The analysis of Haemophilus influenzae in alveolar epithelium cells and cytotoxic T-cell responses against the bacteria

• Project/Area Number: 26460521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Nagata Toshi 浜松医科大学, 医学部, 教授 (90275024)
• Co-Investigator(Kenkyū-buntansha): 榎本 紀之 浜松医科大学, 医学部附属病院, 講師 (50436961)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: インフルエンザ菌 / 気道上皮細胞 / ビトロネクチン / プロテインE

Outline of Final Research Achievements

We confirmed that some strains of NTHi (Nontypeable Haemophilus influenzae) invade human bronchial epithelial cells (BEAS-2B cell line) and studied the molecular mechanisms of the invasion. The results of our study showed NTHi attached on vitronectin which exists on bronchial epithelial cells. The attachment was inhibited with pretreatment of the peptide derived from 84-108 amino acid residues of NTHi protein E. The degree of the inhibition depended on the concentration of the peptide added. In addition, NTHi invasion into bronchial epithelial cells was also inhibited in the peptide concentration-dependent manner. These results indicate that the invasion of NTHi into bronchial epithelial cells is a process in which both vitronectin on the epithelial cells and NTHi protein E are involved.

Research Products

• [Journal Article] Nontypeable Haemophilus influenzae exploits the interaction between protein-E and vitronectin for the adherence and invasion to bronchial epithelial cells (2015)
  • Author(s): Ikeda M, Enomoto N, Hashimoto D, Fujisawa T, Inui N, Nakamura Y, Suda T
  • Journal Title: BMC Microbiol Volume: 15 Issue: 1 Pages: 263-263
  • DOI: 10.1186/s12866-015-0600-8
  • Peer Reviewed / Open Access
• [Presentation] 非莢膜型インフルエンザ菌の気道上皮細胞侵入機構の解析 (2016)
  • Author(s): 池田政輝、榎本紀之、須田隆文、永田 年
  • Organizer: 第98回日本細菌学会関東支部総会
  • Place of Presentation: 東京歯科大学（東京）
  • Year and Date: 2016-10-29
• [Presentation] Nontypeable Haemophillus Influenzae Invades Bronchial Epithelial Cells Via Protein E And Vitronectin (2015)
  • Author(s): 池田政輝
  • Organizer: American Throacic Society (ATS) 2015
  • Place of Presentation: デンバー
  • Year and Date: 2015-05-15 – 2015-05-20
• [Presentation] Analysis of mechanisms by which nontypeable Haemophillus influenzae strains invade bronchial epithelial cells (2015)
  • Author(s): 池田政輝
  • Organizer: 第55回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2015-04-17 – 2015-04-19