| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Regarding the intracellular survival of S. aureus, we reported that toxic shock syndrome toxin-1 (TSST-1) suppresses autophagy in a superantigenic activity-independent manner. In this study, we further analyzed autophagy suppressing mechanism by TSST-1. In vitro
study revealed that TSST-1 interacts with one of Septin family proteins in the host cells. Interaction of TSST-1 promotes function of this target protein to regulate the autophagy, which
then leads to suppression of autophagosome formation and decrease of the bacterial number in the host cells. For in vivo analysis, S. aureus harboring superantigen activity-deficient mutant of TSST-1 (mTSST-1) was constructed. Autophagy - suppressing activity of this S. aureus strain was confirmed. We plan to analyze the mechanism using the mouse genetic modification model in the future.
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