Analysis of cytotoxic T3SS1 effectors of Vibrio parahaemolyticus

• Project/Area Number: 26460526
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Osaka University
• Principal Investigator: Matsuda Shigeaki 大阪大学, 微生物病研究所, 助教 (30506499)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 腸炎ビブリオ / ３型分泌装置 / エフェクター / 細胞死

Outline of Final Research Achievements

Vibrio parahaemolyticus, a seafood-borne pathogen, elicits cytotoxicity in a manner dependent on its type III secretion system 1 (T3SS1). In this study, intracellular localization and toxicity of T3SS1-effectors of V. parahaemolyticus were analyzed. Expression of AMPlyation-inactive mutants of VopS in HeLa cells still induced cell morphological changes. Although an N-terminal plasma membrane-localizing domain of VopS alone did not induced cell morphological changes, the deletion of the N-terminal domain in VopS AMPlyation-inactive mutants impaired the toxicity. These results suggested that VopS exhibits AMPlyation-independent toxicity inside host cells, which is required for the localization to the plasma membrane.

Research Products

• [Journal Article] Crystal structure of the ADP-ribosylating component of BEC, the binary enterotoxin of Clostridium perfringens. (2016)
  • Author(s): Kawahara K, Yonogi S, Munetomo R, Oki H, Yoshida T, Kumeda Y, Matsuda S, Kodama T, Ohkubo T, Iida T, Nakamura S
  • Journal Title: Biochem Biophys Res Commun Volume: 480 Issue: 2 Pages: 261-267
  • DOI: 10.1016/j.bbrc.2016.10.042
  • Peer Reviewed / Open Access
• [Journal Article] Genetic diversity of Vibrio parahaemolyticus strains isolated from farmed Pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in Thailand (2016)
  • Author(s): Chonsin K, Matsuda S, Theethakaew C, Kodama T, Junjhon J, Suzuki Y, Suthienkul O, Iida T
  • Journal Title: FEMS Microbiol Lett Volume: 363 Issue: 2 Pages: fnv222-fnv222
  • DOI: 10.1093/femsle/fnv222
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of Vibrio parahaemolyticus T3SS2 gene expression and function of T3SS2 effectors that modulate actin cytoskeleton. (2015)
  • Author(s): Kodama T, Hiyoshi H, Okada R, Matsuda S, Gotoh K, Iida T.
  • Journal Title: Cellular Microbiology Volume: 17(2) Issue: 2 Pages: 183-90
  • DOI: 10.1111/cmi.12408
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Interaction between the Type III Effector VopO and GEF-H1 Activates the RhoA-ROCK Pathway. (2015)
  • Author(s): Hiyoshi H, Okada R, Matsuda S, Gotoh K, Akeda Y, Iida T, Kodama T*
  • Journal Title: PLoS Pathogens Volume: 11(3) Issue: 3 Pages: e1004694-e1004694
  • DOI: 10.1371/journal.ppat.1004694
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] BEC, a novel enterotoxin of Clostridium perfringens found in human clinical isolates from acute gastroenteritis outbreaks (2014)
  • Author(s): Shinya Yonogi, Shigeaki Matsuda, Takao Kawai, Tomoko Yoda, Tetsuya Harad, Yuko Kumeda, Kazuyoshi Gotoh, Hirotaka Hiyoshi, Shota Nakamura, Toshio Kodama, Tetsuya Iida
  • Journal Title: Infection and Immunity Volume: 82 Issue: 6 Pages: 999-999
  • DOI: 10.1128/iai.01759-14
  • Peer Reviewed / Open Access