Development of a rapid diagnostic test for amebiasis by using fluorescent nanoparticles
| Project/Area Number |
26460516
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Tokai University |
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Principal Investigator |
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| Research Collaborator |
CHENG Xunjia 東海大学, 医学部, 客員教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 原虫感染症 / 赤痢アメーバ / 診断 / ナノ材料 |
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| Outline of Final Research Achievements |
Immunochromatographic assay kit using fluorescent silica nanoparticles was developed for rapid serodiagnosis of amebiasis. C-terminal fragment of intermediate subunit from Entamoeba histolytica surface lectin (Igl) was prepared in Escherichia coli and used as antigen. When the kit was evaluated by human monoclonal antibody specific to Igl, the lower detection limit was 100 pg. High sensitivity and high specificity of the kit were demonstrated by evaluation using serum samples from patients.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Identification of Entamoeba polecki with unique 18S rRNA gene sequences from Celebes crested macaques and pigs in Tangkoko Nature Reserve, North Sulawesi, Indonesia.2016
Author(s)
Tuda, J., Feng, M., Imada, M., Kobayashi, S., Cheng, X. and Tachibana, H.
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Journal Title
J. Euk. Microbiol.
Volume: 63
Issue: 5
Pages: 1-6
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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