Analysis of osteoclastogenesis by intra- and extra-medullary regulation to prevent osteoporosis

• Project/Area Number: 26460488
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tottori University
• Principal Investigator: Hayashi Shin-Ichi 鳥取大学, 医学部, 教授 (50208617)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 炎症 / 破骨細胞 / 骨髄細胞 / 腹腔細胞 / 自然リンパ球 / 骨吸収 / 免疫学

Outline of Final Research Achievements

To prevent post-menopausal osteoporosis, persistent and reliable therapy is required. For this purpose, I am studying the regulation mechanisms of development of osteoclasts which resorb bone. I detected the osteoclastogenesis in both male and female mice is controlled by intra- and extra-medullary regulation. Cells for latter regulation are present in peritoneal cavities, and once lost, are not to be recovered. The flow cytometric analysis of bone marrow T cells did not show any significant difference with or without hypotonic treatment for removing peritoneal cells; however, the response to peritoneal cells is dependent on bone marrow T cells. The phenotype in these treated mice resembles the ovariectomized one carrying osteoporosis, suggesting the presence of common pathway in both conditions. The decision of the regulatory cells in the peritoneal cavity will result in the development of novel therapy for osteoporosis.

Research Products

• [Journal Article] Correlation between cell aggregation and antibody production in IgE-producing plasma cells. (2017)
  • Author(s): Hikosaka, M., Murata, A., Yoshino, M., and Hayashi, S.I.
  • Journal Title: Biochemistry and Biophysics Reports Volume: in press Pages: 224-231
  • DOI: 10.1016/j.bbrep.2017.04.007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Notch-mediated cell adhesion (2016)
  • Author(s): Akihiko Murata, Shin-Ichi Hayashi
  • Journal Title: Biology(Basel) Volume: 5 Issue: 1 Pages: 5-5
  • DOI: 10.3390/biology5010005
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] An evolutionary-conserved function of mammalian notch family members as cell adhesion molecules. (2014)
  • Author(s): Akihiko Murata, Miya Yoshino, Mari Hikosaka, Kazuki Okuyama, Lan Zhou, Seiji Sakano, Hideo Yagita, Shin-Ichi Hayashi
  • Journal Title: PLoS One Volume: 9 Issue: 9 Pages: e108535-e108535
  • DOI: 10.1371/journal.pone.0108535
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] C57BL/6 mice lack the local skin memory formation by contact dermatitis. (2016)
  • Author(s): Murata, A., Yoshino, M., Hikosaka, M., Hayashi, SI.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-07
• [Presentation] Enhancement of antibody production in IgE producing cells requires cell aggregation via Fc gamma-receptors. (2016)
  • Author(s): Hikosaka, M., Murata, A., Yoshino, M., Hayashi, SI.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-07
• [Presentation] Measurement of altered transport of skin self-antigens in the active state. (2016)
  • Author(s): Yoshino, M., Murata, A., Hikosaka, M., Suekane, K., Suzuki, A., Honda, N., Hayashi, SI.
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] Intensity of local skin memory response varying with the type of locally persisting T cell subsets. (2016)
  • Author(s): Murata, A., Yoshino, M., Hikosaka, M., Hayashi, SI.
  • Organizer: International Congress of Immunology 2016
  • Place of Presentation: Melbourne Convention and Exhibition Centre, Melbourne, Australia
  • Year and Date: 2016-08-25
  • Int'l Joint Research
• [Presentation] Analysis of the steady-state transport of skin self-antigens in Pynod-Ccr7-double knockout mice. (2016)
  • Author(s): Yoshino, M., Murata, A., Hikosaka, M., Suda, T., Hayashi, SI.
  • Organizer: The 24th International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: Sola City Conference Center (東京都千代田区)
  • Year and Date: 2016-06-04
  • Int'l Joint Research
• [Presentation] Antigen-mediated cell aggregation in IgE producing cells. (2015)
  • Author(s): Hikosaka, M., Murata, A., Shimoda, Y., Yoshino, M., Hayashi, SI.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（札幌）
  • Year and Date: 2015-11-20
• [Presentation] Analysis of the transport of skin self antigens in Pynod-Ccr7-double knockout mice. (2015)
  • Author(s): Yoshino, M., Murata, A., Hikosaka, M., Suda, T., Hayashi SI.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（札幌）
  • Year and Date: 2015-11-20
• [Presentation] Mast cells are dispensable for the local skin memory response. (2015)
  • Author(s): Akihiko, M., Yoshino, M., Hikosaka, M., Hayashi, SI.
  • Organizer: 第44回日本免疫学会学術集会
  • Place of Presentation: 札幌コンベンションセンター（札幌）
  • Year and Date: 2015-11-18
• [Presentation] Pynod is not essentially required for the transport of skin self-antigens to regional lymph nodes (2015)
  • Author(s): Yoshino, M., Murata, A., Hikosaka, M., Suda, T., Hayashi, SI.
  • Organizer: 国際ランゲルハンス細胞学会
  • Place of Presentation: 京都
  • Year and Date: 2015-11-05
  • Int'l Joint Research
• [Presentation] Putative regulation of IgE production by antigen-specific cell aggregation. (2014)
  • Author(s): Hikosaka, M., Murata, A., Shimoda, Y., Yoshino, M., and Hayashi, S.I.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 国際会議場（京都）
  • Year and Date: 2014-12-11
• [Presentation] Migration of skin antigen-transporting cells in PYNOD-deficient mice. (2014)
  • Author(s): Yoshino, M., Imamura, R., Murata, A., Shimoda, Y., Hikosaka, M., Suda, T., and Hayashi, S.I.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 国際会議場（京都）
  • Year and Date: 2014-12-10
• [Presentation] Local increase of mast cells sustains after resolution of chronic contact dermatitis. (2014)
  • Author(s): Murata, A., Yoshino, M., Shimoda, Y., Hikosaka, M., and Hayashi, S.I.
  • Organizer: 第43回日本免疫学会学術集会
  • Place of Presentation: 国際会議場（京都）
  • Year and Date: 2014-12-10
• [Remarks] 鳥取大学医学部生命科学科免疫学分野
  • URL: http://www.med.tottori-u.ac.jp/immunol/5983.html
• [Remarks] 鳥取大学医学部生命科学科免疫学分野のホームページ
  • URL: http://www.med.tottori-u.ac.jp/immunol/5983.html