| Project/Area Number |
26460486
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
NIIMI Manabu 山梨大学, 総合研究部, 助教 (80644898)
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| Co-Investigator(Kenkyū-buntansha) |
西島 和俊 秋田大学, バイオサイエンス教育・研究サポートセンター, 准教授 (70435874)
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| Co-Investigator(Renkei-kenkyūsha) |
FAN Jianglin 山梨大学, 総合研究部, 教授 (60272192)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 動脈硬化 / 腹部大動脈瘤 / ウサギ / トランスジェニック / MMP-1 |
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| Outline of Final Research Achievements |
Increased expression of matrix metalloprotease-1 (MMP-1) has been implicated in atherosclerosis and aneurysms; however, it is not fully understood whether macrophage-derived MMP-1 affects atherosclerosis and aneurysm. To investigate this hypothesis, we generated transgenic (Tg) rabbits expressing human MMP-1 in macrophages under the control of human scavenger receptor promoter. After feeding a cholesterol rich diet, MMP-1 Tg rabbits had similar lesion size of aortic atherosclerosis compared with non-Tg rabbits; however, Tg rabbits showed a remarkable destruction of the medial elastic lamina in the aortic lesions. Using an elastase-induced aneurysm model, we found that Tg rabbits showed grater aortic dilation than non-Tg rabbits. These results indicate that macrophage-derived MMP-1 plays an important role in pathogenesis of vascular disease.
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