| Project/Area Number |
26460520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Gifu University |
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Principal Investigator |
GOTO Takatsugu 岐阜大学, 生命科学総合研究支援センター, 助教 (80326355)
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| Co-Investigator(Kenkyū-buntansha) |
田中 香お里 岐阜大学, 生命科学総合研究支援センター, 教授 (20242729)
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| Co-Investigator(Renkei-kenkyūsha) |
MORITA Yuji 愛知学院大学, 薬学部, 准教授 (00454322)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Bacteroides fragilis / カルバペネム耐性菌 / 中等度耐性株 / ゲノム DNA ライブラリー / MFS トランスポーター / 薬剤耐性機構 / 全ゲノム解析 / カルバペネム耐性 / 中等度耐性 / ゲノムDNAライブラリー / MFS transporter / 薬剤排出ポンプ / 嫌気性菌 |
|---|
| Outline of Final Research Achievements |
Carbamenem-resistant Bacteroides fragilis often makes treatment difficult. We aimed to identify a novel carbamenem-intermediate resistance factor using a genomic DNA library of the B. fragilis GAI92214 strain. We identified a major facilitator superfamily transporter that may contribute slightly to meropenem resistance in the GAI92214 strain. However, we could not fully explain carbamenem-intermediate resistance mechanism from this transporter alone. Thus, we started complete genome sequencing project of the GAI92214 strain. To date, we determined approximately 99% of the genomic sequence encoding about 4723 ORFs.
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