Elucidation of SOX2 transcriptional regulation mechanism using genome editing technique
| Project/Area Number |
26460465
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Shimane University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金廣 優一 島根大学, 医学部, 助教 (60609197)
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| Co-Investigator(Renkei-kenkyūsha) |
KURAMITU Yasuhiro 山口大学, 医学部, 准教授 (50281811)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ゲノム編集 / 幹細胞 / SOX2 |
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| Outline of Final Research Achievements |
Cas9, gRNA expression vectors, and homologous recombination vector were transfected into breast cancer cell line MCF-7 using genome editing. fluorescent genes Venus was inserted into the C-terminal side of the SOX2 gene. In the cell line of the homologous recombinant, although it localized in the nucleus and emitted green fluorescence, the fluorescence was weak, and it was difficult to analyze the localization of intracellular cells as live cells by fluorescence microscopy. In addition, there was variation in cell proliferation for each cell line compared to the parent strain, and off - target by gRNA was predicted. Similar results were found in genomic editing of other genes. Therefore, Cas9 protein and gRNA complex were formed in vitro and introduced. Currently, we analyze new homologous recombinants.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Epstein-barr virus in gastric carcinoma.2014
Author(s)
Nishikawa J, Yoshiyama H, Iizasa H, Kanehiro Y, Nakamura M, Nishimura J, Saito M, Okamoto T, Sakai K, Suehiro Y, Yamasaki T, Oga A, Yanai H, Sakaida I.
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Journal Title
Cancers
Volume: 6
Issue: 4
Pages: 2259-2274
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] microRNA-381 ameliorates arthritis by controlling functions of fibroblast-like synoviocytes, which are affected by A to I RNA editing.2016
Author(s)
Tanaka Y, Takada S, Iizasa H, Hatzigeorgiou A, Miyazawa SI, Furumatsu T, Nishida K, Asahara H.
Organizer
European Society of Cardiology Congress 2016.
Place of Presentation
London LONDON Convention Center, London, UK
Year and Date
2016-08-27
Related Report
Int'l Joint Research
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[Presentation] A-to-I RNA editing enzyme ADAR2 regulates malignancy in human pleural mesothelioma.2015
Author(s)
Iizasa H, Kenichiro Sakata, Josephine Galipon, Kojiro Maeda, Soh Ishiguro, Nozomi Sakurai, Hironori Yoshiyama, Kazuyoshi Yanagihara, Takanori Kubo, Yoshimasa Kitagawa, Hideyuki Kawauchi, Ui-Tei Kumiko, Jun-ichi Hmada.
Organizer
“RNA editing” Gordon Research Conference.
Place of Presentation
Renaissance Tuscany II Ciocco Resort in Lucca, Barga, Italy
Year and Date
2015-03-08 – 2015-03-12
Related Report
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