Search for therapeutic targets for small cell lung cancer using a new orthotopic transplantation model.

• Project/Area Number: 26460481
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Microbial Chemistry Research Foundation
• Principal Investigator: SAKAMOTO Shuichi 公益財団法人微生物化学研究会, 微生物化学研究所 沼津支所, 主任研究員 (60346070)
• Co-Investigator(Renkei-kenkyūsha): INOUE Hiroyuki 公益財団法人微生物化学研究会, 微生物化学研究所動物施設, 研究員 (50601548)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 小細胞肺癌 / 癌転移 / 自然転移モデル / 治療標的 / 足場非依存性

Outline of Final Research Achievements

Small cell lung cancer (SCLC) accounts for nearly 20% of lung cancer cases and one of the most aggressive cancers with highly metastatic ability. In this study, we assessed anti-tumor activities of compounds and an antibody and searched the metastatic genes using our originally developed orthotopic metastasis model of SCLC. Our results are listed below.(1) Our model has the same aspects of SCLC patients and is useful for assessment of anti-tumor activities of drug seeds.(2) We identified a membranous protein as a good candidate for anti-tumor antibody-drug target. (3) We established a protocol for identification of genes differentially expressed between orthotopic tumors and metastatic tumors.

Research Products

• [Journal Article] New metastatic model of human small-cell lung cancer by orthotopic transplantation in mice (2015)
  • Author(s): Shuichi Sakamoto, Hiroyuki Inoue, Shunichi Ohba, Yasuko Kohda, Ihomi Usami, Tohru Masuda, Manabu Kawada and Akio Nomoto
  • Journal Title: Cancer Science Volume: 106 Issue: 4 Pages: 367-374
  • DOI: 10.1111/cas.12624
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 小細胞肺癌の自然転移モデルの開発とその応用 (2016)
  • Author(s): 坂本修一、川田学
  • Organizer: 第２５回日本がん転移学会学術集会
  • Place of Presentation: 米子コンベンションセンター（鳥取県米子市）
  • Invited
• [Presentation] 小細胞肺癌の同所移植モデルの移植巣及び転移巣形成における膜タンパク質CXADRの役割の解析 (2015)
  • Author(s): 坂本修一、梶川益紀、増田徹、川田学
  • Organizer: 第７４回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-09
• [Presentation] 小細胞肺癌の自然転移モデルにおける膜タンパク質CXADRの意義の検討 (2015)
  • Author(s): 坂本修一、川田学
  • Organizer: 第２４回日本がん転移学会学術集会・総会
  • Place of Presentation: シティプラザ大阪（大阪市）
  • Year and Date: 2015-07-23
• [Presentation] Development of a novel orthotopic transplantation model of human small cell lung cancer metastasis (2014)
  • Author(s): Shuichi Sakamoto, Manabu Kawada, Akio Nomoto
  • Organizer: 第７３回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2014-09-26
• [Presentation] ヒト小細胞肺癌由来の高転移性細胞に対する新規抗CXADR抗体の制がん活性の検討 (2014)
  • Author(s): 坂本 修一、川田 学
  • Organizer: 第２３回日本がん転移学会学術総会
  • Place of Presentation: 金沢ニューグランドホテル（金沢市）
  • Year and Date: 2014-07-10
• [Remarks] 公益財団法人微生物化学研究会 微生物化学研究所
  • URL: http://www.bikaken.or.jp