| Project/Area Number |
26460469
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUGIMURA haruhiko 浜松医科大学, 医学部, 教授 (00196742)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | DNA付加体 / アダクトーム / アミダイト / がん / 塩基除去修復酵素 / 有機合成 / 脂質酸化物 / 合成 / 塩基除去修 復酵素 |
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| Outline of Final Research Achievements |
Lipid-peroxidated products (OHE and ONE) were synthesized from the corresponding 2-substituted furans, which are commercially available. Thus obtained OHE (or ONE) were next reacted with 2’-deoxycytosine (or 2’-deoxyguanosine) performing the conjugate addition-cyclization to afford DNA adducts; namely, OHE-dC, OHE-dG, ONE-dC, and ONE-dG, respectively without HPLC separation. Furthermore, transformation to their amidites form these DNA adducts was effectively conducted. In addition, other kind of DNA adducts were synthesized.
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