Structure-function analysis of bacterial toxins in tissue destruction to apply their functional domains to regenerative medicine
Project/Area Number |
26460527
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bacteriology (including mycology)
|
Research Institution | Okayama University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
内田 健太郎 北里大学, 医学部, 講師 (50547578)
美間 健彦 岡山大学, 医歯薬学総合研究科, 助教 (80596437)
|
Research Collaborator |
SAKON Joshua University of Arkansas・Fulbright college of Arts and Sciences, Chemistry and Biochemistry Department, Professor
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 細菌毒素 / コラーゲン結合ドメイン / 細胞外マトリックス / 膠原線維 / アンカリング / 再生医療 / 骨新生 / 技術移転 / アンカーリング |
Outline of Final Research Achievements |
Flesh-eating bacteria produce collagenases to degrade collagen fibrils and to destruct the host tissue. The enzymes consist of a catalytic domain, PKD domain(s) (PKD), and collagen-binding domain(s) (CBD). Since PKD enhanced the binding of CBD to insoluble collagen, PKD-CBD region derived from a collagenase was used to anchor growth factors to collagenous matrix, e.g. high-density collagen sheet or demineralized bone matrix. These composite materials were utilized to induce osteogenesis at bone defect sites. 3D-structure of PKD was determined by X-ray crystallography, which gave an insight into the molecular basis of the collagen anchoring. Furthermore, dimeric CBD was shown to be an appropriate anchor for a synthetic collagenous matrix.
|
Report
(4 results)
Research Products
(24 results)
-
-
-
[Journal Article] Oriented collagen tubes combined with basic fibroblast growth factor promote peripheral nerve regeneration in a 15 mm sciatic nerve defect rat model2017
Author(s)
Fujimaki H, Uchida K, Inoue G, Miyagi M, Nemoto N, Saku T, Isobe Y, Inage K, Matsushita O, Yagishita S, Sato J, Takano S, Sakuma Y, Ohtori S, Takahashi K, Takaso M.
-
Journal Title
J Biomed Mater Res A
Volume: 105
Issue: 1
Pages: 8-14
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Effect of Freeze-Dried Allograft Bone with Human Basic Fibroblast Growth Factor Containing a Collagen-Binding Domain From Clostridium Histolyticum Collagenase on Bone Formation After Lumbar Posterolateral Fusion Surgery in Rats2017
Author(s)
Inoue G, Uchida K, Matsushita O, Fujimaki H, Saito W, Miyagi M, Sekiguchi H, Nishi N, Ohtori S, Yogoro M, Takaso M.
-
Journal Title
Spine
Volume: 印刷中
Issue: 17
Pages: 995-1001
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
-
[Journal Article] Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects.2014
Author(s)
Ueno M, Uchida K, Saito W, Matsushita O, Yogoro M, Nishi N, Ogura T, Hattori S, Inoue G, Tanaka K, Takahira N, Takaso M.
-
Journal Title
Biomed Mater.
Volume: 9
Issue: 3
Pages: 035014-035014
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
-
-
[Journal Article] Acceleration of bone formation during fracture healing by injectable collagen powder and human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase2013
Author(s)
Saito W, Uchida K, Ueno M, MatsushitaO, Inoue G, Nishi N, Ogura T, Hattori S, Fujimaki H, Tanaka K, Takaso M
-
Journal Title
J Biomed Mater Res A
Volume: in press
Issue: 9
Pages: 3049-3055
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
[Patent(Industrial Property Rights)] 神経再生用移植材料、神経再生用移植材料の製造方法、及び神経再生用移植材料製造用キット2015
Inventor(s)
内田,井上,藤巻,高相,佐久,礒部,松下,美間,西,服部,田中他
Industrial Property Rights Holder
北里研究所、香川大学、株)アトリー、株)ニッピ
Industrial Property Rights Type
特許
Filing Date
2015-10-16
Related Report
Overseas
-