Role of the sugar metabolism of resident microbiota in the gut homeostasis

• Project/Area Number: 26460530
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Kagawa University
• Principal Investigator: KUWAHARA Tomomi 香川大学, 医学部, 教授 (60263810)
• Co-Investigator(Kenkyū-buntansha): 橋本 雅仁 鹿児島大学, 理工学域工学系, 教授 (30333537) ; 今大路 治之 (中山治之 / 中山 治之) 香川大学, 医学部, 助教 (80294669)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 腸管内常在菌 / Bacteroides / Clostridium difficile / 毒素 / 溶菌 / 糖代謝 / 腸内細菌 / 莢膜多糖 / Clostridium / 莢膜 / 構造解析 / 共生 / 感染防御 / 多糖 / 偽膜性大腸炎

Outline of Final Research Achievements

Inhibitory effect of Bacteroides thetaiotaomicron (BT) on the toxinogenicity of Clostridium difficile was evaluated in the co-infection model using BALB/c germ-free mice. The fecal Toxin A level in the mice infected with wild type BT and CD was lower than those in the mice colonized with CD and PS6- or polysaccharide transport-deficient BT. This result indicates that the BT inhibition on CD toxin production is mediated by BT polysaccharides. Cytotoxicity assay to HT-29 cells with the fecal supernatant showed the similar results as described above. In addition, the number of CD cell stained in Gram-positive was higher in the feces from the mice infected with wild BT and CD than the mice in other groups. These results indicate that the BT inhibition on CD toxin production is due to the suppression of CD autolysis by BT polysaccharides.

Research Products

• [Journal Article] Identification of a New Virulent Clade in Enterohemorrhagic Escherichia coli O26:H11/H- Sequence Type 29. (2017)
  • Author(s): Ishijima N, Lee KI, Kuwahara T, Nakayama-Imaohji H, Yoneda S, Iguchi A, Ogura Y, Hayashi T, Ohnishi M, Iyoda S.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 43136-43136
  • DOI: 10.1038/srep43136
  • Peer Reviewed / Open Access
• [Journal Article] Counterselection employing mutated pheS for markerless genetic deletion in Bacteroides species (2016)
  • Author(s): Kino Y, Nakayama-Imaohji H, Fujita M, Tada A, Yoneda S, Murakami K, Hashimoto M, Hayashi T, Okazaki K, Kuwahara T.
  • Journal Title: Anaerobe Volume: 42 Pages: 81-88
  • DOI: 10.1016/j.anaerobe.2016.09.004
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 腸内フローラに基づく腸内機能評価 (2016)
  • Author(s): 田中 彩, 下野隆一, 今大路治之, 鈴木基生, 桑原知巳
  • Journal Title: 日本静脈経腸栄養学会雑誌 Volume: 31 Pages: 1095-1098
  • Peer Reviewed
• [Journal Article] DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis. (2016)
  • Author(s): Nakayama-Imaohji H, Hirota K, Yamasaki H, Yoneda S, Nariya H, Suzuki M, Secher T, Miyake Y, Oswald E, Hayashi T, Kuwahara T
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0148887-e0148887
  • DOI: 10.1371/journal.pone.0148887
  • NAID: 120006534969
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] X-ray structure of a novel endolysin encoded by episomal phage phiSM101 of Clostridium perfringens (2014)
  • Author(s): Tamai, E., Yoshida, H., Sekiya, H., Nariya, H., Miyata, S., Okabe, A., Kuwahara, T., Maki, J. & Kamitori, S
  • Journal Title: Mol. Microbiol Volume: 92 Issue: 2 Pages: 326-337
  • DOI: 10.1111/mmi.12559
  • Peer Reviewed
• [Journal Article] Development of a novel plasmid vector pTIO-1 adapted for electrotransformation of Porphyromonas gingivalis. (2014)
  • Author(s): Tagawa J, Inoue T, Naito M, Sato K, Kuwahara T, Nakayama M, Nakayama K, Yamashiro T, Ohara N.
  • Journal Title: J Microbiol Methods Volume: 105 Pages: 174-179
  • DOI: 10.1016/j.mimet.2014.07.032
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 潰瘍性大腸炎患者の腸内細菌と自己血清IgGとの反応性 (2017)
  • Author(s): 桑原知巳、森枝千尋、今大路治之、石川秀樹、岡崎勝一郎
  • Organizer: 第90回日本細菌学会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-19
• [Presentation] Bacteroides fragilisにおけるシアリダーゼとエステラーゼは腹腔内膿瘍形成に関与する。2015年10月 (2015)
  • Author(s): 桑原知巳、今大路治之
  • Organizer: 第85回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 奈良、
  • Year and Date: 2015-10-15
• [Presentation] 腸内フローラと感染症 (2014)
  • Author(s): 桑原知巳
  • Organizer: 第84回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2014-10-23 – 2014-10-25
• [Presentation] 腸管内常在菌によるClostridium difficileの病原性抑制機構 (2014)
  • Author(s): 桑原知巳、今大路治之
  • Organizer: 日本細菌学会中国四国支部総会
  • Place of Presentation: 徳島
  • Year and Date: 2014-10-05 – 2014-10-06