Elucidation of autophagy evasion mechanism of Staphylococcus aureus by toxic shock syndrome toxin-1
| Project/Area Number |
26460517
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
Asano Krisana 弘前大学, 医学研究科, 講師 (70598622)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANE AKIO 弘前大学, 医学(系)研究科(研究院), 教授 (30164239)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 黄色ブドウ球菌 / TSST-1 / オートファジー |
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| Outline of Final Research Achievements |
Regarding the intracellular survival of S. aureus, we reported that toxic shock syndrome toxin-1 (TSST-1) suppresses autophagy in a superantigenic activity-independent manner. In this study, we further analyzed autophagy suppressing mechanism by TSST-1. In vitro
study revealed that TSST-1 interacts with one of Septin family proteins in the host cells. Interaction of TSST-1 promotes function of this target protein to regulate the autophagy, which
then leads to suppression of autophagosome formation and decrease of the bacterial number in the host cells. For in vivo analysis, S. aureus harboring superantigen activity-deficient mutant of TSST-1 (mTSST-1) was constructed. Autophagy - suppressing activity of this S. aureus strain was confirmed. We plan to analyze the mechanism using the mouse genetic modification model in the future.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] The emetic activity of staphylococcal enterotoxins, SEK, SEL, SEM, SEN and SEO, in house musk shrew (Suncus murinus).2017
Author(s)
Ono, H. K., Hirose, S., Naito, I., Sato’o, Y., Asano, K., Hu, D.-L., Omoe, K., Nakane, A.
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Journal Title
Microbiol. Immun.
Volume: 61
Issue: 1
Pages: 12-16
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Identification and characterization of a novel staphylococcal emetic toxin2015
Author(s)
Ono HK, Sato'o Y, Narita K, Naito I, Hirose S, Hisatsune J, Asano K, Hu DL, Omoe K, Sugai M, Nakane A.
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Journal Title
Appl Environ Microbiol
Volume: 81
Issue: 20
Pages: 7034-7040
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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