Membrane trafficking regulating LPS-induced autophagy

• Project/Area Number: 26460522
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: 公益財団法人結核予防会 結核研究所 (2015-2016); Hamamatsu University School of Medicine (2014)
• Principal Investigator: Seto Shintaro 公益財団法人結核予防会 結核研究所, 生体防御部 免疫科, 研究員(移行) (50383203)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: オートファジー / LPS / Rab GTPase / レジオネラ菌 / エフェクタータンパク質 / 小胞輸送機構 / 結核菌

Outline of Final Research Achievements

We screened Rab GTPase for regulators of LPS-induced autophagy. We have already identified that Rab39a binds to Beclin-1, PI3 kinase regulator and negatively regulates LPS-induce autophagy In this study, we found that Rab26 binds to Beclin-1 by immunoprecipitation analysis. Fluorescence microscopy analysis revealed that Rab26 localizes to lysosomes. Knock-down analysis showed that Rab26 negatively LPS-induced autophagy.

Research Products

• [Journal Article] M2 polarization of murine peritoneal macrophages induces regulatory cytokine production and suppresses T-cell proliferation. (2016)
  • Author(s): Shinji Oishi, Ken Sugimoto, et al.
  • Journal Title: Immunology Volume: 149 Issue: 3 Pages: 320-328
  • DOI: 10.1111/imm.12647
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 結核菌ファゴソームの分子解剖 (2014)
  • Author(s): 瀬戸真太郎、永田 年、堀井俊伸、小出幸夫
  • Journal Title: 日本細菌学雑誌 Volume: 63 Pages: 513-525
  • NAID: 130004688030
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] プロテオミクス解析による（多剤耐性）結核症、およびMycobacterium avium complex症感染組織における特異的分子マーカーの探索 (2017)
  • Author(s): 瀬戸真太郎, 森本耕三, 吉田 勤, 土方美奈子, 松下育美, 白石祐治, 倉島篤行, 慶長直人
  • Organizer: 第92回日本結核病学会総会
  • Place of Presentation: 東京
  • Year and Date: 2017-03-23
• [Presentation] プロテオミクス解析による肺MAC症肉芽腫の分子解剖 (2017)
  • Author(s): 瀬戸真太郎, 森本耕三, 吉田 勤, 土方美奈子, 松下育美, 白石祐治, 永田 年, 倉島篤行, 慶長直人
  • Organizer: 第90回日本細菌学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-19
• [Presentation] レジオネラ菌の新規エフェクタータンパク質とその標的宿主タンパク質の網羅的探索 (2016)
  • Author(s): 瀬戸真太郎、永田 年
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2016-03-23
• [Presentation] Mycobacterium tuberculosis escapes from autophagic target by hijacking the function of the host actin binding protein Coronin-1a. (2016)
  • Author(s): Shintaro Seto, Toshi Nagata, Yukio Koide
  • Organizer: 50th US-Japan Cooperative Medical Science Program
  • Place of Presentation: Bethesda
  • Year and Date: 2016-01-13
  • Int'l Joint Research
• [Presentation] マクロファージの小胞輸送機構からひもとく結核菌寄生戦略 (2015)
  • Author(s): 瀬戸真太郎
  • Organizer: 第89回日本感染症学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2015-04-16
• [Presentation] 結核の新規病原因子の解明 (2015)
  • Author(s): 瀬戸真太郎、小出幸夫
  • Organizer: 第90回日本結核病学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2015-03-26
• [Presentation] Host-Pathogen Interactionの観点から考え得る新しい治療法 (2015)
  • Author(s): 瀬戸真太郎、小出幸夫
  • Organizer: 第90回日本結核病学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2015-03-26