| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Reprogramming mediated control of pancreatic cancer cell lines were attempted in this study. Four Yamanaka factors were introduced into pancreatic cancer cell lines PK-8 and PK-9 as well as their derived gemcitabine (GEM herein after) resistant cell lines RPK-8 and RPK-9. Lentivirus vector was first used but results were not successful. We next tried TET-vector but results were not successful. However, we observed some unexpected interesting findings. GEM resistance in RPK-9 is caused by homozygous deletion of DCK that is essential for activation of GEM. Hence, RPK-9 can survive under very high concentration of GEM. On the other hand, RPK-8 can survive under low concentration of GEM but cannot survive under very high concentration. Furthermore, upregulation of genes associated with stemness and EMT were evident in RPK-8. Cell motility was also upregulated. These results may suggest that stemness associated with EMT is playing an important role in acquisition of GEM in RPK-8.
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