロモーター制御を介して乳癌幹細胞様表現型をコントロールする.2014

    • Author(s)
      飯笹久、梁珊珊、高橋秀尚、藏滿保宏、廣瀬哲郎、中澤誠多朗、畠山鎮次、川内秀之、吉山裕規、浜田淳一.
    • Organizer
      第16回RNAミーティング.
    • Place of Presentation
      ウインクアイチ(名古屋)
    • Year and Date
      2014-07-23 – 2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子欠損による上皮細胞の感染性喪失を指標とした EB ウイルス感染機構の解明.2014

    • Author(s)
      飯笹久、金廣優一、千葉朋希、川内秀之、浅原弘嗣、吉山裕規.
    • Organizer
      第11回EBウイルス研究会.
    • Place of Presentation
      国立感染症研究所(東京)
    • Year and Date
      2014-06-27
    • Related Report
      2014 Research-status Report
  • [Book] EBウイルス関連胃癌における最近の話題(EBウイルス関連胃癌).2016

    • Author(s)
      飯笹久、Hyoji Kim、吉山裕規.
    • Total Pages
      77
    • Publisher
      診断と治療社
    • Related Report
      2016 Annual Research Report
  • [Book] EB ウイルスの胃上皮細胞への感染と不死化(EBウイルス関連胃癌).2016

    • Author(s)
      金廣優一、Timmy Richardo、飯笹久、吉山裕規.
    • Total Pages
      77
    • Publisher
      診断と治療社
    • Related Report
      2016 Annual Research Report
  • [Book] 組換えEBウイルス作製法(EBウイルス改訂第3版)2015

    • Author(s)
      飯笹久、吉山裕規.
    • Total Pages
      209
    • Publisher
      診断と治療社
    • Related Report
      2015 Research-status Report
  • [Book] MicroRNAs and oncogenic human viruses (MicroRNAs: Key Regulators of Oncogenesis).2014

    • Author(s)
      Thirion M, Kanda T, Murakami Y, Ochiya T, Iizasa H.
    • Total Pages
      428
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report
  • [Remarks] 島根大学医学部微生物免疫学講座(微生物)ホームページ

    • URL

      http://yoshiyama-lab.org/

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-07  

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