Regulation of bacterial pathogenicity by the ABC transporter

• Project/Area Number: 26460524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: Nishino Mitsuko 大阪大学, 産業科学研究所, 助教 (30510440)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細菌 / 多剤耐性 / 病原性 / トランスポーター / 異物排出 / 薬剤排出 / サルモネラ / MacAB / 異物 / 排出 / ABC / ATP / Mac / マクロライド / 感染症 / ABC型 / 薬剤耐性 / 宿主相互作用 / 大腸菌 / 緑膿菌

Outline of Final Research Achievements

Xenobiotic transporter contributes to bacterial multidrug resistance by expelling antibacterial drugs. However, physiological roles of most of these transporters remain unknown. In this study, we focused on the ABC type transporter MacAB, and conducted physiological function analysis. There were findings that transporters were related with the bacterial attachment to the host cell surface, involved in resistance to oxidative stress, involved in maintenance of biofilm formation, and partly in bacterial survival in the intestine. In addition, inhibitor candidates of transporters were selected by the screening. In the situation where the physiological functions of many transporters are unknown, these results clarify their physiological functions and these are important information useful for the development of new drugs.

Research Products

• [Journal Article] Phenotype microarray analysis of the drug efflux systems in Salmonella enterica serovar Typhimurium (2016)
  • Author(s): Yamasaki S, Fujioka T, Hayashi K, Yamasaki S, Hayashi-Nishino M, Nishino K
  • Journal Title: J Infect Chemother Volume: in press Issue: 11 Pages: 780-784
  • DOI: 10.1016/j.jiac.2016.03.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Multidrug efflux pumps contribute to Escherichia coli biofilm maintenance (2015)
  • Author(s): Seiji Yamasaki, Li-Yuan Wang, Takahiro Hirata, Mitsuko Hayashi-Nishino, Kunihiko Nishino
  • Journal Title: International Journal of Antimicrobial Agents Volume: 45 Issue: 4 Pages: 439-441
  • DOI: 10.1016/j.ijantimicag.2014.12.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of methylglyoxal on multidrug-resistant Pseudomonas aeruginosa. (2014)
  • Author(s): Katsuhiko Hayashi, Aiko Fukushima, Mitsuko Hayashi-Nishino, and Kunihiko Nishino
  • Journal Title: frontiers in Microbiology Volume: 5 Pages: 180-180
  • DOI: 10.3389/fmicb.2014.00180
  • Peer Reviewed / Open Access
• [Presentation] Development of Novel Therapeutic Strategies to Tackle Multidrug-Resistant Pathogens (2016)
  • Author(s): Mitsuko Hayashi-Nishino, Yoshimi Matsumoto and Kunihiko Nishino
  • Organizer: International Symposium Salmonella and Salmonellosis
  • Place of Presentation: Saint-Malo, France
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] Electron/immuno-electron tomography of autophagosomal membranes and bacterial multidrug efflux systems (2015)
  • Author(s): Mitsuko Hayashi-Nishino and Kunihiko Nishino
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2015
  • Place of Presentation: ハワイ（アメリカ合衆国）
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 多剤耐性病原細菌の新規治療戦略 (2015)
  • Author(s): 西野美都子
  • Organizer: 附置研究所間アライアンスによるナノとマクロをつなぐ 物質・デバイス・システム創製戦略プロジェクト 医療材料・デバイス・システムグループG3 分科会
  • Place of Presentation: 大阪府・大阪市
  • Year and Date: 2015-11-12
• [Presentation] サルモネラ薬剤自然抵抗性における薬剤排出ポンプとLPSの関係 (2015)
  • Author(s): 山崎聖司、西野美都子、西野邦彦
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] トランスポーター制御による細菌恒常性維持機構の解明と新規治療戦略の開発 (2014)
  • Author(s): 西野邦彦、Aixin Yan、林克彦、山崎聖司、福島愛子、松本佳巳、西野美都子、Danny KC Fung, Ma Yongzheng, Sun Jingjing, Gao Xiang, Kai Yong, Deng Ziqing
  • Organizer: 大阪大学国際共同研究促進プログラム報告会
  • Place of Presentation: 大阪
  • Year and Date: 2014-12-09