Regularatory role of bFGF and microRNA for cardiac remodeling

• Project/Area Number: 26460480
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Toho University
• Principal Investigator: AKASAKA Yoshikiyo 東邦大学, 医学部, 教授 (60202511)
• Co-Investigator(Kenkyū-buntansha): 木下 利雄 東邦大学, 医学部, 助教 (00723719) ; 石川 由起雄 東邦大学, 医学部, 客員教授 (30276894) ; 鈴木 健也 東邦大学, 医学部, 助教 (20516231)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: mircoRNA / bFGF / 心筋間質 / 線維芽細胞 / リモデリング / microRNA / MicroRNA / 心筋リモデリング / 心筋間質細胞 / 線維化 / 高血圧 / 実験動物 / 間質線維化

Outline of Final Research Achievements

This study shows antifibrotic effects of bFGF injection in hypertensive DS rat hearts carried out using cardiac fibroblast lines harvested from the heart during the LV dilation phase. Specifically, we demonstrated that (1) bFGF inhibited interstitial fibrosis in hypertensive rat heart in vivo; (2) bFGF up-regulated TIMP-1 and down- regulated MMP-9 expression in cardiac fibroblasts (CFs) from hypertensive rat hearts; (3) bFGF also inhibited the expression of collagen I and III in the CFs; (4) real-time PCR array microRNA assays in bFGF treated CFs identified several microRNAs that were up-regulated in the CFs and specifically expressed in interstitial fibrosis of hypertensive DS rat hearts.

Research Products

• [Journal Article] 慢性心不全の病理：拡張不全型心不全における心筋組織再改築の分子制御 (2017)
  • Author(s): 赤坂喜清、藤澤千恵、浜之上誠、鈴木健也、木下利雄
  • Journal Title: 心電図 Volume: 37
  • Open Access
• [Journal Article] Antifibrotic response of cardiac fibroblasts in hypertensive hearts through enhanced TIMP-1 expression by basic fibroblast growth factor (2014)
  • Author(s): Kinoshita T, Ishikawa Y, Arita M, Akishima-Fukasawa Y, Fujita K, Inomata N, Suzuki T, Namiki A, Mikami T, Ikeda T, Yamazaki J, Ishii T, Akasaka Y.
  • Journal Title: Cardiovascular Pathology Volume: 23 Issue: 2 Pages: 92-100
  • DOI: 10.1016/j.carpath.2013.11.001
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 慢性心不全の病理：心筋組織再改築と間質繊維化による拡張性心不全の分子機序 (2016)
  • Author(s): 赤坂喜清
  • Organizer: 第43回比較心電図研究会
  • Place of Presentation: 東京大学弥生キャンパス(東京都文京区)
  • Year and Date: 2016-09-03
• [Presentation] bFGFによる心筋組織修復の制御と機能分子の探索 (2015)
  • Author(s): 木下俊雄
  • Organizer: 第45回日本創傷治癒学会
  • Place of Presentation: JPタワーホール&カンファレンス(東京都千代田区)
  • Year and Date: 2015-12-01
• [Presentation] Basic fibroblast growth factor inhibits fibrotic response of cardiac fibroblasts in hypertensive hearts through enhanced TIMP-1 expression (2015)
  • Author(s): Akasaka Y, Kinoshita T, Nakamichi M, Akishima - Fukasawa Y, Suzuki T, Ohnishi K, Ikeda T, Mikami T
  • Organizer: 2015 WHS Annual Meeting
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2015-05-01
  • Int'l Joint Research
• [Remarks] 平成27年度 私立大学戦略的研究基盤形成支援事業報告会
  • URL: http://www.med.toho-u.ac.jp/lab/sentan_lab_index/srfpu/20160325.html