| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Echinococcus mitochondrial respiratory complexII contain unusual amino acid at very conserved site, which was Phe instead of common Ala. Same substitution was also found in bacteria Rhodospirillum rubrum. To asses the influence of this amino acid on catalysis, mutaion Ala to Phe and Phe to Ala was introduced into the complex II of Rhodobacter capsulatus and R. rubrum, respectively.
As a result, this mutation read to enhancement of Quinol-Fumarate reductase activities and not much change in Succinate-Quinone reductase activities. This amino acid has some roll on activity adjustment.
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