Synthetic studies of DNA adducts and its amidites for DNA adductome analysis.
Project/Area Number |
26460469
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Experimental pathology
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
SUGIMURA haruhiko 浜松医科大学, 医学部, 教授 (00196742)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | DNA付加体 / アダクトーム / アミダイト / がん / 塩基除去修復酵素 / 有機合成 / 脂質酸化物 / 合成 / 塩基除去修 復酵素 |
Outline of Final Research Achievements |
Lipid-peroxidated products (OHE and ONE) were synthesized from the corresponding 2-substituted furans, which are commercially available. Thus obtained OHE (or ONE) were next reacted with 2’-deoxycytosine (or 2’-deoxyguanosine) performing the conjugate addition-cyclization to afford DNA adducts; namely, OHE-dC, OHE-dG, ONE-dC, and ONE-dG, respectively without HPLC separation. Furthermore, transformation to their amidites form these DNA adducts was effectively conducted. In addition, other kind of DNA adducts were synthesized.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Robust quantitative assessments of cytosine modifications and changes in the expressions of related enzymes in gastric cancer.2014
Author(s)
Du C, Kurabe N, Matsushima Y, Suzuki M, Kahyo T, Ohnishi I, Tanioka F, Tajima S, Goto M, Yamada H, Tao H, Shinmura K, Konno H, Sugimura H.
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Journal Title
Gastric Cancer.
Volume: 14
Issue: 3
Pages: 409-409
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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