Visualization of immune responses against merozoites malaria infection

• Project/Area Number: 26460512
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: University of the Ryukyus
• Principal Investigator: KISHIMOTO Hidehiro 琉球大学, 医学(系)研究科(研究院), 教授 (80251213)
• Co-Investigator(Kenkyū-buntansha): 李 長春 琉球大学, 医学部, 非常勤講師 (20457691)
• Co-Investigator(Renkei-kenkyūsha): KUBO Masato 東京理科大学, 生命医科学研究所, 教授 (40277281) ; WATANABE Hisami 新潟大学, 国際感染症教育研究センター, 特任教授 (50143756)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: マラリア感染防御 / 免疫応答 / T細胞 / 赤血球型マラリア / CD８＋T細胞 / 脾臓 / マラリア / 感染防御 / 免疫記憶 / ワクチン

Outline of Final Research Achievements

In this study, we investigated two questions; first, where dose immune-responses against to erythroid type malaria infection occur. Second, where establised memory T cells specific for the erythroid type malaria are mainteined. To visualize above questions, IFNγ-VENUS transgenic mice were infected with erythroid type malaria and analysed at various time points. Within 7 days, strong T cell response to erythroid type malaria is observed in the spleen whereas there is little immune responses found in other tissue i.e. lymphnodes, bonemarrows, liver. More than 60 days after infection, IFNγ-VENUS positive T cells are found in the spleen. These results indicated that the main tissue, in which immune-responses against to erythroid type malaria infection occur, revealed the spleen.

Research Products

• [Journal Article] Extra-luminal detection of assumed colonic tumor site by near-infrared laparoscopy. (2016)
  • Author(s): Zako T, Ito M, Hyodo H, Yoshimoto M, Watanabe M, Takemura H, Kishimoto H, Kaneko K, Soga K, Maeda M
  • Journal Title: Surg Endosc Volume: 9 Pages: 4153-4159
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 希土類ナノ粒子OTN近赤外蛍光プローブによるマウスin vivoイメージングの応用 (2016)
  • Author(s): 岸本 英博, 福田啓介, 竹下寛之, 曽我公平
  • Journal Title: バイオイメージング Volume: 25 Pages: 16-19
  • Peer Reviewed
• [Journal Article] Requirement of interleukin 7 signaling for anti-tumor immune response under lymphopenic conditions in a murine lung carcinoma model (2016)
  • Author(s): Suzuki T, Kishimoto H, Abe R.
  • Journal Title: Cancer Immunol. Immunother. Volume: 65 Issue: 3 Pages: 341-354
  • DOI: 10.1007/s00262-016-1808-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Falciparum Malaria Incidentally Pretreated with Azithromycin (2015)
  • Author(s): Shibahara D, Kinjo T, Nishiyama N, Kami W, Nabeya D, Haranaga S, Higa F, Tateyama M, Shinzato T, Toma H, Kishimoto H, Fujita J.
  • Journal Title: Internal Medicine Volume: 54 Issue: 19 Pages: 2513-2516
  • DOI: 10.2169/internalmedicine.54.5399
  • NAID: 130005101817
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extra-luminal detection of assumed colonic tumor site by near-infrared laparoscopy. (2015)
  • Author(s): Zako T, Ito M, Hyodo H, Yoshimoto M, Watanabe M, Takemura H, Kishimoto H, Kaneko K, Soga K and Maeda, M.
  • Journal Title: Surgical Endoscopy Volume: 29 Pages: 1-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An asymmetric antibody repertoire is shaped between plasmablasts and plasma cells after secondary immunization with (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin. (2015)
  • Author(s): Tashiro Y, Murakami A, Goizuka R, Shimizu T, Kishimoto H, Azuma T
  • Journal Title: Int Immunol Volume: 12 Pages: 609-20
  • DOI: 10.1093/intimm/dxv040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] VHH提示ファージライブラリーからの製品化を見据えた抗体作製法 (2017)
  • Author(s): 村上明一、塚原成俊、吉田麻衣子、與那嶺周平、岸本英博
  • Organizer: 第８回バイオメディカルインターフェイス・ワークショップ
  • Place of Presentation: 宮古島市中央公民館（沖縄県宮古島市）
  • Year and Date: 2017-03-09
• [Presentation] The infusion of adipose tissue-derived mesenchymal stem cells controls bone marrow aplasia accompanied with graft-versus-host disease in a mouse model Yukiko Nishi, Akikazu Murakami, Hidehiro Kishimot (2016)
  • Author(s): Yukiko Nishi, Akikazu Murakami, Hidehiro Kishimoto, Yuko Murayama, Sawako Nakachi, Satoko Morishima, Hiroaki Masuzaki
  • Organizer: 第45回日本免疫学会総会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] 人工合成VHHファージディスプレイライブラリーから高い変性耐性を有するVHHクローンの単離と解析 (2016)
  • Author(s): 村上明一、吉田麻衣子、塚原成俊、岸本英博
  • Organizer: 第39回日本分子生物学会
  • Place of Presentation: Pacifico横浜（神奈川県横浜市）
  • Year and Date: 2016-12-02
• [Presentation] Rapid isolation of useful antibodies from semi-synthetic Alpaca VHH phage display libraries (2016)
  • Author(s): Murakami A,Yoshida M, Azuma T, Nakayama H, Muraoka J, Kishimoto H
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 東北大学川内北キャンパス（宮城県仙台市）
  • Year and Date: 2016-09-26
• [Presentation] Isolation and characterization of target antigen specific VHHs from unique semi-synthetic alpaca VHH phage display libraries (2016)
  • Author(s): Murakami A,Yoshida M, Azuma T, Nakayama H, Muraoka J, Kishimoto H
  • Organizer: 16th International congress of Immunology
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 2016-08-12
  • Int'l Joint Research
• [Presentation] ユニークな戦略で多様性を創製したsemi-syntheticアルパカVHHファージディスプレイライブラリーからの標的抗原結合性クローンの単離と解析 (2015)
  • Author(s): Murakami A, Yoshida M, Azuma T, Nakayama H, Muraoka J, Kishimoto H
  • Organizer: 第38回日本分子生物学会/第88回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] The generation of multimeric and highly effective cytokines (2015)
  • Author(s): Tsukahara N, Nakayama M, Murakami A, Nishimura M, Azuma T, Kishimoto H
  • Organizer: 第44回 日本免疫学会総会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] An asymmetric antibody repertoire is shaped between plasmablast and plasma cells after secondary immunization with (4-hydroxy-3-nitrophenyl)acetyl chicken γ-globulin. (2015)
  • Author(s): Tashiro Y, Murakami A, Goitsuka R, Shimizu T, Kishimoto H, Azuma T
  • Organizer: 第44回 日本免疫学会総会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2015-11-18
• [Presentation] 3-(1-ナフトイル)インドール誘導体に対する新規モノクローナル抗体の開発 (2015)
  • Author(s): 中山浩、村岡仁、村上明一、岸本英博
  • Organizer: 日本分析化学会第64年会
  • Place of Presentation: 九州大学伊都キャンパス（福岡県福岡市）
  • Year and Date: 2015-09-09
• [Presentation] The generation of multimeric and highly active cytokine-Fc fusion protein (2015)
  • Author(s): Murakami A, Kishimoto H
  • Organizer: The 15th Annual Meeting of the Federation of Clinical Immunology Societies
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2015-06-24
  • Int'l Joint Research