Functional analysis of B. bronchiseptica gene products specifically induced in rat trachea

• Project/Area Number: 26460525
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: Abe Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 病原生遺伝子 / 百日咳菌 / 気管支敗血症菌 / 病原性遺伝子

Outline of Final Research Achievements

Bordetella bronchiseptica, a Gram-negative pathogenic bacterium causing respiratory infections in a broad range of mammals, is considered to have an evolutionary progenitor-like relationship to Bordetella pertussis and Bordetella parapertussis, which are causative agents of pertussis in humans. Previous comparative genomic analysis revealed that genetic differences may be associated with adaptation to the different hosts and different manifestations of disease. To get a clue to understand the underlying genetic differences associated with broad host range of B. bronchiseptica, we constructed several deletion mutant of genes specifically induced in rat trachea or with long-range deletion of B. bronchiseptica specific gene loci and examined whether these loci are involved in bacterial colonization of rat trachea. However, no apparent difference was found between wild-type B. bronchiseptica and mutants in its ability to establish respiratory infection in rats.

Research Products

• [Journal Article] The bvg-repressed gene brtA, encoding biofilm-associated surface adhesin, is expressed during host infection by Bordetella bronchiseptica. (2016)
  • Author(s): Nishikawa S, Shinzawa N, Nakamura K, Ishigaki K, Abe H, Horiguchi Y.
  • Journal Title: Microbiol Immunol. Volume: 60 Issue: 2 Pages: 93-105
  • DOI: 10.1111/1348-0421.12356
  • NAID: 40020747667
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method. (2015)
  • Author(s): Abe H, Kamitani S, Fukui-Miyazaki A, Shinzawa N, Nakamura K, and Horiguchi Y.
  • Journal Title: Microbiology and Immunology Volume: 印刷中 Issue: 5 Pages: 249-261
  • DOI: 10.1111/1348-0421.12247
  • NAID: 40020479086
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Polymorphisms Influencing Expression of Dermonecrotic Toxin in Bordetella bronchiseptica. (2015)
  • Author(s): Okada K, Abe H, Ike F, Ogura Y, Hayashi T, Fukui-Miyazaki A, Nakamura K, Shinzawa N, Horiguchi Y.
  • Journal Title: PLoS One Volume: 10 Issue: 2 Pages: e0116604-e0116604
  • DOI: 10.1371/journal.pone.0116604
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of diphtheria toxin R domain mutants with enhanced inhibitory activity against HB-EGF (2015)
  • Author(s): Suzuki K, Mizushima H, Abe H, Iwamoto R, Nakamura H, Mekada E
  • Journal Title: J Biochem Volume: mvu079 Issue: 5 Pages: 331-343
  • DOI: 10.1093/jb/mvu079
  • NAID: 40020461422
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence of Bordetella bronchiseptica S798, an isolate from a pig with atrophic rhinitis (2014)
  • Author(s): Keisuke Okada, Yoshitoshi Ogura, Tetsuya Hayashi, Akio Abe, Asaomi Kuwae, Yasuhiko Horiguchi, Hiroyuki Abe
  • Journal Title: Genome Announcements Volume: 2 Issue: 3
  • DOI: 10.1128/genomea.00436-14
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] New insights from infection-specific gene expression network (2014)
  • Author(s): 安倍裕順、相川知宏、中鉢 淳、宮腰昌利、丸山史人
  • Journal Title: Nippon Saikingaku Zasshi Volume: 69 Issue: 3 Pages: 539-546
  • DOI: 10.3412/jsb.69.539
  • NAID: 130004688032
  • ISSN: 0021-4930, 1882-4110
  • Peer Reviewed / Open Access
• [Journal Article] A novel small regulatory RNA enhances cell motility in enterohemorrhagic <i>Escherichia coli</i> (2014)
  • Author(s): Sudo N, Soma A, Muto A, Iyoda S, Suh M, Kurihara N, Abe H, Tobe T, Ogura Y, Hayashi T, Kurokawa K,Ohinishi M, Sekine Y
  • Journal Title: The Journal of General and Applied Microbiology Volume: 60 Issue: 1 Pages: 44-50
  • DOI: 10.2323/jgam.60.44
  • NAID: 130004802264
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed
• [Presentation] Seven large loci specific to Bordetella bronchiseptica are not required for bacterial colonization in rat trachea. (2015)
  • Author(s): Hiroyuki Abe and Yasuhiko Horiguchi
  • Organizer: 第14回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 兵庫県淡路夢舞台
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] 気管支敗血症菌の皮膚壊死毒素DNTの産生量に関わる塩基多型の解析 (2014)
  • Author(s): 岡田圭祐, 安倍裕順, 小椋義俊, 林哲也, 堀口安彦
  • Organizer: 第67回日本細菌学会関西支部総会
  • Place of Presentation: 兵庫医科大学
  • Year and Date: 2014-11-22
• [Presentation] Complete genome sequence of Bordetella bronchiseptica S798, an isolate from a pig infected with atrophic rhinitis. (2014)
  • Author(s): Keisuke Okada, Hiroyuki Abe, Yoshitoshi Ogura, Tetsuya Hayashi, Akio Abe, Asaomi Kuwae, Yasuhiko Horiguchi
  • Organizer: あわじしま感染症・免疫フォーラム in 奈良
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2014-09-23 – 2014-09-26