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The analysis of Haemophilus influenzae in alveolar epithelium cells and cytotoxic T-cell responses against the bacteria

Research Project

Project/Area Number 26460521
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bacteriology (including mycology)
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Nagata Toshi  浜松医科大学, 医学部, 教授 (90275024)

Co-Investigator(Kenkyū-buntansha) 榎本 紀之  浜松医科大学, 医学部附属病院, 講師 (50436961)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsインフルエンザ菌 / 気道上皮細胞 / ビトロネクチン / プロテインE
Outline of Final Research Achievements

We confirmed that some strains of NTHi (Nontypeable Haemophilus influenzae) invade human bronchial epithelial cells (BEAS-2B cell line) and studied the molecular mechanisms of the invasion. The results of our study showed NTHi attached on vitronectin which exists on bronchial epithelial cells. The attachment was inhibited with pretreatment of the peptide derived from 84-108 amino acid residues of NTHi protein E. The degree of the inhibition depended on the concentration of the peptide added. In addition, NTHi invasion into bronchial epithelial cells was also inhibited in the peptide concentration-dependent manner. These results indicate that the invasion of NTHi into bronchial epithelial cells is a process in which both vitronectin on the epithelial cells and NTHi protein E are involved.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (4 results)

All 2016 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells2015

    • Author(s)
      Ikeda M, Enomoto N, Hashimoto D, Fujisawa T, Inui N, Nakamura Y, Suda T
    • Journal Title

      BMC Microbiol

      Volume: 15 Issue: 1 Pages: 263-263

    • DOI

      10.1186/s12866-015-0600-8

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 非莢膜型インフルエンザ菌の気道上皮細胞侵入機構の解析2016

    • Author(s)
      池田政輝、榎本紀之、須田隆文、永田 年
    • Organizer
      第98回日本細菌学会関東支部総会
    • Place of Presentation
      東京歯科大学(東京)
    • Year and Date
      2016-10-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Nontypeable Haemophillus Influenzae Invades Bronchial Epithelial Cells Via Protein E And Vitronectin2015

    • Author(s)
      池田政輝
    • Organizer
      American Throacic Society (ATS) 2015
    • Place of Presentation
      デンバー
    • Year and Date
      2015-05-15 – 2015-05-20
    • Related Report
      2014 Research-status Report
  • [Presentation] Analysis of mechanisms by which nontypeable Haemophillus influenzae strains invade bronchial epithelial cells2015

    • Author(s)
      池田政輝
    • Organizer
      第55回日本呼吸器学会学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2015-04-17 – 2015-04-19
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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