Elucidation of caspase-1 activation inhivition mechanism by Vibrio parahaemolyticus effector VopQ

• Project/Area Number: 26460531
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: University of the Ryukyus
• Principal Investigator: HIGA Naomi 琉球大学, 医学(系)研究科(研究院), 技術専門職員 (70457688)
• Co-Investigator(Kenkyū-buntansha): 高江洲 義一 琉球大学, 熱帯生物圏研究センター, 准教授 (60403995)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Toshihiko 東京医科歯科大学, 医歯学総合研究科, 教授 (10292848)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マクロファージ / インフラマゾーム / 感染防御 / 感染防御・制御 / 炎症

Outline of Final Research Achievements

The effector protein VopQ of Vibrio parahaemolyticus is known to inhibit the activation of caspase-1 in macrophage inflammasome. I attempted to clarify, the relationship between autophagy induction and inflammasome activation inhibition, searching for target molecules for VopQ in host cells. As a result, the cell adhesion factor Afadin was a candidate the target molecule for VopQ, but interaction in macrophages was not observed. In addition, I could not identify such concerning the relation with autophagy, during this reserch period, but ATG5 conditional knockout mouse expressing specifically on phagocytic cells was made, so it can be expected to be utilized in future research.

Research Products

• [Remarks] 琉球大学医学研究科細菌学講座
  • URL: http://w3.u-ryukyu.ac.jp/bacteriology/