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Larval proliferation mechanisms of pathogenic cestodes revealed by draft genome sequence of Sparganum proliferum.

Research Project

Project/Area Number 26460510
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionUniversity of Miyazaki

Principal Investigator

Maruyama Haruhiko  宮崎大学, 医学部, 教授 (90229625)

Co-Investigator(Kenkyū-buntansha) 菊地 泰生  宮崎大学, 医学部, 准教授 (20353659)
Co-Investigator(Renkei-kenkyūsha) KURAMOCHI TOSHIAKI  独立行政法人国立科学博物館, 動物研究部, 研究部長 (80277590)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords孤虫症 / 芽殖孤虫 / 条虫 / 比較ゲノム / プレロセルコイド / 病原性 / 寄生虫 / ゲノム / 遺伝子発現 / マンソン孤虫
Outline of Final Research Achievements

Sparganosis is caused by the plerocercoid of Pseudophyllidean tapeworms. While Spirometra spp. cause benign sparganosis, Sparganum proliferum causes fatal disease, in which plerocercoids proliferate continuously in humans, resulting in the death of the patient. We compared the genomes of S. proliferum and S. erinaceieuropaei, to understand the basis for S. proliferum pathogenicity.
We produced a 653 Mb assembly with N50 of 1.2 Mb for S. proliferum and a 796 Mb assemblies with N50 of 821 kb for Spirometra spp., respectively. In S. proliferum genome, 16 gene families were expanded and 26 families were contracted. Most of the expanded gene families did not have good similarities to known genes. Some of the contracted gene families were associated with organ development, signal transduction, and apoptotic processes. These changes in S. proliferum genome seem to reflect the activities of plerocercoids, which do not have developed organs and live in the stable environment.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2017 2016 2015 2014

All Journal Article (13 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 13 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Venestatin, a Ca++-binding protein from the parasitic nematode Strongyloides venezuelensis, is involved in the larval migration process2017

    • Author(s)
      Tsubokawa D, Hatta T, Kikuchi T, Maeda H, Mikami F, Alim MA, Maruyama H, Tsuji N
    • Journal Title

      Int J Parasitol.

      Volume: in press Issue: 8 Pages: 501-509

    • DOI

      10.1016/j.ijpara.2017.01.008

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Larva migrans syndrome caused by Toxocara and Ascaris roundworm infections in Japanese patients2016

    • Author(s)
      Yoshida A, Hombu A, Wang Z, Maruyama H
    • Journal Title

      Eur J Clin Microbiol Infect Dis

      Volume: 35 Issue: 9 Pages: 1521-1529

    • DOI

      10.1007/s10096-016-2693-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Optimal ELISA antigen for the diagnosis of Ascaris suum infection in humans2016

    • Author(s)
      Yoshida A, Kikuchi T, Nakagaki S, Maruyama H
    • Journal Title

      Parasitol Res

      Volume: 115 Pages: 4701-4705

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Venison, another source of Paragonimus westermani infection.2016

    • Author(s)
      Yoshida A, Matsuo K, Moribe J, Tanaka R, Kikuchi T, Nagayasu E, Misawa N, Maruyama H
    • Journal Title

      Parasitol Int

      Volume: 65 Issue: 6 Pages: 607-612

    • DOI

      10.1016/j.parint.2016.09.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-Wide Analyses of Individual Strongyloides stercoralis (Nematoda: Rhabditoidea) Provide Insights into Population Structure and Reproductive Life Cycles2016

    • Author(s)
      Kikuchi T, Hino A, Tanaka T, Aung MP, Afrin T, Nagayasu E, Tanaka R, Higashiarakawa M, Win KK, Hirata T, Htike WW, Fujita J, Maruyama H
    • Journal Title

      PLoS Negl Trop Dis

      Volume: 10 Issue: 12 Pages: e0005253-e0005253

    • DOI

      10.1371/journal.pntd.0005253

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The genomic basis of parasitism in the Strongyloides clade of nematodes2016

    • Author(s)
      Hunt, V. L. Tsai, I. J. Coghlan, A. Reid, A. J. Holroyd, N. Foth, B. J. Tracey, A. Cotton, J. A. Stanley, E. J. Beasley, H. Bennett, H. M. Brooks, K. Harsha, B. Kajitani, R. Kulkarni, A. Harbecke, D. Nagayasu, E et.al
    • Journal Title

      Nature genetics

      Volume: 48 Issue: 3 Pages: 299-307

    • DOI

      10.1038/ng.3495

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A novel method to assess the biodiversity of parasites using 18S rDNA Illumina sequencing; parasitome analysis method.2016

    • Author(s)
      Hino A, Maruyama H, Kikuchi T.
    • Journal Title

      Parasitol Int

      Volume: in press Issue: 5 Pages: 572-575

    • DOI

      10.1016/j.parint.2016.01.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits.2015

    • Author(s)
      Palomares-Rius JE, Tsai IJ, Karim N, Akiba M, Kato T, Maruyama H, Takeuchi Y, Kikuchi T.
    • Journal Title

      BMC Genomics

      Volume: 16 Issue: 1 Pages: 1845-1845

    • DOI

      10.1186/s12864-015-2085-0

    • NAID

      120005758216

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Optimal primaquine use for radical cure of Plasmodium vivax and Plasmodium ovale malaria in Japanese travelers--A retrospective analysis.2015

    • Author(s)
      Shimizu S, Kikuchi T, Koga M, Kato Y, Matsuoka H, Maruyama H, Kimura M
    • Journal Title

      Travel Med Infect Dis

      Volume: 13 Issue: 3 Pages: 235-240

    • DOI

      10.1016/j.tmaid.2014.11.005

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Paragonimiasis in Japan: A Twelve-year Retrospective Case Review (2001-2012)2015

    • Author(s)
      Nagayasu E, Yoshida A, Hombu A, Horii Y, Maruyama H
    • Journal Title

      Internal Medicine

      Volume: 54 Issue: 2 Pages: 179-186

    • DOI

      10.2169/internalmedicine.54.1733

    • NAID

      130004903022

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differential serodiagnostics of Toxocara canis and Toxocara cati - is it possible?2015

    • Author(s)
      Poulsen CS, Skov S, Yoshida A, Skallerup P, Maruyama H, Thamsborg SM, Nejsum P
    • Journal Title

      Parasite Immunol

      Volume: 37 Issue: 4 Pages: 204-207

    • DOI

      10.1111/pim.12181

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Karyotype and reproduction mode of the rodent parasite Strongyloides venezuelensis.2014

    • Author(s)
      Hino A, Tanaka T, Takaishi M, Fujii Y, Palomares-Rius JE, Hasegawa K, Maruyama H, Kikuchi T
    • Journal Title

      Parasitology

      Volume: 141 Issue: 13 Pages: 1736-1745

    • DOI

      10.1017/s0031182014001036

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Assessment of Helminth Biodiversity in Wild Rats Using 18S rDNA Based Metagenomics2014

    • Author(s)
      Ryusei Tanaka, Akina Hino, Isheng J. Tsai, Juan Emilio Palomares-Rius1, Ayako Yoshida,Yoshitoshi Ogura, Tetsuya Hayashi, Haruhiko Maruyama, Taisei Kikuchi
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 10 Pages: e110769-e110769

    • DOI

      10.1371/journal.pone.0110769

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ベネズエラ糞線虫における寄生関連遺伝子ファミリーの機能分化2016

    • Author(s)
      丸山治彦、前田安信、Shakhinur I. Mondal、長安英治、日野明紀菜、菊地泰生
    • Organizer
      日本寄生虫学会大会
    • Place of Presentation
      宮崎市
    • Year and Date
      2016-03-19
    • Related Report
      2015 Research-status Report
  • [Presentation] Secretome analysis of Strongyloides venezuelensis identified soluble and insoluble proteins related to parasitism of the nematode.2015

    • Author(s)
      Maeda Y, Palomares-Rius JE, Karim N, Nakatake A, Nagayasu E, Hino A, Kikuchi T, Maruyama H
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      三鷹市
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Assessment of helminth biodiversity in wild rats using 18S rDNA based metagenomics.2015

    • Author(s)
      田中龍聖、日野明紀菜、Tsai Isheng J, Palomares-Rius JE, 吉田彩子、小椋義俊、林哲也、丸山治彦、菊地泰生
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      三鷹市
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Prevalence and genetic diversity of Strongyloides stercoralis in Lower Myanmar.2015

    • Author(s)
      Nagayasu E, Myo Pa Pa Thet Hnin Htwe Aung, Hino A, Wa Win Htike, Maruyama H
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      三鷹市
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] ブタ回虫新規C-typeレクチン遺伝子のcDNAクローニングと発現解析2015

    • Author(s)
      吉田彩子、中垣しおり、菊地泰生、丸山治彦
    • Organizer
      第84回日本寄生虫学会大会
    • Place of Presentation
      三鷹市
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] Adaptation Strategy of Nematode to Parasitic Mode of Life; Genome Analysis of Strongyloides venezuelensis.2015

    • Author(s)
      Maruyama H, Kikuchi T, Tsai JI, Hayashi T, Ito T, Viney M, Berriman M
    • Organizer
      Expanding Frontiers of Genome Science II
    • Place of Presentation
      Tokyo
    • Year and Date
      2015-01-20 – 2015-01-21
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2018-03-22  

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