Function of extracellular ADP-ribosyl cyclase BST-1/CD157 in the intestine
| Project/Area Number |
26460499
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Kawasaki Medical School |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
矢作 綾野 川崎医科大学, 医学部, 助教 (10584873)
五十嵐 英哉 川崎医科大学, 医学部, 准教授 (40291538)
|
|---|
| Research Collaborator |
HIRANO Hiroyasu 川崎医科大学, 医学部, 大学院生
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 腸内細菌叢 / 無菌マウス / ADPリボシルシクラーゼ / 腸管内常在細菌叢 / メタボローム解析 / 1型胸腺非依存性抗原 / 無菌化(germ free) / BST-1/CD157 / 腸内常在細菌叢 / ノックアウトマウス |
|---|
| Outline of Final Research Achievements |
Bone marrow stromal cell antigen-1(BST-1)/CD157 is a GPI-anchored ectoenzyme having cyclase activity that converts NAD to cyclic ADP-ribose. BST-1 is now appreciated as an entero-neuro-immune regulator. Using the knock-out mice (KO) that lack CD157, CD38 or both, the functions of CD157 in the intestine was examined.The cells expressing CD157 or CD38 locate at distinct regions of the gut system.Decreased severity of DDS-induced colitis in CD157KO indicated that CD157 has a function to promote the colitis. Germ-free CD157KO exhibited the abnormal phenotypes in the cellular dynamics that had not been recognized in the SPF condition. T-RFLP analyses of the feces of double(D)KO revealed a reduced frequency of a certain Operational Taxonomic Unit in the microbiota compared with those of wild type mice. The metabolome analyses of the plasma of DKO revealed the changes in the amount of metabolites in the several pathways.
These data indicate that ADP-ribosyl cyclases regulates the microbiota.
|
Report
(4 results)
Research Products
(15 results)
-
-
[Journal Article] An immunohistochemical, enzymatic, and behavioral study of CD157/BST-1 as a neuroregulator.2017
Author(s)
Haruhiro Higashida, Mingkun Liang, Toru Yoshihara, Shirin Akther, Azam Fakhrul, Cherepanov Stanislav, Tae-Sik Nam, Uh-Hyun Kim, Satoka Kasai, Tomoko Nishimura, Naila Al Mahmuda, Shigeru Yokoyama, Katsuhiko Ishihara, Maria Gerasimenko, Alla Salmina, Jing Zhong, Takahiro Tsuji, Chiharu Tsuji and Olga Lopatina.
-
Journal Title
BMC Neuroscience
Volume: 18
Issue: 1
Pages: 35-35
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Lipo-oxytocin-1, A Novel Oxytocin Analog Conjugated with Two Palmitoyl Groups, Has Long-lasting Effects on Anxiety-related Behavior and Social Avoidance in CD157 Knockout Mice.2015
Author(s)
A. Mizuno, S. M. Cherepanov, Y. Kikuchi, A. Fakhrul, S. Akther, K. Deguchi, T. Yoshihara, K. Ishihara, S. Shuto , H. Higashida
-
Journal Title
Brain Sci
Volume: 5
Issue: 1
Pages: 3-13
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Anxiety- and depression-like behavior in mice lacking the CD157/BST1 gene, a risk factor for Parkinson's disease.2014
Author(s)
Lopatina O, Yoshihara T, Nishimura T, Zhong J, Akther S, Fakhrul AA, Liang M, Higashida C, Sumi K, Furuhara K, Inahata Y, Huang JJ, Koizumi K, Yokoyama S, Tsuji T, Petugina Y, Sumarokov A, Salmina AB, Hashida K, Kitao Y, Hori O, Asano M, Kitamura Y, Kozaka T, Shiba K, Zhong F, Xie MJ, Sato M, Ishihara K, Higashida H.
-
Journal Title
Front Behav Neurosci
Volume: 8
Pages: 1-18
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
