| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Vibrio parahaemolyticus, a seafood-borne pathogen, elicits cytotoxicity in a manner dependent on its type III secretion system 1 (T3SS1). In this study, intracellular localization and toxicity of T3SS1-effectors of V. parahaemolyticus were analyzed. Expression of AMPlyation-inactive mutants of VopS in HeLa cells still induced cell morphological changes. Although an N-terminal plasma membrane-localizing domain of VopS alone did not induced cell morphological changes, the deletion of the N-terminal domain in VopS AMPlyation-inactive mutants impaired the toxicity. These results suggested that VopS exhibits AMPlyation-independent toxicity inside host cells, which is required for the localization to the plasma membrane.
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