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Development of a rapid diagnostic test for amebiasis by using fluorescent nanoparticles

Research Project

Project/Area Number 26460516
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Parasitology (including sanitary zoology)
Research InstitutionTokai University

Principal Investigator

TACHIBANA Hiroshi  東海大学, 医学部, 教授 (10147168)

Research Collaborator CHENG Xunjia  東海大学, 医学部, 客員教授
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords原虫感染症 / 赤痢アメーバ / 診断 / ナノ材料
Outline of Final Research Achievements

Immunochromatographic assay kit using fluorescent silica nanoparticles was developed for rapid serodiagnosis of amebiasis. C-terminal fragment of intermediate subunit from Entamoeba histolytica surface lectin (Igl) was prepared in Escherichia coli and used as antigen. When the kit was evaluated by human monoclonal antibody specific to Igl, the lower detection limit was 100 pg. High sensitivity and high specificity of the kit were demonstrated by evaluation using serum samples from patients.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 4 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (5 results)

  • [Int'l Joint Research] 復旦大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 復旦大学(中国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Behavior of DNA-lacking mitochondria in Entamoeba histolytica revealed by organelle transplant.2017

    • Author(s)
      Kazama M, Ogiwara S, Makiuchi T, Yoshida K, Nakada-Tsukui K, Nozaki T, Tachibana H
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 44273-44273

    • DOI

      10.1038/srep44273

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 人獣共通感染症としてのアメーバ症2016

    • Author(s)
      橘 裕司、小林正規
    • Journal Title

      病原微生物検出情報

      Volume: 37 Pages: 249-250

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] Identification of Entamoeba polecki with unique 18S rRNA gene sequences from Celebes crested macaques and pigs in Tangkoko Nature Reserve, North Sulawesi, Indonesia.2016

    • Author(s)
      Tuda, J., Feng, M., Imada, M., Kobayashi, S., Cheng, X. and Tachibana, H.
    • Journal Title

      J. Euk. Microbiol.

      Volume: 63 Issue: 5 Pages: 1-6

    • DOI

      10.1111/jeu.12304

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] IUD使用者で認めた歯肉アメーバと放線菌の子宮内感染から生じた腹腔内膿瘍の1例2016

    • Author(s)
      横田恭子、渡邊直樹、根ヶ山清、真嶋允人、嶌村将志、伊吹英美、串田吉生、橘 裕司、新井明治
    • Journal Title

      Clin. Parasitol.

      Volume: 27 Pages: 93-95

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Evaluation of the C-terminal fragment of Entamoeba histolytica Gal/GalNAc lectin intermediate subunit as a vaccine candidate against amebic liver abscess2016

    • Author(s)
      Min X, Feng M, Guan Y, Man S, Fu Y, Cheng X, Tachibana H.
    • Journal Title

      PLoS Neglected Tropical Diseases

      Volume: 10

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Isolation and molecular characterization of Entamoeba nuttalli strains showing novel isoenzyme patterns from wild toque macaques in Sri Lanka2016

    • Author(s)
      Tachibana H, Yanagi T, Feng M, Bandara KB, Kobayashi S, Cheng X, Hirayama K, Rajapakse RP.
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: 63 Issue: 2 Pages: 171-180

    • DOI

      10.1111/jeu.12265

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Entamoeba histolyticaとEntamoeba disparのIglレクチンの活性比較研究2016

    • Author(s)
      加藤健太郎、牧内貴志、橘 裕司
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 赤痢アメーバにおけるペルオキシレドキシンの局在と機能2016

    • Author(s)
      吉田和弘、牧内貴志、風間 真、福西菜穂子、橘 裕司
    • Organizer
      第49回日本原生生物学会大会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県岡山市)
    • Year and Date
      2016-10-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Entamoeba histolyticaとEntamoeba disparレクチンの活性比較研究2016

    • Author(s)
      加藤健太郎、橘 裕司
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザかるぽーと(高知県高知市)
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] IUD使用者で認めた歯肉アメーバと放線菌の子宮内感染から生じた腹腔内膿瘍の1例2016

    • Author(s)
      横田恭子、渡邊直樹、根ヶ山清、真嶋允人、嶌村将志、伊吹英美、串田吉生、橘 裕司、新井明治
    • Organizer
      第27回日本臨床寄生虫学会大会
    • Place of Presentation
      石川県政記念しいのき迎賓館(石川県金沢市)
    • Year and Date
      2016-06-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] 蛍光シリカナノ粒子を用いたイムノクロマト法による赤痢アメーバ症迅速血清診断法の開発2016

    • Author(s)
      橘 裕司、福嶋將行、垣野あずみ、風間 真、牧内貴志、馮 萌、三好一富、増田剛太、大久保典雄、程 訓佳
    • Organizer
      第85回日本寄生虫学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県宮崎市)
    • Year and Date
      2016-03-20
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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