Proteome analysis of toxoplasmic retinochoroiditis-a quest of biomarker
Project/Area Number |
26460505
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Parasitology (including sanitary zoology)
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Research Institution | Chiba University |
Principal Investigator |
Norose Kazumi 千葉大学, 大学院医学研究院, 准教授 (30156244)
|
Co-Investigator(Kenkyū-buntansha) |
佐藤 守 千葉大学, 医学部附属病院, 特任准教授 (20401002)
日和佐 隆樹 千葉大学, 大学院医学研究院, 准教授 (30260251)
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Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | トキソプラズマ / トキソプラズマ性網脈絡膜炎 / プロテオーム解析 / バイマーカー / バイオマーカー / プロテオソーム / 前房水 / 硝子体液 / プロテオーム |
Outline of Final Research Achievements |
We established successfully a murine model of ocular toxoplasmosis (OT) infected perorally. Observations with fundus camera and fluorescein angiogram revealed that this model resembled with the human OT. The levels of cytokines and chemokines in aqueous humor, cerebrospinal fluid and serum were investigated and some molecules were identified to increase significantly after infection. In addition, some specific proteins were identified to either increase or decrease 2-fold after infection by the analysis on mouse retina using two-dimensional electrophoresis and mass spectrometry. It is suggestive that these molecules may play pivotal roles in the pathogenesis of OT and that analyzing these molecules is helpful to diagnose human OT during its early stage.
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Report
(4 results)
Research Products
(96 results)
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[Journal Article] Circulating anti-coatomer protein complex subunit epsilon (COPE) autoantibodies as a potential biomarker for cardio- and cerebro-vascular events in patients with obstructive sleep apnea2017
Author(s)
Matsumura T, Terada J, Kinoshita T, Sakurai Y, Yahaba M, Ema R, Amata A, Sakao S, Nagashima K, Tatsumi K, Hiwasa T
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Journal Title
J Clin Sleep Med
Volume: 13
Pages: 393-400
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Peer Reviewed / Int'l Joint Research
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[Journal Article] An anti-deoxyhypusine synthase antibody as a marker of atherosclerosis-related cerebral infarction, myocardial infarction, diabetes mellitus, and chronic kidney disease2017
Author(s)
Nakamura R, Tomiyoshi G, Shinmen N, Kuroda H, Kudo T, Doi H,, et al
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Journal Title
SM Atheroscler J
Volume: 1
Pages: 1001
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[Journal Article] Immune-complex level of cofilin-1 in sera is associated with cancer progression and poor prognosis in pancreatic cancer.2017
Author(s)
Satoh M, Takano S, Sogawa K, Noda K, Yoshitomi H, Ishibashi M, Mogushi K, Takizawa H, Otsuka M, Shimizu H, Miyazaki M, Nomura F.
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Journal Title
Cancer Sci
Volume: 印刷中
Pages: 印刷中
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Peer Reviewed
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[Journal Article] Clinical characteristics and computed tomography findings of pulmonary toxoplasmosis after hematopoietic stem cell transplantation.2016
Author(s)
Sumi M, Norose K, Hikosaka K, Kaiume H, Takeda W, Kirihara T, Kurihara T, Sato K, Ueki T, Hiroshima Y, Kuraishi H, Watanabe M, Kobayashi H.
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Journal Title
Int J Hematol
Volume: 104
Pages: 729-740
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Peer Reviewed
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[Journal Article] Anti-FIRs (PUF60) auto-antibodies are detected in the sera of early-stage colon cancer patients.2016
Author(s)
Kobayashi S, Hoshino T, Hiwasa T, Satoh M, Rahmutulla B, Tsuchida S, Komukai Y, Tanaka T, Matsubara H, Shimada H, Nomura F, Matsushita K
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Journal Title
Oncotarget
Volume: 7
Pages: 82493-82503
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Peer Reviewed / Int'l Joint Research
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[Journal Article] Enhancing analysis throughput, sensitivity and specificity in LC/ESI-MS/MS assay of plasma 25-hydroxyvitamin D3 by derivatization with triplex 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione (DAPTAD) isotopologues2016
Author(s)
Ogawa S, Kittaka H, Nakata A, Komatsu K, Sugiura T, Satoh M, Nomura F, Higashi T
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Journal Title
J Pharm Biomed Anal
Volume: 136
Pages: 126-133
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[Journal Article] Fibrinogen alpha C chain 5.9 kDa fragment (FIC5.9), a biomarker for various pathological conditions, is produced in post-blood collection by fibrinolysis and coagulation factors.2016
Author(s)
Kikuchi W, Nishimura M, Kuga T, Tsuchida S, Saito T, Satoh M, Noda K, Kodera Y, Tomonaga T, Nomura F
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Journal Title
Clin Proteomics
Volume: 13
Pages: 27
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[Journal Article] Identification of a novel serum biomarker for pancreatic cancer, C4b-binding protein α-chain (C4BPA) by quantitative proteomic analysis using tandem mass tags.2016
Author(s)
Sogawa K, Takano S, Iida F, Satoh M, Tsuchida S, Kawashima Y, Yoshitomi H, Sanda A, Kodera Y, Takizawa H, Mikata R, Ohtsuka M, Shimizu H, Miyazaki M, Yokosuka O, Nomura F
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Journal Title
Br J Cancer
Volume: 115
Pages: 949-956
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[Journal Article] Forensic study of Schistosoma japonicum eggs found in an autopsy case.2016
Author(s)
Motomura A, Norose K, Hoshioka Y, Torimitsu S, Chiba F, Makino Y, Inokuchi G, Yajima D, Ohta N, Kumagai T, Iwase H
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Journal Title
Parasitol Int
Volume: 65(3)
Pages: 285-287
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Peer Reviewed / Open Access
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[Journal Article] Elevated adiponectin antibody levels in sera of patients with atherosclerosis-related coronary artery disease, cerebral infarction, and diabetes mellitus.2016
Author(s)
Hiwasa T, Zhang XM, Kimura R, Ohno M, Chen PM, Nishi E, Ono K, Kimura T, Kamitsukasa I, Wada T, Aotsuka S, Mine S, Takizawa H, Kashiwado K, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Yokote K, Nakamura R, Tomiyoshi G, Shinmen N, Kuroda H
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Journal Title
J Circ Biomark
Volume: 5
Pages: 8
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[Journal Article] Identification of cerebral infarction-specific antibody markers from autoantibodies detected in patients with systemic lupus erythematosus.2015
Author(s)
Goto K, Sugiyama T, Matsumura R, Zhang XM, Kimura R, Taira A, Arita E, Iwase K, Kobayashi E, Iwadate Y, Saeki N, Mori M, Uzawa A, Muto M, Kuwabara S, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Sakurai K, Fujimoto M, Yokote K, Nakayama T, Harada J, Kobayashi Y, Ohno M, Chin H, ....., Hiwasa T.
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Journal Title
J Mol Biomark Diagnos
Volume: 6
Pages: 2
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Peer Reviewed / Open Access
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[Journal Article] Elevated serum antibody levels against cyclin L2 in patients with esophageal squamous cell carcinoma.2015
Author(s)
Shimada H, Ito M, Kagaya A, Shiratori T, Kuboshima M, Suzuki M, Liu TL, Nabeya Y, Matsubara H, Matsushita K, Nomura F, Takiguchi M, Hiwasa T
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Journal Title
J Cancer Sci Ther
Volume: 7(2)
Pages: 60-66
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Association of serum antibody levels against TUBB2C with diabetes and cerebral infarction.2015
Author(s)
Hiwasa T, Zhang XM, Kimura R, Machida T, Kitamura K, Yamazoe R, Kunimatsu M, Mine S, Kobayashi E, Iwadate Y, Saeki N, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Sakurai K, Fujimoto M, Yokote K, Iwata Y, Nakayama T, Harada J, Kobayashi Y, Ohno M, Chen PM, Nishi E, Yokota M, Kamitsukasa I, et al.
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Journal Title
Gratis J Biomed Sci
Volume: 1(2)
Pages: 49-63
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[Journal Article] Elevated levels of autoantibodies against ATP2B4 and BMP-1 in sera of patients with atherosclerosis-related diseases.2015
Author(s)
Hiwasa T, Machida T, Zhang XM, Kimura R, Wang H, Iwase K, Ashino H, Taira A, Arita E, Mine S, Ohno M, Chen PM, Nishi E, Kitamura K, Yamazoe R, Takizawa H, Kashiwado K, Kamitsukasa I, Wada T, Aotsuka A, Kobayashi E, Matsutani T, Iwadate Y, Saeki N, Mori M, Uzawa A, Muto M, Sugimoto K, Kuwabara S, Iwata Y, et al.
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Journal Title
Immunome Res
Volume: 11
Pages: 097
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[Journal Article] Successful treatment of toxoplasmic encephalitis rapidly diagnosed by a polymerase chain reaction after allogeneic hematopoietic stem cell transplantation: two cases reports and a review of the literature.2015
Author(s)
Miyagi T, Itonaga H, Aosai F, Taguchi J, Norose K, Mochizuki K, Fujii H, Furumoto A, Ohama M, Karimata K , Yamanoha A, Taniguchi H, Sato S , Taira N, Moriuchi Y, Fukushima T, Masuzaki H, Miyazaki Y.
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Journal Title
Transplant Infect Dis
Volume: 印刷中
Pages: 印刷中
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[Journal Article] Identification of Cerebral Infarction-Specific Antibody Markers from Autoantibodies Detected in Patients with Systemic Lupus Erythematosus.2015
Author(s)
Goto K, Sugiyama T, Matsumura R, Zhang XM, Kimura R, Taira A, Arita E, Iwase K, Kobayashi E, Iwadate Y, Saeki N, Mori M, Uzawa A, Muto M, Kuwabara S, Takemoto M, Kobayashi K, Kawamura H, Ishibashi R, Sakurai K, et al
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Journal Title
J. Mol. Biomark. Diagnos.
Volume: 6
Pages: 2
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Urinary albumin and transferrin as early diagnostic markers of chronic kidney disease.2015
Author(s)
Maeda H, Sogawa K, Sakaguchi K, Abe S, Sagizaka W, Mochizuki S, Horie W, Watanabe T, Shibata Y, Satoh M, Sanda A, Nomura F, Suzuki J
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Journal Title
J Vet Med Sci.
Volume: 印刷中
Pages: 印刷中
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[Journal Article] Haploinsufficiency of the c-myc transcriptional repressor FIR, as a dominant negative-alternative splicing model, promoted p53-dependent T-cell acute lymphoblastic leukemia progression by activating Notch12015
Author(s)
Matsushita K, Kitamura K, Rahmutulla B, Tanaka N, Ishige T, Satoh M, Hoshino T, Miyagi S, Mori T, Itoga S, Shimada H, Tomonaga T, Kito M, Nakajima-Takagi Y, Kubo S, Nakaseko C, Hatano M, Miki T, Matsuo M, Fukuyo M, Kaneda A, Iwama A, Nomura F
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Journal Title
Oncotarget.
Volume: 6
Pages: 5102-5117
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[Journal Article] Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis.2014
Author(s)
Ogita M, Tsuchida S, Aoki A, Satoh M, Kado S, Sawabe M, Nanbara H, Kobayashi H, Takeuchi Y, Mizutani K, Sasaki Y, Nomura F, Izumi Y
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Journal Title
Lasers Med Sci.
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[Journal Article] Moesin is a possible target molecule for cytomegalovirus-related Guillain-Barre syndrome.2014
Author(s)
Sawai S, Satoh M, Mori M, Misawa S, Sogawa K, Kazami T, Ishibashi M, Beppu M, Shibuya K, Ishige T, Sekiguchi Y, Noda K, Sato K, Matsushita K, Kodera Y, Nomura F, Kuwabara S
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Journal Title
Neurology.
Volume: 83
Pages: 113-117
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[Journal Article] Alternative splicing of FBP-interacting repressor coordinates c-Myc, P27Kip1/cyclinE and Ku86/XRCC5 expression as a molecular sensor for bleomycin-induced DNA damage pathway.2014
Author(s)
Rahmutulla B, Matsushita K, Satoh M, Seimiya M, Tsuchida S, Kubo S, Shimada H, Ohtsuka M, Miyazaki M, Nomura F.
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Journal Title
Oncotarget
Volume: 5
Pages: 2404-2417
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[Journal Article] Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis.2014
Author(s)
Segawa S, Sawai S, Murata S, Nishimura M, Beppu M, Sogawa K, Watanabe M, Satoh M, Matsutani T, Kobayashi M, Iwadate Y, Kuwabara S, Saeki N, Nomura F
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Journal Title
Clin Chim Acta
Volume: 435
Pages: 59-61
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[Presentation] 急性冠症候群におけるナルディライシンNardilycinの診断バイオマーカーとしての有用性の検討2016
Author(s)
大野美紀子, 陳博敏, 日和佐隆樹, 西清人, 西城さやか, 森田雄介, 松田真太郎, 桑原康秀, 尾野亘, 今井逸雄, 井上勝美, 村井達哉, 北徹, 木村剛, 西英一郎
Organizer
第39回日本分子生物学会
Place of Presentation
パシフィコ横浜 (神奈川県 横浜市)
Year and Date
2016-11-30 – 2016-12-02
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