Development of a rapid diagnostic test for amebiasis by using fluorescent nanoparticles

• Project/Area Number: 26460516
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Tokai University
• Principal Investigator: TACHIBANA Hiroshi 東海大学, 医学部, 教授 (10147168)
• Research Collaborator: CHENG Xunjia 東海大学, 医学部, 客員教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 原虫感染症 / 赤痢アメーバ / 診断 / ナノ材料

Outline of Final Research Achievements

Immunochromatographic assay kit using fluorescent silica nanoparticles was developed for rapid serodiagnosis of amebiasis. C-terminal fragment of intermediate subunit from Entamoeba histolytica surface lectin (Igl) was prepared in Escherichia coli and used as antigen. When the kit was evaluated by human monoclonal antibody specific to Igl, the lower detection limit was 100 pg. High sensitivity and high specificity of the kit were demonstrated by evaluation using serum samples from patients.

Research Products

• [Int'l Joint Research] 復旦大学(中国)
• [Int'l Joint Research] 復旦大学(中国)
• [Journal Article] Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant. (2017)
  • Author(s): Kazama M, Ogiwara S, Makiuchi T, Yoshida K, Nakada-Tsukui K, Nozaki T, Tachibana H
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 44273-44273
  • DOI: 10.1038/srep44273
  • Peer Reviewed / Open Access
• [Journal Article] 人獣共通感染症としてのアメーバ症 (2016)
  • Author(s): 橘 裕司、小林正規
  • Journal Title: 病原微生物検出情報 Volume: 37 Pages: 249-250
  • Open Access
• [Journal Article] Identification of Entamoeba polecki with unique 18S rRNA gene sequences from Celebes crested macaques and pigs in Tangkoko Nature Reserve, North Sulawesi, Indonesia. (2016)
  • Author(s): Tuda, J., Feng, M., Imada, M., Kobayashi, S., Cheng, X. and Tachibana, H.
  • Journal Title: J. Euk. Microbiol. Volume: 63 Issue: 5 Pages: 1-6
  • DOI: 10.1111/jeu.12304
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] IUD使用者で認めた歯肉アメーバと放線菌の子宮内感染から生じた腹腔内膿瘍の１例 (2016)
  • Author(s): 横田恭子、渡邊直樹、根ヶ山清、真嶋允人、嶌村将志、伊吹英美、串田吉生、橘 裕司、新井明治
  • Journal Title: Clin. Parasitol. Volume: 27 Pages: 93-95
• [Journal Article] Evaluation of the C-terminal fragment of Entamoeba histolytica Gal/GalNAc lectin intermediate subunit as a vaccine candidate against amebic liver abscess (2016)
  • Author(s): Min X, Feng M, Guan Y, Man S, Fu Y, Cheng X, Tachibana H.
  • Journal Title: PLoS Neglected Tropical Diseases Volume: 10
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Isolation and molecular characterization of Entamoeba nuttalli strains showing novel isoenzyme patterns from wild toque macaques in Sri Lanka (2016)
  • Author(s): Tachibana H, Yanagi T, Feng M, Bandara KB, Kobayashi S, Cheng X, Hirayama K, Rajapakse RP.
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 63 Issue: 2 Pages: 171-180
  • DOI: 10.1111/jeu.12265
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Entamoeba histolyticaとEntamoeba disparのIglレクチンの活性比較研究 (2016)
  • Author(s): 加藤健太郎、牧内貴志、橘 裕司
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-12-01
• [Presentation] 赤痢アメーバにおけるペルオキシレドキシンの局在と機能 (2016)
  • Author(s): 吉田和弘、牧内貴志、風間 真、福西菜穂子、橘 裕司
  • Organizer: 第49回日本原生生物学会大会
  • Place of Presentation: 岡山大学津島キャンパス（岡山県岡山市）
  • Year and Date: 2016-10-09
• [Presentation] Entamoeba histolyticaとEntamoeba disparレクチンの活性比較研究 (2016)
  • Author(s): 加藤健太郎、橘 裕司
  • Organizer: 第35回日本糖質学会年会
  • Place of Presentation: 高知市文化プラザかるぽーと（高知県高知市）
  • Year and Date: 2016-09-03
• [Presentation] IUD使用者で認めた歯肉アメーバと放線菌の子宮内感染から生じた腹腔内膿瘍の１例 (2016)
  • Author(s): 横田恭子、渡邊直樹、根ヶ山清、真嶋允人、嶌村将志、伊吹英美、串田吉生、橘 裕司、新井明治
  • Organizer: 第27回日本臨床寄生虫学会大会
  • Place of Presentation: 石川県政記念しいのき迎賓館（石川県金沢市）
  • Year and Date: 2016-06-18
• [Presentation] 蛍光シリカナノ粒子を用いたイムノクロマト法による赤痢アメーバ症迅速血清診断法の開発 (2016)
  • Author(s): 橘 裕司、福嶋將行、垣野あずみ、風間 真、牧内貴志、馮 萌、三好一富、増田剛太、大久保典雄、程 訓佳
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市民プラザ（宮崎県宮崎市）
  • Year and Date: 2016-03-20