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A New nanoparticle glycogen for development of vaccine against malaria

Research Project

Project/Area Number 26460511
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionKagoshima University

Principal Investigator

Miyata Takeshi  鹿児島大学, 農水産獣医学域農学系, 准教授 (20448591)

Co-Investigator(Kenkyū-buntansha) 門川 淳一  鹿児島大学, 理工学域工学系, 教授 (30241722)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsマラリア / ワクチン / 伝搬阻止 / 三日熱マラリア原虫 / ナノ粒子 / ワクチンプラットフォーム
Outline of Final Research Achievements

We have developed a new technology to enhance immune responses by generating complex which composed DNA-binding protein fused vaccine antigen, DNA and nano-particle glycogen (NPG). In this system, NPG was used as scaffold material, DNA was used as crosslinker material for vaccine antigen and NPG. New tricomponent vaccine complex (DBP-Vaccine/DNA/NPG) function was evaluated using an ookinete surface protein of Plasmodium vivax, Pvs25. The tricomplex immunized mice were conferred a high immune response compared to Pvs25 alone or DBP-Pvs25/DNA complex immunized groups. This system may be a promising approach for development of subunit vaccines against malaria.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014

All Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (9 results) (of which Invited: 4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Fiber knob domain lacking the shaft sequence but fused to a coiled coil is a candidate subunit vaccine against egg-drop syndrome.2016

    • Author(s)
      Harakuni T, Andoh K, Sakamoto R, Tamaki Y, Miyata T, Uefuji H, Yamazaki K, Arakawa T.
    • Journal Title

      Vaccine

      Volume: 34 Issue: 27 Pages: 3184-3190

    • DOI

      10.1016/j.vaccine.2016.04.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control.2016

    • Author(s)
      Galay RL, Miyata T, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Methods in Molecular Biology

      Volume: 25 Pages: 16-23

    • DOI

      10.1007/978-1-4939-3389-1_18

    • ISBN
      9781493933884, 9781493933891
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Cholera toxin B subunit pentamer reassembled from Escherichia coli inclusion bodies for use in vaccination.2016

    • Author(s)
      Tamaki Y, Harakuni T, Yamaguchi R, Miyata T, Arakawa T
    • Journal Title

      Vaccine

      Volume: 34(10) Issue: 10 Pages: 1268-1274

    • DOI

      10.1016/j.vaccine.2016.01.034

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria.2016

    • Author(s)
      Maeda H, Miyata T, Kusakisako K, Galay RL, Talactac MR, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Developmental & Comparative Immunology

      Volume: 57 Pages: 38-47

    • DOI

      10.1016/j.dci.2015.12.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis2015

    • Author(s)
      Kusakisako K, Masatani T, Miyata T, Galay RL, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
    • Journal Title

      Insect Molecular Biology

      Volume: 25 Issue: 1 Pages: 16-23

    • DOI

      10.1111/imb.12193

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Preparation of pH-Responsive Amphoteric Glycogen Hydrogels by α-Glucan Phosphorylase-Catalyzed Successive Enzymatic Reactions2015

    • Author(s)
      Yusei Takata, Kazuya Yamamoto, Jun-ichi Kadokawa
    • Journal Title

      Macromolecular Chemistry and Physics

      Volume: 216 Issue: 13 Pages: 1415-1420

    • DOI

      10.1002/macp.201500151

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis2015

    • Author(s)
      Maeda H, Kurisu K, Miyata T, Kusakisako K, Galay RL, Rio TM, Mochizuki M, Fujisaki K, Tanaka T
    • Journal Title

      Parasitol. Res.

      Volume: in press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel antibiotic-delivery system by using ovotransferrin as targeting molecule.2014

    • Author(s)
      Ibrahim HR, Tatsumoto S, Hajime O, Immerseel FV, Raspoet R, Miyata T
    • Journal Title

      Eur. J. Pharm. Sci

      Volume: 7 Pages: 59-69

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation and comparison of the potential of two ferritins as anti-tick vaccine antigens against Haemaphysalis longicornis2014

    • Author(s)
      Galay RL, Miyata T, Umemiya-Shirafuji R, Maeda H, Kusakisako K, Mochizuki M, Fujisaki K, Tanaka T
    • Journal Title

      Parasit. Vectors

      Volume: 7 Pages: 482-490

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tricomponent complex loaded with a mosquito-stage antigen of the malaria parasite induces potent transmission-blocking immunity2014

    • Author(s)
      Arakawa T, Tsuboi T, Sattabongkot J, Sakao K, Torii M, Miyata T
    • Journal Title

      Clinical and Vaccine Immunology

      Volume: 21 Issue: 4 Pages: 561-569

    • DOI

      10.1128/cvi.00053-14

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tricomponent fusion complex comprising a viral antigen, a pentameric alpha-helical coiled-coil, and an immunoglobulin-binding domain as an effective antiviral vaccine.2014

    • Author(s)
      Arakawa T, Harakuni T, Miyata T, Tafuku S, Tadano M
    • Journal Title

      Vaccine

      Volume: 32 Issue: 7 Pages: 864-871

    • DOI

      10.1016/j.vaccine.2013.12.016

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 会合体を用いた新規ワクチンプラットフォームの創製2017

    • Author(s)
      宮田健
    • Organizer
      第27回繊維学会西部支部セミナー
    • Place of Presentation
      鹿児島大学(鹿児島県、鹿児島市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 鶏の産卵低下症候群(EDS)予防ワクチンの開発2015

    • Author(s)
      原國哲也、安藤清彦、坂元隆一、上藤洋敬、宮田健、山崎憲一、新川武
    • Organizer
      第19回日本ワクチン学会
    • Place of Presentation
      名鉄犬山ホテル(愛知県、名古屋市)
    • Year and Date
      2015-11-14
    • Related Report
      2015 Research-status Report
  • [Presentation] タンパク質集合系を利用した新しいワクチンプラットフォームの構築2015

    • Author(s)
      宮田健
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山ホテル(鹿児島県、鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] pH-応答性両性グリコーゲンゲルの酵素合成2015

    • Author(s)
      高田祐成・山元和哉・門川淳一
    • Organizer
      第64回高分子討論会
    • Place of Presentation
      東北大学仙台キャンパス(宮城県、仙台市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
  • [Presentation] アナログ基質を用いた酵素反応による新規糖鎖の精密合成2015

    • Author(s)
      門川淳一
    • Organizer
      第25回繊維学会西部支部セミナー
    • Place of Presentation
      琉球大学(沖縄県、西原 町)
    • Year and Date
      2015-02-06
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Enzymatic Synthesis of Novel Functional Oligo- and Polysaccharides by Phosphorylase Catalysis2014

    • Author(s)
      Jun-ichi Kadokawa
    • Organizer
      5th Annual World Gene Convention2015
    • Place of Presentation
      Hainan International Convention and Exhibition Center(海南、中国)
    • Year and Date
      2014-11-14
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] ナノ粒子グリコーゲン(NPG)を用いた新規マラリアワクチン開発の技術基盤構築2014

    • Author(s)
      柿本 麻美子、小森園 亮、坪井 敬文、Jetsumon Sattabongkot、新川 武、Ibrahim Hisham、門川 淳一、宮田 健
    • Organizer
      第19回日本フードファクター学会
    • Place of Presentation
      鹿児島大学(鹿児島県、鹿児島市)
    • Year and Date
      2014-11-08 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] The critical functions of ferritins in Haemaphysalis longicornis and their potential as target molecules for tick control2014

    • Author(s)
      Galay Remil Linggatong、白藤(梅宮)梨可、宮田 健、草木迫 浩大、前田 大輝、望月 雅美、藤崎 幸蔵、田仲 哲也
    • Organizer
      第157回日本獣医学学術集会
    • Place of Presentation
      北海道大学(北海道、札幌市)
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 耐熱性ホスホリラーゼによる両性多糖の酵素合成2014

    • Author(s)
      高田祐成、下吹越理子、山元和哉、門川淳一
    • Organizer
      第63回高分子学会年次大会
    • Place of Presentation
      名古屋国際会議場(愛知県、名古屋市)
    • Year and Date
      2014-05-28
    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 卵低下症候群(EDS)予防ワクチン2014

    • Inventor(s)
      宮田健、他7名
    • Industrial Property Rights Holder
      宮田健、他7名
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-087620
    • Filing Date
      2014-04-21
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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